Myoinositol ameliorates high-fat diet and streptozotocin-induced diabetes in rats through promoting insulin receptor signaling

Antony, Poovathumkal James; Gandhi, Gopalsamy Rajiv; Stalin, Antony; Balakrishna, K.; Toppo, Erenius; Sivasankaran, Kuppusamy; Ignacimuthu, S.; Al-Dhabi, Naïf Abdullah

doi:10.1016/j.biopha.2017.01.170

Cited by 46 publications

(38 citation statements)

References 33 publications

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“…In regards to the insulin resistance index (HOMA-IR), the untreated diabetic group showed a significant increase in the IR when compared to the control animals. Similarly, previous studies [17,42] reported significantly higher HOMA-IR values in untreated diabetic rats compared to the control animals.…”

Section: Discussionsupporting

confidence: 79%

The Biological Impacts of Sitagliptin on the Pancreas of a Rat Model of Type 2 Diabetes Mellitus: Drug Interactions with Metformin

Shawky

Morsi

Bana

et al. 2019

Biology

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Sitagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, is a beneficial class of antidiabetic drugs. However, a major debate about the risk of developing pancreatitis is still existing. The aim of the work was to study the histological and immunohistochemical effects of sitagliptin on both endocrine and exocrine pancreases in a rat model of type 2 diabetes mellitus and to correlate these effects with the biochemical findings. Moreover, a possible synergistic effect of sitagliptin, in combination with metformin, was also evaluated. Fifty adult male rats were used and assigned into five equal groups. Group 1 served as control. Group 2 comprised of untreated diabetic rats. Group 3 diabetic rats received sitagliptin. Group 4 diabetic rats received metformin. Group 5 diabetic rats received both combined. Treatments were given for 4 weeks after the induction of diabetes. Blood samples were collected for biochemical assay before the sacrification of rats. Pancreases were removed, weighed, and were processed for histological and immunohistochemical examination. In the untreated diabetic group, the islets appeared shrunken with disturbed architecture and abnormal immunohistochemical reactions for insulin, caspase-3, and inducible nitric oxide synthase (iNOS). The biochemical findings were also disturbed. Morphometrically, there was a significant decrease in the islet size and islet number. Treatment with sitagliptin, metformin, and their combination showed an improvement, with the best response in the combined approach. No evidence of pancreatic injury was identified in the sitagliptin-treated groups. In conclusion, sitagliptin had a cytoprotective effect on beta-cell damage. Furthermore, the data didn’t indicate any detrimental effects of sitagliptin on the exocrine pancreas.

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Section: Discussionsupporting

confidence: 79%

The Biological Impacts of Sitagliptin on the Pancreas of a Rat Model of Type 2 Diabetes Mellitus: Drug Interactions with Metformin

Shawky

Morsi

Bana

et al. 2019

Biology

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“…Results illustrated in table 5 showed that regarding to diabetic group, s ignificant increases were obtained in liver function markers when compared to normal control one. In contrary, both diabetic treated groups showed significant decline in all tested parameters when compared to the diabetic group.…”

Section: Bm-mscs Flow Cytometric Characterizationmentioning

confidence: 88%

ASSESSMENT OF THE POTENTIAL AMELIORATING EFFECTS OF BM-MSCsOR INSULIN ON THE ALTERED METABOLIC STATUS OF PANCREAS, LIVER AND KIDNEYIN STZ-DIABETIC RATS.

ElKholy¹

2018

IJAR

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The current study was designed to investigate the probable hypoglycemic, hypolipidemic and hepato-renal protective effects of bone marrow derived mesenchymal stem cells (BM-MSCs) in comparison with insulin treat ment in diabetic rats. Animals were classified into 4 groups; control group, diabetic group (D) received a single IP STZ dose (45 mg/kg b.w), D + insulin (0.75 IU/100 g m bw,SC daily for 4 weeks) group and D + BM-MSCs (single IV dose of 10 6 cell/rat). Herein, both insulin and BM-MSCs administration significantly improved the hyperglycemic status resulting fro m d iabetes induction, as evidenced by lowered blood glucose, HbA1c and AGEs levels, while enhanced serum insulin, C-peptide and HO-1 levels, compared to the diabetic group. Regard ing lipid metabolism, the increased levels of lip id fractions (TL, TG, TC and LDL-C) and the reduced HDL-C level in diabetic rats were reverted back to near normal values as a consequence to BM-MSCs treatment; indicat ing further, its hypolipidemic effect; in addition to enhancing the protein metabolism in diabetic rats. Furthermore, BM-M SCs was found to has hepato-renal protective effects via improving liver functions in diabetic rats; confirmed by decreased serum AST, A LT, A LP and γ-GT activ ities associated with decreased total bilirubin but increased total protein and albumin levels; and improving kidney status; indicated by the decreased serum levels of creatinine, uric acid and urea; compared to the diabetic group. Current findings clearly point out the health benefits of BM -MSCs; mo re than insulin; in ameliorating various metabo lic disorders and hepato-renal diabetic co mp licat ions.

