Myosin isoform transitions and physiological properties of regenerated and re-innervated soleus muscles of the rat

Davis, Charles H.F.; Harris, John B.; Nicholson, L. V. B.

doi:10.1016/0960-8966(91)90004-c

Cited by 52 publications

(38 citation statements)

References 16 publications

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“…This finding indicated that transduced myofibers had been protected from the rounds of degenerationregeneration associated with the muscle pathology. To confirm this result, we investigated the expression of a myogenic marker, the developmental isoform of the myosin heavy chain 34 (MHCd), in injected muscles. Transduced fibers never expressed MHCd whereas groups of nontransduced fibers often displayed staining for MHCd (data not shown) again indicating that only this population of fibers was undergoing regeneration.…”

Section: Discussionmentioning

confidence: 93%

Early adenovirus-mediated gene transfer effectively prevents muscular dystrophy in alpha-sarcoglycan-deficient mice

et al. 2000

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Limb-girdle muscular dystrophy type 2D (LGMD 2D) is the most common cause of LGMD with a sarcoglycan defect. We recently engineered a murine model for this progressive disease and we investigated the possibility of preventing the development of muscular dystrophy in these animals by adenovirus-mediated gene transfer of human ␣-sarcoglycan.Here we report that a single intramuscular injection of a first generation adenovirus into the skeletal muscle of neonate mice led to sustained expression of ␣-sarcoglycan at the sarcolemma of transduced myofibers for at least 7 months. The morphology of transduced muscles was consequently pre-

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Section: Discussionmentioning

confidence: 93%

Early adenovirus-mediated gene transfer effectively prevents muscular dystrophy in alpha-sarcoglycan-deficient mice

et al. 2000

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“…They represented about 1% of the total myofiber population in these muscle biopsies. 11 Taken together, these examples provide evidence that small myofibers may occur as the result of chronic denervation.…”

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confidence: 87%

“…7 Muscle biopsies from patients with spinal cord injury lasting from 9 months to 19 years also contained immature fibers, which were interpreted as regenerative in nature. 8,11,14,15 These fibers had a central nucleus and a thin cytoplasmic rim containing a few myofilaments, and expressed the embryonic isoform of the myosin heavy chain molecule (MHCemb). They represented about 1% of the total myofiber population in these muscle biopsies.…”

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confidence: 99%

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Myogenesis in human denervated muscle biopsies

2007

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Electron microscopic studies of long-term denervated rat muscles have identified very small, immature myofibers that are believed to arise from detached satellite cells that have fused to form new fibers within the interstitial space. At present, it is unknown whether and to what extent equivalent fibers exist in denervated human muscle. Serial sections of muscle biopsies from 66 patients diagnosed with polyneuropathy or amyotrophic lateral sclerosis were immunolabeled with anti-NCAM and antineonatal myosin heavy chain monoclonal antibodies that are both neurally and developmentally regulated. We evaluated 200 myofibers in each section. Of the biopsy specimens, 75% contained small myofibers that showed a thin perinuclear cytoplasmic rim. Small fibers expressing neonatal myosin heavy chain (MHCnϩ) were found in all of these biopsies (100%) and NCAMϩ fibers in 98%. The percentage of MHCnϩ small fibers averaged 82% and NCAMϩ small myofibers averaged 40%. The percentage of NCAMϩ small fibers was significantly lower than that of MHCnϩ fibers. In contrast, the percentage of MHCnϩ vs. NCAMϩ angular atrophic fibers did not show a significant difference. A substantial subset of neurogenic biopsies showed small fibers that differ from angular atrophic fibers both in size and expression pattern of MHCn and NCAM. Myogenesis appears to be a frequent finding in neurogenic atrophy.

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“…Notexin destroys myofibers and motor endplate structures, leaving satellite cells and axons essentially intact (Harris et al 1975(Harris et al ,2000Davis et al 1991;Grubb et al 1991). Motor endplates are reestablished on the surface of the regenerating muscle fibers in a few days, again building contacts to nerve terminals in intact innervation (Harris et al 2000).…”

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confidence: 99%

Regeneration of Reinnervated Rat Soleus Muscle Is Accompanied by Fiber Transition Toward a Faster Phenotype

Mendler

Pintér

Kiricsi

et al. 2007

J Histochem Cytochem.

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S U M M A R Y The functional recovery of skeletal muscles after peripheral nerve transection and microsurgical repair is generally incomplete. Several reinnervation abnormalities have been described even after nerve reconstruction surgery. Less is known, however, about the regenerative capacity of reinnervated muscles. Previously, we detected remarkable morphological and motor endplate alterations after inducing muscle necrosis and subsequent regeneration in the reinnervated rat soleus muscle. In the present study, we comparatively analyzed the morphometric properties of different fiber populations, as well as the expression pattern of myosin heavy chain isoforms at both immunohistochemical and mRNA levels in reinnervated versus reinnervated-regenerated muscles. A dramatic slow-tofast fiber type transition was found in reinnervated soleus, and a further change toward the fast phenotype was observed in reinnervated-regenerated muscles. These findings suggest that the (fast) pattern of reinnervation plays a dominant role in the specification of fiber phenotype during regeneration, which can contribute to the long-lasting functional impairment of the reinnervated muscle. Moreover, because the fast II fibers (and selectively, a certain population of the fast IIB fibers) showed better recovery than did the slow type I fibers, the faster phenotype of the reinnervated-regenerated muscle seems to be actively maintained by selective yet undefined cues. (J Histochem Cytochem 56:111-123, 2008)

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Myosin isoform transitions and physiological properties of regenerated and re-innervated soleus muscles of the rat

Cited by 52 publications

References 16 publications

Early adenovirus-mediated gene transfer effectively prevents muscular dystrophy in alpha-sarcoglycan-deficient mice

Early adenovirus-mediated gene transfer effectively prevents muscular dystrophy in alpha-sarcoglycan-deficient mice

Myogenesis in human denervated muscle biopsies

Regeneration of Reinnervated Rat Soleus Muscle Is Accompanied by Fiber Transition Toward a Faster Phenotype

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