Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR

Ribeiro, Márcio Garcia; Silva, Rodrigo; Pires, Prhiscylla Sadanã; Martinho, Anna Paula Vitirito; Lucas, Thays Mizuki; Teixeira, Ana Izabel Passarela; Paes, Antônio Carlos; Barros, Claudenice Batista; Lobato, Francisco Carlos Faria

doi:10.1016/j.anaerobe.2012.09.003

Cited by 14 publications

(19 citation statements)

References 17 publications

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“…In five cases (Bovines 1, 2, 11, 25, and 29) muscle samples were wrapped in hot paraffin to maintain anaerobiosis during transportation to the laboratory for isolation of pathogenic clostridia. Another three samples were refrigerated at 5°C for 12 (case 59, Table 1) to 36 (cases 52 and 54, Table1) months and then submitted to identification of the agent by bacterial culture followed by PCR according to previously described technique (Ribeiro et al 2012). PCR was also employed for identification of Clostridium chauvoei in FFPE samples from 12 cases (48-59, Table 1) using techniques previously described (Kojima et al 2001).…”

Section: Table1 Outbreaks Of Blackleg In Cattle From the State Of Matomentioning

confidence: 99%

Blackleg in cattle in the state Mato Grosso do Sul, Brazil: 59 cases

Heckler

Lemos²,

Gomes³

et al. 2018

Pesq. Vet. Bras.

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This study aimed to review cases of blackleg (Clostridium chauvoei infection) diagnosed in cattle from Midwestern Brazil from 1994 to 2014 considering epidemiological, clinical, necropsy and histopathological findings. Also the following laboratory tests were used for the diagnosis of some cases of blackleg: microbiological culture and identification of the agent, microbiological culture and identification of the agent by the polymerase chain reaction (PCR), and identification of the agent in formalin fixed paraffin embedded tissues (FFPE). Criteria for presumptive diagnosis of blackleg included necrohemorrhagic emphysematous myositis consisting of inflammatory infiltrate, coagulative necrosis of myofiber, interstitial edema, hemorrhage, and gas bubbles between myofibers. Fifty nine cases from 51 outbreaks of blackleg were found, which corresponded to 1.1% of 5,375 cattle deaths investigated. In five of those outbreaks, samples of affected muscles cultures for the identification of pathogenic clostridia were made. Another three samples of similar material were cultured for clostridia with subsequent identification of the isolate by PCR. Twelve samples of FFPE affected muscle fragments were submitted to PCR for identification of the etiological agent. Except for January, cases were observed in each month of the year, with higher numbers in July-October. Most affected cattle were in the age of 7-12 years, but calves younger than 6 month-old and older than 24 months were also observed. Vaccination histories were scarce. In 32 outbreaks some vaccination history was available, but only in two of those vaccination has been carried out properly. In 56 six cases the skeletal muscles were involved. Muscles of the hind limbs were the most affected. In ten cases muscles of the tongue, myocardium and diaphragm were also affected. In three of the cases the visceral form was observed. Deaths occurred after a clinical course of 6-24 hours, but in most cases cattle were found death. Sudden death was the outcome in visceral cases (cardiac) blackleg. Clostridium chauvoei was confirmed to be the cause by culturing in 5 cases, and by PCR and histopatology in 8 cases. Bacterial culture followed by PCR did not demonstrate C. chauvoei. Calculation of the economic impact indicates that blackleg is a frequent disease in the state of Mato Grosso do Sul (MS) that inflicts significant economic loss. The amount of these losses would be reduced through proper vaccination programs against the prevalent strains of C. chauvoei in the region.

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Section: Table1 Outbreaks Of Blackleg In Cattle From the State Of Matomentioning

confidence: 99%

Blackleg in cattle in the state Mato Grosso do Sul, Brazil: 59 cases

Heckler

Lemos²,

Gomes³

et al. 2018

Pesq. Vet. Bras.

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“…Then, the samples were incubated in an aerobic atmosphere (80% N 2 ; 10% CO 2 and 10% H 2 ) at 37°C for 48 hours. The colonies were subjected to morphological and colorimetric evaluation by Gram staining and evaluated by multiplex polymerase chain reaction (PCR) to detect C. chauvoei, C. perfringens type A, C. sordellii, C. novyi and C. septicum as described by RIBEIRO et al (2012).…”

Section: O Objetivo Deste Estudo Foi Relatar Um Caso De Miocardite CLmentioning

confidence: 99%

“…Unlike IHC, which is sufficient for diagnosis based on the in situ localization of the agent, isolation is not considered an essential step (UZAL et al, 2003). However, isolation followed by PCR or even direct PCR from the tissue could be a good option when sample was not well conserved with formalin (RIBEIRO et al, 2012). In addition, molecular identification by PCR will be critical in future epidemiological studies of the strains involved in this disease (HALM et al, 2010;RIBEIRO et al, 2012).…”

Section: O Objetivo Deste Estudo Foi Relatar Um Caso De Miocardite CLmentioning

confidence: 99%

“…However, isolation followed by PCR or even direct PCR from the tissue could be a good option when sample was not well conserved with formalin (RIBEIRO et al, 2012). In addition, molecular identification by PCR will be critical in future epidemiological studies of the strains involved in this disease (HALM et al, 2010;RIBEIRO et al, 2012). This is the first reported diagnosis of visceral blackleg in Brazil with primary myocarditis caused by C. chauvoei.…”

Section: O Objetivo Deste Estudo Foi Relatar Um Caso De Miocardite CLmentioning

confidence: 99%

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Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

et al. 2015

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The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR).

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“…30 There is also a case report about myonecrosis due to C septicum infection in a dog with myiasis. 31 In addition, gas gangrene caused by C septicum is reported in goats, considering endogenous infection due to pregnancy-induced immunosuppression due to the absence of skin lesions. 32 Similar lesions occur in blackleg disease of ruminants after oral infection with C chauvoei.…”

Section: Discussionmentioning

confidence: 99%

Fatal bacterial septicaemia after immobilisation of a captive brown bear (Ursus arctos)

Schlohsarczyk

Schmidt

Prenger‐Berninghoff

et al. 2020

Vet Record Case Reports

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A four‐year‐old female brown bear (Ursus arctos) was immobilised via blowpipe targeting the right hind limb to perform a chemical contraception. Few days after anaesthesia, the animal developed lameness and a reduced general condition, and died 1 week after immobilisation when alopecia and swelling of the injection area were observed for the first time. Besides a severe azotaemia, alopecia and reddening of the craniomedial skin, subcutis and skeletal muscles of the entire right hind limb showed a phlegmon, oedema and haemorrhage. Additional widespread haemorrhages were found. Histology revealed a suppurative and necrohaemorrhagic myositis of the affected limb, a suppurative iliofemoral lymphadenitis, blood resorption in the mesenteric lymph nodes and haemorrhage in the adrenal gland cortex. Microbiological investigation revealed high amounts of Escherichia coli, Streptococcus dysgalactiae and Clostridium septicum in skin, musculature, heart, lung, liver and spleen, indicating septicaemia, which led to death due to toxaemic shock.

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Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR

Cited by 14 publications

References 17 publications

Blackleg in cattle in the state Mato Grosso do Sul, Brazil: 59 cases

Blackleg in cattle in the state Mato Grosso do Sul, Brazil: 59 cases

Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

Fatal bacterial septicaemia after immobilisation of a captive brown bear (Ursus arctos)

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