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“…According to all these algorithms, our results show that the three most stable genes were Hprt1, Tbp, and Rpl32 for IBAT and Tbp, B2m, and Hprt1 for RWAT, while the least stable genes were 18S, Actb, and Gapdh for both tissues. The Actb and Gapdh genes are widely used in the literature as normalizers for the evaluation of target gene expression in WAT and BAT (Aguirre et al, 2016;Antony et al, 2017;Calderon-Dominguez et al, 2016;Chang and Kim, 2017;Li et al, 2015;Rodrigues et al, 2017;Tsubai et al, 2016;Yan et al, 2017), however, a few authors argue about the stability of the reference genes. A systematic review showed that the vast majority of the studies use a single gene as a normalizer, with Actb and/or Gapdh being commonly selected to evaluate the gene expression in vertebrates (Chapman and Waldenstrom, 2015).…”

Section: Discussionmentioning

confidence: 99%

Identification of Suitable Reference Genes for Quantitative Gene Expression Analysis in Innervated and Denervated Adipose Tissue from Cafeteria Diet‐Fed Rats

Marques-Oliveira

Silva

Valadares

et al. 2019

Lipids

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Our previous studies show that cafeteria diet increases body adiposity, plasma insulin levels, and sympathetic activity to brown adipose tissue (BAT) and white adipose tissue (WAT) of Wistar rats, leading to rapid and progressive changes in the metabolic profile. The identification of suitable reference genes that are not affected by the experimental conditions is a critical step in accurate normalization of the reverse transcription quantitative real‐time PCR (qRT‐PCR), a commonly used assay to elucidate changes in the gene expression profile. In the present study, the effects of the cafeteria diet and sympathetic innervation on the gene expression of adrenoceptor beta 3 (Adrb3) from BAT and WAT were assessed using one of the most stable and one of the least stable genes as normalizers. Rats were fed the cafeteria diet and on the 17th day, interscapular BAT or retroperitoneal WAT was denervated and, 7 days after surgery, the contralateral innervated tissue was used as control. Ten reference genes were evaluated (18S, B2m, Actb, CypA, Gapdh, Hprt1, Rpl32, Tbp, Ubc, and Ywhaz) and ranked according to their stability using the following algorithms: geNorm, NormFinder, BestKeeper, and comparative delta threshold cycle (ΔC t) method. According to the algorithms employed, the normalization of Adrb3 expression by the least stable genes produced opposite results compared with the most stable genes and literature data. In cafeteria and control diet‐fed rats, the three most stable genes were Hprt1, Tbp, and Rpl32 for interscapular BAT and Tbp, B2m, and Hprt1 for retroperitoneal WAT, while the least stable genes were 18S, Actb, and Gapdh for both tissues.

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Myoinositol ameliorates high-fat diet and streptozotocin-induced diabetes in rats through promoting insulin receptor signaling

Cited by 46 publications

References 33 publications

The Biological Impacts of Sitagliptin on the Pancreas of a Rat Model of Type 2 Diabetes Mellitus: Drug Interactions with Metformin

The Biological Impacts of Sitagliptin on the Pancreas of a Rat Model of Type 2 Diabetes Mellitus: Drug Interactions with Metformin

ASSESSMENT OF THE POTENTIAL AMELIORATING EFFECTS OF BM-MSCsOR INSULIN ON THE ALTERED METABOLIC STATUS OF PANCREAS, LIVER AND KIDNEYIN STZ-DIABETIC RATS.

Identification of Suitable Reference Genes for Quantitative Gene Expression Analysis in Innervated and Denervated Adipose Tissue from Cafeteria Diet‐Fed Rats

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