“…The mice were produced at the Centro de Investigación y de Estudios Avanzados (Mexico City, Mexico) animal facility, and the animal care and use committee of Centro de Investigación y de Estudios Avanzados approved all experiments. Abs and reagents: R2652 (rabbit polyclonal IgG anti-Myo1c) and mT2 (mouse monoclonal IgG1 anti-Myo1c) (19), biotinylated NIM-R4 (monoclonal rat anti-MHC-II), biotinylated mouse anti-H-2K d (BD Pharmingen), biotinylated rat IgG or mouse IgG (Jackson ImmunoResearch), goat anti-lamin B, goat antiactin (both from Santa Cruz), mouse anti-b tubulin, Alexa488-labeled goat anti-rabbit IgG, HRP-anti-mouse IgG1 and HRP-anti-goat IgG, FITCphalloidin, Pacific Blue anti-B220, PE anti-CD3 (BD Pharmingen), DAPI, phalloidin-rhodamine (Molecular Probes), streptavidin-Cy3, streptavidin-HRP, streptavidin-Sepharose beads (Zymed), protein G-Sepharose beads (Invitrogen), and biotinylated cholera toxin B subunit (Sigma). Mouse cell lines: Ag8, BCL-1, WEHI-231, 70Z/3, BaF/3, 3T3, BW5147 and RMA-S, IC21, and P815.…”