Myofibroblasts and Interactions with Other Cells: Contribution of the Tissue Engineering

Moulin, Véronique; Bellemare, Judith; Bergeron, Danielle; Genest, Hervé; Roy, Michel; Lopez-Vallé, Carlos A.

doi:10.1007/978-3-642-22697-7_9

Cited by 1 publication

(2 citation statements)

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“…Wmyos are characterized by their expression of α-SMA, which is a recognized marker of these cells (Hinz et al 2007). They play a central role during wound healing by contributing to ECM formation and wound contraction (Hinz 2016;Moulin et al 2012Moulin et al , 1998Moulin et al , 1999. Their actions in the different wound healing mechanisms are well studied but the means by which they can communicate with other cells remain poorly known.…”

Section: Discussionmentioning

confidence: 99%

“…Wound myofibroblasts (Wmyos) are cells that appear during this phase and differentiate mainly from fibroblasts (Desmouliere et al 1993; Hinz 2016) triggered by environmental factors such as inflammatory cell‐secreted transforming growth factor‐ß (TGFß1) (Postlethwaite et al 2004). Wmyos have morphological and biochemical characteristics of both fibroblasts and smooth muscle cells (Véronique Moulin et al 2012). They are characterized by a heightened expression of alpha‐Smooth Muscle Actin (α‐SMA), which is a recognized marker of these cells (Hinz et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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PLGF-1 contained in normal wound myofibroblast-derived microvesicles stimulated collagen production by dermal fibroblasts

Arif

Larochelle

Moulin

2020

J. Cell Commun. Signal.

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During the last stages of wound healing, myofibroblasts differentiate mainly from fibroblasts. Myofibroblasts from normal skin wounds (Wmyo) can communicate with its surrounding using secreted factors. They also have the capacity to produce microvesicles (MVs), a type of extracellular vesicles, as mediators of intercellular communication. MVs cargo are potentially capable of regulating the behavior of targeted cells and tissues. The aim of this study is to evaluate the effect of Wmyo‐derived MVs on dermal fibroblasts and to determine the responsible signaling molecule. Microvesicles were obtained from culture media of myofibroblasts and characterized using protein quantification, dynamic light scattering and transmission electron microscopy. Uptake of fluorescent MVs in fibroblasts was assessed by flow cytometry. Cytokines concentrations were quantified in MV samples by a multiplex ELISA. Different concentration of MVs or a selected cytokine were used as treatments over fibroblasts culture for 5 days. Following the treatments, parameters linked to the extracellular matrix were studied. Lastly, the selected cytokine was neutralized within MVs before evaluating collagen production. We showed that Wmyo derived‐MVs were internalized by dermal fibroblasts. Cytokine array analysis revealed that a large amount of placental growth factor 1 (PLGF‐1) (0.88 ± 0.63 pg/μg proteins in MVs) could be detected in MVs samples. Cutaneous fibroblasts treated with MVs or PLGF‐1 showed significantly stimulated procollagen I level production (Fold change of 1.80 ± 0.18 and 2.07 ± 0.18, respectively). Finally, the neutralization of PLGF‐1 in MVs significantly inhibited the production of procollagen I by fibroblasts. Our study shows that Wmyo derived‐MVs are involved in intercellular communication by stimulating collagen production by fibroblasts during wound healing. This effect is possibly attained through PLGF‐1 signalling. These findings represent a promising opportunity to gain insight into how MVs and Wmyo may mediate the healing of a skin wound.

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