Myosin 1b promotes cell proliferation, migration, and invasion in cervical cancer

Zhang, Han-Rong; Lai, Shu-Yu; Huang, Lijun; Zhang, Zhenfei; Liu, Jie; Zheng, Sulin; Ding, Ke; Bai, Xin; Zhou, Jue-Yu

doi:10.1016/j.ygyno.2018.01.024

Cited by 33 publications

(33 citation statements)

References 20 publications

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“…Among the eleven candidate genes selected according to the clustering results, LASP1, RAB11B, RUVBL1 and MYO1B are upregulated in human malignant tumors and contribute to cancer progression [20,[44][45][46]. Based on the GENT database, we showed that both LASP1 and RUVBL1 gene expression were significantly upregulated in pancreatic cancer tissues compared with that in normal pancreatic tissues, while RAB11B was not changed obviously, and MYO1B was significantly downregulated (Additional file 5: Figure S1A).…”

Section: Upregulation Of Lasp1 Contributed To the Aggressive Propertimentioning

confidence: 98%

ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis

Wang

Dai

Gong

et al. 2019

J Exp Clin Cancer Res

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Background: Pancreatic cancer is a highly lethal malignancy with poor prognosis. Anillin (ANLN), an actin binding protein, is upregulated and plays an important role in many malignant tumors. However, the precise role of ANLN in pancreatic cancer remains unclear. Methods: The expression of ANLN and its association with pancreatic cancer patient survival were analyzed using an online database and confirmed by immunohistochemistry. The ANLN protein expression in pancreatic cancer cell lines was detected by Western blot. Cell proliferation, colony formation and transwell assays in vitro and in vivo tumor growth were used to determine the role of ANLN in pancreatic cancer. Gene expression microarray analysis and a series of in vitro assays were used to elucidate the mechanisms of ANLN regulating pancreatic cancer progression.Results: We found that the ANLN expression was significantly upregulated in pancreatic cancer tissues and cell lines. The high expression of ANLN was associated with tumor size, tumor differentiation, TNM stage, lymph node metastasis, distant metastasis and poor prognosis in pancreatic cancer. ANLN downregulation significantly inhibited cell proliferation, colony formation, migration, invasion and tumorigenicity in nude mice. Meanwhile, we found that ANLN knockdown inhibited several cell-cell adhesion related genes, including the gene encoding LIM and SH3 protein 1 (LASP1). LASP1 upregulation partially reversed the tumor-suppressive effect of ANLN downregulation on pancreatic cancer cell progression. Moreover, we found that ANLN downregulation induced the expression of miR-218-5p which inhibited LASP1 expression through binding to its 3'UTR. We also found that ANLN-induced enhancer of zeste homolog 2 (EZH2) upregulation was involved in regulating miR-218-5p/LASP1 signaling axis. EZH2 upregulation or miR-218-5p downregulation partially reversed the tumor-suppressive effect of ANLN downregulation on pancreatic cancer cell progression. Conclusion: ANLN contributed to pancreatic cancer progression by regulating EZH2/miR-218-5p/LASP1 signaling axis. These findings suggest that ANLN may be a candidate therapeutic target in pancreatic cancer.

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Section: Upregulation Of Lasp1 Contributed To the Aggressive Propertimentioning

confidence: 98%

ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis

Wang

Dai

Gong

et al. 2019

J Exp Clin Cancer Res

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“…In our study, it suggests this lncRNA is interrelated to myosins, which are a large and diverse family of molecular motors important for cell migration and motility (53). In PCa, Myo1b, Myo6, Myo9b, Myo10, and Myo18a were expressed at higher levels in high metastatic potential cells, and especially Myo1b and Myo10 were expressed at higher levels in metastatic tumors (54)(55)(56). Changes in expression of several myosin isoforms may contribute to metastasis in PCa (54).The GO analysis in our study showed the enrichment in the muscle system process, muscle hypertrophy and muscle development while KEGG pathway research also revealed vascular smooth muscle contraction got involved in PCa specimens (Figure 4).…”

Section: Discussionmentioning

confidence: 60%

Characterization and validation of long noncoding RNAs as new candidates in prostate cancer

