Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics

Iuliano, Olga; Yoshimura, Azumi; Prospéri, Marie-Thérèse; Martin, René; Knölker, Hans‐Joachim; Coudrier, Evelyne

doi:10.1083/jcb.201703205

Cited by 29 publications

(29 citation statements)

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“…Thus, it is very likely that Myo1b also debranches and reorganizes the architecture of branched actin networks at the plasma membrane. This is supported by our in vivo observations that Myo1b is required for the formation of filopodia induced by EphB2 receptor stimulation (Prospéri et al, 2015) and controls the extension of branched actin networks in growth cones (Iuliano et al, 2018). By debranching actin networks nearby the plasma membrane, Myo1b may favor the formation of linear and parallel filaments in growth cones and thus facilitate filopodia initiation upon EphB2 receptor stimulation.…”

Section: Sliding On Myo1b Reduces Branching Dynamics and Induces Debrsupporting

confidence: 66%

“…Myosin1c stabilizes actin around the Golgi complex (Capmany et al, 2019). Myosin1b (Myo1b) regulates neuronal polarization and axon elongation by limiting the extension of branched actin networks and favoring actin bundles in growth cones (Iuliano et al, 2018). However, the mammalian myosins1 lack a SH3 domain or an acidic motif that could interact with Arp2/3.…”

Section: Introductionmentioning

confidence: 99%

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Myosin 1b Flattens and Prunes Branched Actin Filaments

Pernier

Morchain

Caorsi³

et al. 2020

Preprint

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Motile and morphological cellular processes require a spatially and temporally coordinated branched actin network that is controlled by the activity of various regulatory proteins including the Arp2/3 complex, profilin, cofilin and tropomyosin. We have previously reported that myosin 1b regulates the density of the actin network in the growth cone. Using in vitro F-actin gliding assays and total internal reflection fluorescence (TIRF) microscopy we show in this report that this molecular motor flattens the Arp2/3-dependent actin branches up to breaking them and reduces the probability to form new branches. This experiment reveals that myosin 1b can produce force sufficient enough to break up the Arp2/3-mediated actin junction. Together with the former in vivo studies, this work emphasizes the essential role played by myosins in the architecture and in the dynamics of actin networks in different cellular regions. KeywordsMyosin 1b, Myosin 2, branched actin, Arp2/3, gliding assay, TIRF microscopy, actin architecture J.P., P.B and E.C. designed the study. J.P. and A.M. performed TIRF experiments and analyzed data; V.C. performed the STORM experiments and analyzed the data; A.B. performed the EM experiments; H.B. purified myosin 1b; J.P, P.B and E.C., wrote the paper.

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Section: Sliding On Myo1b Reduces Branching Dynamics and Induces Debrsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Myosin 1b Flattens and Prunes Branched Actin Filaments

Pernier

Morchain

Caorsi³

et al. 2020

Preprint

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“…Bioinformatics analysis and pathway analyses revealed that RAC1, STMN1, and CAPN2 are associated with the regulation of the actin cytoskeleton. The Rac/WAVE/Arp2/3 pathway is essential for generating and maintaining lamentous pseudopods [18][19][20][21]. The Arp2/3 complex-mediated network stabilizes the protrusions and makes them robust [22].…”

Section: Discussionmentioning

confidence: 99%

miRNA-101 Regulates Filamentogenesis During Migration and Invasion of Cervical Cancer Cells

Zhang

Wudu

Huang

et al. 2020

Preprint

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Background: miRNAs play critical roles in cervical cancer (CC) progression. miRNA-101 is a tumour suppressor in several cancers. The aim of this study was to investigate the in vitro and in vivo effect of miRNA-101 on the biological behaviour of CC cells by analysing the formation of filamentous pseudopodia.Methods: Quantitative reverse-transcription-polymerase chain reaction was performed to analyse miRNA-101 expression in CC cells before and after its transfection. Cell counting kit-8, transwell migration, and wound healing assays were performed to investigate the effect of miRNA-101 on the malignant behaviour of CC cells in vitro. The impact of miRNA-101 on filopodia initiation in CC cells was investigated via scanning electron microscopy and TRITC-labelled phalloidin staining evaluation. In vivo analysis was performed using a nude mouse model of CC, established via subcutaneous tumour transplantation, to validate the role of miRNA-101 on CC malignant behaviour. One-way ANOVA with Fisher’s least significant difference post hoc test and t-test were used to evaluate statistical significance of differences between groups.Results: miRNA-101 was significantly downregulated in CC cell lines. Overexpression of miRNA-101 inhibited the malignant behaviours of CC cells in vitro, while it inhibited the formation of filopodia in CC cells. Similar results were observed using immunofluorescence and confocal microscopy.Conclusions: These results indicate that miRNA-101 inhibits CC migration and invasion. The present findings, combined with future research, will help provide a theoretical basis and novel insight for the clinical management of CC.

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“…treadmilling and Shootin-1 mediated coupling to substrate-bound L1-CAM (Katsuno and Sakumura, 2015). Waves are driven by actin dynamics and contain myosin-II and myosin-Ib (Iuliano et al, 2018;Mortal et al, 2017), but also depend on microtubule via doublecortin coupling (Ruthel and Banker, 1998;Tint et al, 2009). Actin waves have been proposed to promote axonal growth and branching (Flynn et al, 2009;Ruthel and Banker, 1999; but see Mortal et al, 2017), by promoting neurite widening and microtubule-based transport (Winans et al, 2016).…”

Section: Actin Waves Along Developing Axonsmentioning

confidence: 99%

The functional architecture of axonal actin

Papandréou

Leterrier

2018

Molecular and Cellular Neuroscience

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The cytoskeleton builds and supports the complex architecture of neurons. It orchestrates the specification, growth, and compartmentation of the axon: axon initial segment, axonal shaft, presynapses. The cytoskeleton must then maintain this intricate architecture for the whole life of its host, but also drive its adaptation to new network demands and changing physiological conditions. Microtubules are readily visible inside axon shafts by electron microscopy, whereas axonal actin study has long been focused on dynamic structures of the axon such as growth cones. Super-resolution microscopy and live-cell imaging have recently revealed new actin-based structures in mature axons: rings, hotspots and trails. This has caused renewed interest for axonal actin, with efforts underway to understand the precise organization and cellular functions of these assemblies. Actin is also present in presynapses, where its arrangement is still poorly defined, and its functions vigorously debated. Here we review the organization of axonal actin, focusing on recent advances and current questions in this rejuvenated field.

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Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics

Abstract: Single-headed myosin 1 proteins are known to be expressed in neurons, but their function in these cells is still unclear. Iuliano et al. show that myosin 1b plays a major role in neuronal symmetry breaking via modulation of growth cone actin distribution and the propagation of actin waves.

Cited by 29 publications

References 50 publications

Myosin 1b Flattens and Prunes Branched Actin Filaments

Myosin 1b Flattens and Prunes Branched Actin Filaments

miRNA-101 Regulates Filamentogenesis During Migration and Invasion of Cervical Cancer Cells

The functional architecture of axonal actin

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