Myosin phosphorylation enhances rate of force development in fast-twitch skeletal muscle

Vandenboom, Rene; Grange, Robert W.; Houston, M. E.

doi:10.1152/ajpcell.1995.268.3.c596

Cited by 79 publications

(53 citation statements)

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“…The mechanism of PAP, myosin regulatory light chain phosphorylation, is most pronounced immediately after muscle activity, and then declines (Houston and Grange 1990;Houston et al 1985;Vandenboom et al 1995). In response to relatively brief muscle activity, such as a 10-s MVC of the knee extensor muscles, the manifestation of the PAP mechanism, twitch potentiation, is also greatest immediately after the MVC and then declines exponentially (Folland et al 2008;Hamada et al 2000;Houston and Grange 1990;Rassier and Herzog 2001).…”

Section: Discussionmentioning

confidence: 99%

“…The most common indicator of PAP is increased evoked isometric twitch force (twitch potentiation) observed following an evoked isometric tetanic contraction (O'Leary et al 1997) or a maximal voluntary isometric contraction (MVC) (Vandervoort et al 1983). More relevant to performance of fast movements is that PAP increases the rate of force development of evoked isometric tetanic contractions (Baudry and Duchateau 2007a;MacIntosh et al 2008;Vandenboom et al 1995), the force and power of evoked high velocity shortening (concentric) contractions (Abbate et al 2000), and the maximum velocity attained by evoked shortening contractions under load (Baudry and Duchateau 2007b;MacIntosh et al 2008). In addition to its effects on evoked (involuntary) contractions, PAP has been associated with enhanced voluntary contraction performance such as increased isometric rate of force development (Baudry and Duchateau 2007a), increased torque of moderately high velocity isokinetic concentric contractions (Miyamoto et al 2010), and increased shortening velocity attained with various loads (Baudry and Duchateau 2007b).…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of jump performance after a 5-RM squat is associated with postactivation potentiation

2011

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Weight lifting exercise may induce postactivation potentiation (PAP), thereby enhancing performance of a subsequent biomechanically similar "explosive" movement. However, it has not been shown that weight lifting induces PAP, indicated as potentiation of muscle twitch force. Therefore, the present study tested whether a five repetition maximum squat (5-RM squat) both induced PAP and increased the height of subsequently performed counter-movement jumps (CMJs). Eleven male athletes completed four laboratory sessions on separate days. Two sessions determined whether the 5-RM squat induced PAP: in one, a quadriceps maximal twitch was evoked immediately before and 8 min after a set of five CMJs (control); in the other, a twitch was evoked before a CMJ set, which was followed by a 4-min rest, a 5-RM squat, a 4-min rest, and a second twitch. Another two sessions tested the effect of the 5-RM squat on jump performance: in one session, two sets of five CMJs were performed with an 8-min rest between the sets (control); in the second, a 5-RM squat was performed 4 min after the first set of CMJs, and then after another 4 min the second set of CMJs was performed. Neither twitch torque nor CMJ height changed in the control sessions (P > 0.05). In contrast, interpolation of the 5-RM squat increased (P < 0.05) both twitch torque (49.5 ± 7.8 to 54.8 ± 11.9 N m; i.e., PAP = 10.7%) and CMJ height (48.1 ± 5.6 to 49.5 ± 5.9 cm; 2.9%). Since PAP was present at the time when CMJ height increased, it was concluded that PAP may have contributed to the increased CMJ height after a 5-RM squat.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of jump performance after a 5-RM squat is associated with postactivation potentiation

2011

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“…The graphs (Figs 4 and 7) displaying the change in DT and DT tc-' during 10 Hz stimulation illustrate the similar pattern of change in these parameters among the various groups, and the apparent restoration of a control-type response in atrophied muscle treated with DS. Comparison of these graphs illustrates the importance of the rate of force development in effecting the staircase response (MacIntosh & Gardiner, 1987;Vandenboom, Grange & Houston, 1995). It is interesting to note that repetitive stimulation of TTX-treated muscle resulted in prolongation of tc (from 5 to 10 s) without corresponding enhancement of DT.…”