Wang

et al. 2020

Preprint

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Background: Long noncoding RNAs (lncRNAs) have been proved to be an important regulator in gene expression. In almost all kinds of cancers, lncRNAs participated in the process of pathogenesis, invasion, and metastasis. Meanwhile, compared with the large amounts of patients, there is rare knowledge about the role of lncRNAs in prostate cancer (PCa). Material/Method: In this study, lncRNA expression profiles of prostate cancer were detected by Agilent microarray chip, 5 pairs of case and control specimens were involved in. Differentially expressed lncRNAs were screened out by volcano plot for constructing lncRNA-miRNA-mRNA central network. Then, the top ten up-regulated and down-regulated lncRNAs were validated by qRT-PCR in another 5 tumor specimens and 7 para-cancerous/benign contrasts. Furthermore, we searched for the survival curve of the top 10 upregulated and downregulated lncRNAs. Results: A total of 817 differentially expressed lncRNAs were filtered out by the criteria of fold change (FC) and t-test p < 0.05. Among them, 422 were upregulated, whereas 395 were downregulated in PCa tissues. Gene ontology and KEGG pathway analyses showed that many lncRNAs were implicated in carcinogenesis. lnc-MYL2-4:1 (FC = 0.00141, p = 0.01909) and NR_125857 (FC = 59.27658, p = 0.00128) had the highest magnitude of change. The subsequent qPCR confirmed the expression of NR_125857 was in accordance with the clinical samples. High expression of PCA3, PCAT14 and AP001610.9 led to high hazard ratio while low expression of RP11-279F6.2 led to high hazard ratio. Conclusions: Our study detected a relatively novel complicated map of lncRNAs in PCa, which may have the potential to investigate for diagnosis, treatment and follow-up in PCa. Our study revealed the expression of NR_125857 in human PCa tissues was most up-regulated. Further studies are needed to investigate to figure out the mechanisms in PCa.

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“…In our study, it suggests this lncRNA is interrelated to myosins, which are a large and diverse family of molecular motors important for cell migration and motility [61]. In PCa, Myo1b, Myo6, Myo9b, Myo10, and Myo18a were expressed at higher levels in high metastatic potential cells, and especially Myo1b and Myo10 were expressed at higher levels in metastatic tumors [62][63][64]. Changes in expression of several myosin isoforms may contribute to metastasis in PCa [62].…”

Section: Discussionmentioning

confidence: 84%

A Novel Analysis of the Differentiated Expression of Long Noncoding RNAs Profiles in Human Prostate Cancer

Sun

et al. 2020

Preprint

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Background: Long noncoding RNAs (lncRNAs) have crucial roles in cancer biology. Increasing numbers of evidences have indicated that lncRNAs play an important role in the pathogenesis, invasion, and metastasis in almost all kinds of cancers. But, compared with the large amounts of patients, there is rare reports that showed the differential expression of lncRNAs in prostate cancer. Methods: In this study, lncRNA expression profiles were screened in PCa, by using 5 pairs of clinical specimens in PCa and matched non-PCa tissues with lncRNA chip. To affirm further clinical value, we extended the samples consisting of another 5 tumor specimens and 7 para-cancerous/benign contrasts by qRT-PCR in top 10 up-regulated and down-regulated lncRNAs. Results: A total of 817 lncRNAs were differentially expressed between PCa tumor and para-cancerous tissues (Fold Change ≥2.0, p < 0.05): 422 were upregulated, whereas 395 were downregulated in PCa tissues. Gene ontology and KEGG pathway analyses showed that many lncRNAs were implicated in carcinogenesis. Among differentially expressed lncRNAs, lnc-MYL2-4:1 (FC = 0.00141, p = 0.01909) and NR_125857 (FC = 59.27658, p = 0.00128) had the highest magnitude of change. The subsequent qPCR confirmed the expression of NR_125857 accorded with the clinical samples.Conclusions: Our study detected a relatively novel complicated map of lncRNAs in PCa, which may have the potential to investigate for diagnosis, treatment and follow-up in PCa. Our study revealed the expression of NR_125857 in human PCa tissues was most up-regulated. Further study of these meaningful candidates are need to research deep mechanisms.

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Myosin 1b promotes cell proliferation, migration, and invasion in cervical cancer

Cited by 33 publications

References 20 publications

ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis

ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis

Characterization and validation of long noncoding RNAs as new candidates in prostate cancer

A Novel Analysis of the Differentiated Expression of Long Noncoding RNAs Profiles in Human Prostate Cancer

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