Myoglobin, creatine kinase MB, troponin T, and troponin I — rapid bedside assays in patients with acute chest pain

Abstract

Three new rapid, qualitative bedside immunoassays were evaluated in the diagnosis of patients with acute chest pain. The subjects, 122 patients in group 1 (bedside tests for myoglobin, creatine kinase MB) and 233 patients in group 2 (bedside tests for troponin I and sensitive troponin T) were admitted to hospital with acute chest pain for less than 12 h. The bedside tests were performed on admission, and 2, 4, and 6 h later. The correlation between the two parts of the rapid creatine kinase MB/myoglobin test during the first 12 h after the onset of chest pain was moderate in all patients (kappa=0.401, 95% confidence interval 0.321-0.483). The highest correlation was seen with the patients with definite and probable myocardial infarction. The correlations were smaller but significant also in other diagnostic groups (unstable angina pectoris, prolonged chest pain, and non-cardiac chest pain). The correlation between the rapid sensitive test for troponin T and rapid test for troponin I was significant in all groups (kappa=0.776, 95% confidence interval 0.711-0.841). The myoglobin part of the rapid creatine kinase MB/myoglobin test may be too non-specific for clinical diagnostic purposes [in non-infarct patients the myoglobin part was significantly more often positive than creatine kinase MB or troponin tests (P<0.001)].

Surface plasmon resonance immunosensor for human cardiac troponin T based on self-assembled monolayer

“…tion of cardiac troponin T might also be useful prognosticator in high risk patients whose standard evaluation of myocardial ischemia is not always accurate [3,4]. Several methods have been shown to be able to detect the troponin T such as enzymelinked immunosorbent assay (ELISA) [5], radioimmunoassay (RIA) [6] and immunochromatographic [7] tests. The first two are more usually used; however, they involve several steps and time consuming, whereas the second is a qualitative test.…”

High performance immunochromatographic assay for simultaneous quantitative detection of multiplex cardiac markers based on magnetic nanobeads

“…In the expected threshold value of these three markers (0.5 ng/mL for cTnI, 5 ng/mL for CKMB, and 50 ng/mL for Myo), visible bands appeared at the test lines, which indicated that the MICT performed well even without the MIR equipment. The LOD by naked eye was 0.5 ng/mL for cTnI, 5 ng/mL for CKMB, and 5 ng/mL for Myo 23.…”

Rapid electrical immunoassay of the cardiac biomarker troponin I through dielectrophoretic concentration using imbedded electrodes

“…The methods used for the diagnosis of AMI include conventional enzyme-linked immunosorbent assays (Wang et al, 2001), fluoroimmunoassays (Hayes et al, 2009), immunochromatographic tests (Penttil et al, 1999), spectrophotometric methods (Shiomi et al, 2005), electrochemiluminescence assays (Shen et al, 2011), and radioimmunoassays (Apple et al, 1997;Carraro et al, 1994). However, these approaches require multistep processing of samples, long diagnostic times, high costs, and trained personnel.…”