Myogenic cell transplantation improves in vivo regional performance in infarcted rabbit myocardium

Atkins, B. Zane; Hueman, Matthew T.; Meuchel, Jennifer M.; Cottman, Maisha J.; Hutcheson, Kelley A.; Taylor, Doris A.

doi:10.1016/s1053-2498(99)00096-0

Cited by 113 publications

(79 citation statements)

References 21 publications

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“…These graft kinetics may be totally compatible with the concept of passive contraction that was described by Taylor and colleagues as 'respond to stretch'. 8,9 In contrast, there were no papers found which described dimensional improvement of the left ventricle after myoblast transplantation as shown in group I, although attenuation of LV remodeling has been reported. 14,15 Because group I had about 10 times as many cells transplanted as those conventionally applied, we may well ascribe the reverse LV remodeling in that group to the large number of donor SM.…”

Section: Discussionmentioning

confidence: 94%

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Among various types of cell sources, skeletal myoblasts (SM) have drawn our attentions because of their clinical applicability and regenerative capacity. 6 -15 To date, several clinical trials of skeletal myoblast transplantation are under way in patients with chronically ischemic hearts that are not suitable for standard myocardial revascularization.…”

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confidence: 99%

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Transplanted Skeletal Myoblasts Can Fully Replace the Infarcted Myocardium When They Survive in the Host in Large Numbers

Tambara

Sakakibara²,

Sakaguchi³

et al. 2003

Circulation

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Background-It is not clear how many skeletal myoblsts (SM) can survive and exert beneficial effects in the host myocardial infarction (MI) area. We assessed the hypothesis that a large number of SM can replace the MI area with reverse left ventricular (LV) remodeling. Methods and Results-MI was created by left coronary artery ligation in male Lewis rats. Four weeks after ligation, 45 rats had skeletal myoblast transplantation in the MI area. They were randomized into 3 groups according to the number of SM: group I (nϭ15), 5ϫ10 7 ; group II (nϭ15), 5ϫ10 6 ; and group III (nϭ15), 5ϫ10 5 cells. Donor SM were obtained from neonatal Lewis rats and directly used without expansion. Another four weeks later, all rats were sacrificed following hemodynamic assessment. All heart sections were stained with anti-fast skeletal myosin heavy chain (FSMHC) antibody to determine the spacial extent of donor myocytes. Results-Four weeks after transplantation, LV diastolic dimension was decreased, fractional area change was increased, and MI size was decreased maximally in group I. Histological study showed that donor cells positive for FSMHC occupied the MI area with nearly normal wall thickness in group I, in which estimated volume of donor-derived muscle tissue was 40 mm 3 . In the other groups, FSMHC-positive cells were found only partly in the MI area.

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Transplanted Skeletal Myoblasts Can Fully Replace the Infarcted Myocardium When They Survive in the Host in Large Numbers

Tambara

Sakakibara²,

Sakaguchi³

et al. 2003

Circulation

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“…[13][14][15][16][17] Notably, numerous research groups have found that skeletal muscle-derived cells can successfully engraft in the heart and improve cardiac performance in animal models. [18][19][20][21][22][23][24][25][26][27][28][29][30] Currently, autologous skeletal myoblasts are being used in clinical trials for cardiac repair. [31][32][33][34][35] Our research group has isolated populations of murine skeletal muscle-derived stem cells (MDSCs) by using a modified preplate technique.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of dystrophin-expressing myocytes in the mdx heart by skeletal muscle stem cells

et al. 2005

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“…196: 70-78, 2003. Several pre-clinical (Atkins et al, 1999;Hutcheson et al, 2000;Pouzet et al, 2000;Scorsin et al, 2000;Jain et al, 2001) and clinical (Menasche et al, 2001) studies have shown promise for the therapeutic application of autologous human skeletal muscle cells (HuSkMC) to mitigate the deterioration of cardiac function resulting from myocardial infarction. Cells released from skeletal muscle biopsies were propagated in vitro in these studies and injected into damaged heart tissue.…”

mentioning

confidence: 99%

Characterization of proliferating human skeletal muscle‐derived cells in vitro: Differential modulation of myoblast markers by TGF‐β2

Stewart¹,

Masi²,

Cumming³

et al. 2003

Journal Cellular Physiology

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Adult human skeletal muscle-derived cells (HuSkMC) propagated in vitro are under investigation as a cell-based therapy for the treatment of myocardial infarction. We have characterized HuSkMC with respect to cell identity and state of differentiation as a prerequisite to their clinical use. Flow cytometric analysis of propagated HuSkMC revealed a population of cells that expressed the myoblast markers CD56 and desmin. The presence of myoblasts in these cultures was further confirmed by their capacity to form myotubes and increase creatine kinase activity when cultured in low serum conditions. The non-myoblast fraction of these propagated cells expressed TE7, a marker associated with the fibroblast phenotype. Spontaneous differentiation of myoblasts occurred during serial propagation of HuSkMC, as judged by myotube formation, thereby reducing the myoblast representative fraction with continued cell expansion. We examined transforming growth factor b2 (TGF-b2) for its utility in controlling this spontaneous differentiation of adult human myoblasts in vitro. Propagation of HuSkMC in the presence of 1 ng/ml TGFb2 for 5 days decreased desmin expression within the myoblast population and caused a parallel reduction of creatine kinase activity. CD56 expression was unaffected, indicating a differential regulation of these myoblast markers. The reduction in desmin expression and creatine kinase activity was, however, reversible upon the removal of TGF-b. These data collectively indicate that TGF-b2 restrained differentiation of adult human skeletal myoblasts during propagation without causing irreversible loss of the myoblast phenotype, demonstrating the potential utility of using TGF-b2 during cultivation and expansion of HuSkMC intended for therapeutic implantation.

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Myogenic cell transplantation improves in vivo regional performance in infarcted rabbit myocardium

Cited by 113 publications

References 21 publications

Transplanted Skeletal Myoblasts Can Fully Replace the Infarcted Myocardium When They Survive in the Host in Large Numbers

Transplanted Skeletal Myoblasts Can Fully Replace the Infarcted Myocardium When They Survive in the Host in Large Numbers

Regeneration of dystrophin-expressing myocytes in the mdx heart by skeletal muscle stem cells

Characterization of proliferating human skeletal muscle‐derived cells in vitro: Differential modulation of myoblast markers by TGF‐β2

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