Section: Half-relaxation Timementioning

confidence: 93%

Inhibition of Ca2+ release in rat atrophied gastrocnemius muscle

Rassier

Tubman²,

MacIntosh³

1997

Experimental Physiology

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SUMMARYContractile parameters of a twitch contraction and changes in these parameters during repetitive stimulation are modified by muscle atrophy induced by tetrodotoxin (TTX). These altered parameters include developed tension (DT), contraction time (ta), half-relaxation time (tR,J), average rate of force development (DT tC 1) and peak rate of relaxation (DTdtmin-'). These modifications may be related to different Ca concentration transients in the myoplasm during muscle stimulation.We have used dantrolene sodium (DS) in TTX-treated rat gastrocnemius muscle to test this hypothesis. In situ isometric contractile responses of rat gastrocnemius muscle during repetitive stimulation at 10 Hz were analysed before and after administration of DS. After DS administration, twitch amplitude, tC tR5 and DTtc-1 decreased and DTdtmin-I relative to DT increased in atrophied muscle. During repetitive stimulation, a progressive enhancement in developed tension (staircase) was absent in atrophied muscle, but DT increased to 171 + 4 %, presenting a staircase response after DS treatment. This potentiation was accompanied by an increase in DT tc1 to 175.6 + 7 %. Inhibition of Ca2+release in atrophied muscle resulted in twitch contractile parameters and contractile responses to 10 Hz stimulation that were similar, in many respects, to those responses in control (non-atrophied) muscles.

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“…For example, work by Stull and colleagues showed that stimulation-induced elevations in the myosin phosphate content of rat hindlimb muscles correlated with the magnitude of PTP [22,26,29]. Subsequent studies have confirmed these associations in mouse and rabbit hindlimb muscle [30][31][32][45][46][47]. Perhaps the most compelling evidence that RLC phosphorylation is responsible for PTP comes from studies performed on mouse extensor digitorum longus (EDL) muscles devoid of the skeletal isoform of myosin light-chain kinase (skMLCK), the enzyme responsible for phosphorylating the RLC in mammalian skeletal muscle [40].…”

Section: Introductionmentioning

confidence: 98%

Myosin light-chain phosphorylation and potentiation of dynamic function in mouse fast muscle

Xeni

Gittings

Caterini

et al. 2011

Pflugers Arch - Eur J Physiol

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The intent of this study was to determine if the stimulation-induced increase or "potentiation" of dynamic function of mouse extensor digitorum longus muscle (in vitro 25°C) during work cycles is graded to myosin regulatory light-chain (RLC) phosphorylation. To do this, concentric force and muscle work output during sinusoidal length changes were determined before (unpotentiated) and after (potentiated) the application of conditioning stimuli (CS) producing incremental elevations in RLC phosphorylation from rest. Sine wave excursion was from 1.09 to 0.91 of L (o) with a period of 142 ms; stimulating muscles to twitch and generate force during these cycles produced plots of force × displacement termed work loops. Stimulation at 2.5-, 5.0-, and 100-Hz elevated RLC phosphorylation from 0.16±0.02 (rest) to 0.29±0.03, 0.45±0.02 and 0.56±0.02 mol phos per mole RLC, respectively (n= 6-7, P<0.05). These CS potentiated mean concentric force (at all lengths) to 1.14±0.02, 1.26±0.04 and 1.41±0.06 of pre-stimulus, control levels (all n= 5-7, P<0.05) while work was increased to 1.07±0.02, 1.17±0.02 and 1.34±0.03 of controls, respectively. In a No CS condition that did not elevate RLC phosphorylation, neither mean concentric force nor work was altered. Thus, strong correlations between RLC phosphorylation and mean concentric force and work support the hypothesis that this molecular mechanism modulates muscle power output. No length-dependence for concentric force potentiation was observed in any condition, an outcome suggesting that interactions between instantaneous variations in muscle length and shortening velocity during work cycles modulates the potentiation response.

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Myosin phosphorylation enhances rate of force development in fast-twitch skeletal muscle

Cited by 79 publications

References 0 publications

Enhancement of jump performance after a 5-RM squat is associated with postactivation potentiation

Enhancement of jump performance after a 5-RM squat is associated with postactivation potentiation

Inhibition of Ca2+ release in rat atrophied gastrocnemius muscle

Myosin light-chain phosphorylation and potentiation of dynamic function in mouse fast muscle

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