Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression

Yang, Guokun; Liang, Xiaomin; Jiang, Yanle; Li, Chengquan; Hu, Jihong; Zhang, Yanmin; Zhang, Xindang; Chang, Xulu; Hu, Wenpan; Li, Keke; Meng, Xiaolin

doi:10.1016/j.aqrep.2022.101306

Cited by 2 publications

(4 citation statements)

References 26 publications

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“…In the refeeding group, the ccdc3 expression levels were considerably promoted compared with those in the fasting group. In our previous study, the serum glucose level decreased remarkably in the fasting group and increased in the refeeding group (Yang, Liang, Jiang, Li, Hu, et al, 2022). The ccdc3 mRNA level may be related to the serum glucose level in fasting and refeeding groups.…”

Section: Discussionmentioning

confidence: 81%

Cloning grass carp (Ctenopharyngodon idella) ccdc3 and its expression affected by nutrition state, insulin, and glucagon

Yang,

Wang

et al. 2023

Journal of Fish Biology

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As an adipokine, coiled‐coil domain‐containing 3 (CCDC3) plays multiple physiological functions in fatty liver, lipid metabolism, and abdominal obesity. Grass carp was selected as the experimental animal in this study to investigate the roles of Ccdc3 in teleost. Results showed that the open reading frame (ORF) of cloned ccdc3 was 831 bp and encoded 276 amino acids. Three N‐glycosylation sites and a predicted coiled‐coil domain motif were located in the identified Ccdc3. Moreover, a nuclear localization signal (NLS) was contained in the coiled‐coil domain motif of the identified Ccdc3. The results on tissue distribution revealed that ccdc3 was highly detected in grass carp fat and brain tissue. In the oral glucose tolerance test (OGTT), the expression of ccdc3 increased dramatically in the brain, hypothalamus, and visceral fat in the glucose treatment group. In the fasting and refeeding experiment, the ccdc3 expression levels were dramatically reduced in the brain, hypothalamus, and visceral fat after 14 days of fasting. In the refeeding group, the ccdc3 expression levels were considerably promoted compared with those in the fasting group. In the induced overfeeding experiment, the ccdc3 expression increased remarkably in the hepatopancreas, brain and visceral fat tissues. The ccdc3 expression in the primary hepatocytes were dramatically increased with glucose, oleic acid, and insulin treatment. However, ccdc3 expression were markedly decreased with glucagon treatment. In conclusion, these results indicates that Ccdc3 takes part in regulating glucose and lipid metabolism of teleost.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 81%

Cloning grass carp (Ctenopharyngodon idella) ccdc3 and its expression affected by nutrition state, insulin, and glucagon

Yang,

Wang

et al. 2023

Journal of Fish Biology

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“…In the OGTT, the experimental process was referred to the previous study [ 26 , 27 ]. The grass carp were domesticated for two weeks.…”

Section: Methodsmentioning

confidence: 99%

“…In the fast and refeeding experiment, the experimental procedure was referred to the previous study [ 27 , 28 ]. After acclimating for two weeks, the experiment was implemented.…”

Section: Methodsmentioning

confidence: 99%

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Molecular Characterization of Grass Carp GIPR and Effect of Nutrition States, Insulin, and Glucagon on Its Expression

Yang

Liang

Jiang

et al. 2022

Aquaculture Nutrition

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GIP plays an important regulatory role in glucose and lipid metabolism. As the specific receptor, GIPR is involved in this physiological process. To assess the roles of GIPR in teleost, the GIPR gene was cloned from grass carp. The ORF of cloned GIPR gene was 1560 bp, encoding 519 amino acids. The grass carp GIPR was the G-protein-coupled receptor which contains seven predicted transmembrane domains. In addition, two predicted glycosylation sites were contained in the grass carp GIPR. The grass carp GIPR expression is in multiple tissues and is highly expressed in the kidney, brain regions, and visceral fat tissue. In the OGTT experiment, the GIPR expression is markedly decreased in the kidney, visceral fat, and brain by treatment with glucose for 1 and 3 h. In the fast and refeeding experiment, the GIPR expression in the kidney and visceral fat tissue was significantly induced in the fast groups. In addition, the GIPR expression levels were markedly decreased in the refeeding groups. In the present study, the visceral fat accumulation of grass carp was induced by overfed. The GIPR expression was significantly decreased in the brain, kidney, and visceral fat tissue of overfed grass carp. In primary hepatocytes, the GIPR expression was promoted by treatment with oleic acid and insulin. The GIPR mRNA levels were significantly reduced by treatment with glucose and glucagon in the grass carp primary hepatocytes. To our knowledge, this is the first time the biological role of GIPR is unveiled in teleost.

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Myonectin in grass carp (Ctenopharyngodon idella): Molecular cloning and effect of nutrition state, insulin and glucagon on myonectin expression

Cited by 2 publications

References 26 publications

Cloning grass carp (Ctenopharyngodon idella) ccdc3 and its expression affected by nutrition state, insulin, and glucagon

Cloning grass carp (Ctenopharyngodon idella) ccdc3 and its expression affected by nutrition state, insulin, and glucagon

Molecular Characterization of Grass Carp GIPR and Effect of Nutrition States, Insulin, and Glucagon on Its Expression

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