Myometrial Responses to the Menstrual Plain-Muscle Stimulant

Pickles, V. R.

doi:10.1677/joe.0.0190150

Cited by 24 publications

(11 citation statements)

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“…2. This experiment confirms earlier observations (Pickles, 1959) in that a single addition of menstrual stimulant caused the rhythmical contractions of the preparation to become better co-ordinated and more powerful. Such a response might continue almost indefinitely, but in this instance it was inhibited by the addition of ambucetamide.…”

Section: Resultssupporting

confidence: 91%

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The Effects of Ambucetamide on Human Myometrial and Other Preparations, and Its Antagonism to the Menstrual Stimulant

Pickles

Clitheroe

1960

British Journal of Pharmacology and Chemotherapy

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A plain‐muscle stimulant, provisionally named the “menstrual stimulant,” may be involved in the causation of dysmenorrhoea. Since ambucetamide [α‐dibutylamino‐α‐(p‐methoxyphenyl)‐acetamide] has been claimed to alleviate this condition, its effects on the responses of human myometrial preparations to the menstrual stimulant have been studied. Ambucetamide regularly inhibited these responses, as well as those to vasopressin, and sometimes diminished the spontaneous rhythmical activity of the human myometrium. The rat and guinea‐pig uterus, the guinea‐pig and cat intestine preparations, showed more complex responses and may therefore be less suitable for testing antispasmodic drugs intended for treatment of dysmenorrhoea.

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Section: Resultssupporting

confidence: 91%

“…The " menstrual stimulant" is a plain-muscle stimulant of endometrial origin, overactivity of which is considered to be a possible cause of dysmenorrhoea (Chambers and Pickles, 1958;Pickles, 1959). It was therefore of interest to see whether ambucetamide antagonized the effect of menstrual stimulant on the human myometrium.…”

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confidence: 99%

The Effects of Ambucetamide on Human Myometrial and Other Preparations, and Its Antagonism to the Menstrual Stimulant

Pickles

Clitheroe

1960

British Journal of Pharmacology and Chemotherapy

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“…The general types of effect on smooth-muscle preparations have been described before (Chambers & Pickles, 1958;Pickles, 1959b) and only the following additional facts need be noted here. Although all these stimulants caused contractions that were slow and persistent by comparison with those induced by histamine, the responses to component C were often delayed in onset by j-2 min and then 234 lllsom 0 SEPARATION OF MENSTRUAL MUSCLE STIMULANTS 235 persisted for 5 min or more, even after the stimulant had been washed out (Fig.…”

Section: Resultsmentioning

confidence: 89%

“…It had previously been found (Pickles, 1959b) that no appreciable quantities of histamine, acetylcholine or 5-hydroxytryptamine, added to the initial acetone extract, passed into the crude lipid extract (then made with ether). A similar experiment showed that this was still true for histamine and 5-hydroxytryptamine when dichloromethane was used instead of ether, but that a small amount of acetylcholine could pass into the crude extract.…”

Section: H J Clitheroe and V R Picklesmentioning

confidence: 99%

The separation of the smooth‐muscle stimulants in menstrual fluid

Clitheroe¹,

Pickles²

1961

The Journal of Physiology

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“…Department of Physiology, University of Sheffield Human endometrial curettings and menstrual fluid contain lipoid substances that stimulate the myometrium in vitro. It is thought that these substances released in vivo would stimulate the myometrium and so facilitate the expulsion of the decidua menstrualis (Pickles, 1959).…”

Section: Oxfordmentioning

confidence: 99%

Proceedings of the Physiological Society

1961

The Journal of Physiology

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Standard techniques, such as that of Bradley & Elkes (1953) used for recording cortical electrical activity of adult animals are unsuitable in the young animal because the skull is too thin to support screw electrodes. Wire electrodes have been employed with young animals but have been restricted so far to acute experiments in which either the cerebral hemispheres have been exposed, the electrodes placed extradurally, or the animal restrained.The technique described here enables behaviour and electrocortical activity to be observed simultaneously, and is particularly suited for chronic studies in young or small adult animals. The electrodes used are light and flexible, and are of a simple type which can be made rapidly. One disadvantage of most electrodes used for recording the electrocorticogram is that cortical damage may occur at the point of electrode insertion, which is also the recording site. In this method recording is remote from the point of insertion and damage. The operative procedure is straightforward and the preparation can be completed in approximately 30 min. The technique has been used to record the electrocorticogram before and after the administration of drugs, of conscious, unrestrained guinea-pigs, chicks and kittens of 1 day or older.The guinea-pigs were anaesthetized with tribromoethanol 150 mg/kg, and the kittens by an intraperitoneal injection of pentobarbitone sodium 20 mg/kg. The guinea-pigs and kittens also received a dose of 0-25 or 0 5 mg of promazine by the same route. The chicks were anaesthetized with ether by means of a small face mask and the scalp was infiltrated with local anaesthetic (Xylotox, Pharmaceutical Manufacturing Co.). In all animals a mid-line incision was made with aseptic precautions and the underlying aponeurosis was divided and reflected to the sides. The periosteum was * Nuffield Foundation Medical Fellow, and B.M.A. Walter Dixon Memorial Scholar. c 30PPROCEEDINGS OF THE PHYSIOLOGICAL carefully scraped off the parietal bone except in the mid-line region because of the risk of opening the sagittal sinus. Two or three holes were then drilled through the parietal bone with a No. 1 round dental burr, 1-2 mm on each side of, and parallel to the mid line. A hole was also drilled through the frontal bone to take an earth electrode. The dura was pierced with a needle and the recording electrodes were inserted through the drill holes in the skull and directed laterally for 2-15 mm under the dura.The recording and earthing electrodes for the chick and guinea-pig were made from 20 in. (50 cm) lengths of 'Diamel '-coated 0 004 in. (0.1 mm) diameter nickel-chromium wire (Johnson, Matthey & Co. Ltd.). The tips were bared for 1 mm and bent upon themselves. The electrodes for the kittens were essentially of the same design, but only 3 in. (7.5 cm) long and made from 0*012 in. (0.3 mm) diameter 'Diamel'-coated silver wire (Johnson, Matthey and Co. Ltd.). The electrodes were sealed into place with 'Simplex' auto-polymerizing acrylic resin (Dental Fillings, Ltd) and led ...

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Myometrial Responses to the Menstrual Plain-Muscle Stimulant

Cited by 24 publications

References 0 publications

The Effects of Ambucetamide on Human Myometrial and Other Preparations, and Its Antagonism to the Menstrual Stimulant

The Effects of Ambucetamide on Human Myometrial and Other Preparations, and Its Antagonism to the Menstrual Stimulant

The separation of the smooth‐muscle stimulants in menstrual fluid

Proceedings of the Physiological Society

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