Myofibroblast Expression in Skin Wounds Is Enhanced by Collagen III Suppression

Al-Qattan, M. M.; Abd-Elwahed, Mervat M.; Hawary, Khalid; Arafah, Maha; Shier, Medhat K.

doi:10.1155/2015/958695

Cited by 18 publications

(19 citation statements)

References 17 publications

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“…Col I and Col III are metabolites of MFB, and are also valid markers for MFB. 24,25 We found that treatment of Jag1 siRNA significantly enhanced the expression of a-SMA, Collagen I, and Collagen III compared with the control group (P < 0.05; Fig. 2B, C, and D).…”

Section: Jagged 1 Inhibited the Differentiation Of Escs To Mfb In Vitromentioning

confidence: 71%

Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells

et al. 2018

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Skin wound healing involves Notch/Jagged1 signaling. However, little is known how Jag1 expression level in epidermal stem cells (ESCs) contributes to wound healing and scar formation. We applied multiple cellular and molecular techniques to examine how Jag1 expression in ESCs modulates ESCs differentiation to myofibroblasts (MFB) in vitro, interpret how Jag1 expression in ESCs is involved in wound healing and scar formation in mice, and evaluate the effects of porcine acellular dermal matrix (ADM) treatment on wound healing and scar formation. We found that Jag1, Notch1 and Hes1 expression was up-regulated in the wound tissue during the period of wound healing. Furthermore, Jag1 expression level in the ESCs was positively associated with the level of differentiation to MFB. ESC-specific knockout of Jag1 delayed wound healing and promoted scar formation in vivo. In addition, we reported that porcine ADM treatment after skin incision could accelerate wound closure and reduce scar formation in vivo. This effect was associated with decreased expression of MFB markers, including α-SMA Col-1 and Col-III in wound tissues. Finally, we confirmed that porcine ADM treatment could increase Jag1, Notch1 and Hesl expression in wound tissues. Taken together, our results suggested that ESC-specific Jag1 expression levels are critical for wound healing and scar formation, and porcine ADM treatment would be beneficial in promoting wound healing and preventing scar formation by enhancing Notch/Jagged1 signaling pathway in ESCs.

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Section: Jagged 1 Inhibited the Differentiation Of Escs To Mfb In Vitromentioning

confidence: 71%

Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells

et al. 2018

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“…Col I and Col III are metabolites of MFB, and are also valid markers for MFB [35,36]. We found that the expression of α-SMA, Col I, and Col III was significantly lower after anti-miR-203 treatment compared with Cellular Physiology and Biochemistry Cellular Physiology and Biochemistry control (P < 0.05; Fig.…”

Section: Mir-203 Promoted the Formation Of Mfb In Vitromentioning

confidence: 80%

microRNA-203 Modulates Wound Healing and Scar Formation via Suppressing Hes1 Expression in Epidermal Stem Cells

Zhou

Shu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Little is known how miR-203 is involved in epidermal stem cells (ESCs) differentiation and scar formation. Methods: We first used luciferase assay to determine the interaction of miR-203 with the 3’-UTR in regulation of Hes1 expression. We then used flow cytometry to analyze the effects of miR-203 expression on the differentiation of ESCs to MFB by determination of CK15 ratio and α-SMA. To confirm the results of flow cytometry analysis, we used Western blot to examine the expression of α-SMA, Collagen I (Col I), and Collagen III (Col III), as well as the expression of Notch1, Jagged1, and Hes1 in ESCs after the treatment of pre-miR-203 or anti-miR-203. Finally, we examined the effects local anti-miR-203 treatment on would closure and scar formation using a mouse skin wound model. Results: Pre-miR-203 treatment increased ESCs differentiation to MFB cells, as indicated by decreased CK15 ratio and increased MFB biomarkers. This phenomenon was reversed by overexpression of Hes1 in ESCs. In addition, skin incision increased expression of miR-203 in wound tissue. Local treatment of anti-miR-203 could accelerate wound closure and reduce scar formation in vivo, which was associated with increased re-epithelialization, skin attachment regeneration, and collagen reassignment. Finally, we confirmed that anti-miR-203 treatment could inhibit ESCs differentiation in vivo via increasing Hesl expression. Conclusion: Taken together, our results suggested that overexpression of miR-203 in ESCs after skin wound may be a critical mechanism underlying the scar formation.

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“…Her symptoms included joint hypermobility affecting large and small joints, arthralgia, soft "velvety" skin as well as vasomotor instability exemplified by orthostatic hypotension. In Ehlers-Danlos vascular type, collagen III is decreased [9]. Al-Qattan et al proposed a paradoxical increase in myofibroblast expression when collagen III expression is decreased [9].…”

Section: Discussionmentioning

confidence: 99%

“…In Ehlers-Danlos vascular type, collagen III is decreased [9]. Al-Qattan et al proposed a paradoxical increase in myofibroblast expression when collagen III expression is decreased [9]. Compared to the other types of EDS, vascular type EDS is known to develop postoperative scar hypertrophy and spontaneous skin keloid-like lesions [10,11].…”

Section: Discussionmentioning

confidence: 99%

Ehlers-Danlos Syndrome Presenting as Airway Obstruction and Hoarseness

Jessica¹,

Seebald²,

Joseph³

et al. 2015

J Case Rep Med

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Ehlers-Danlos syndrome is an autosomal dominant genetic defect in connective tissue synthesis and structure. The genetic disorder is classified into six major types based on clinical signs and symptoms as well as genetic testing. We describe the case of a 42-year-old female who presents with dyspnea and hoarseness. After an anterior glottic web was discovered, the attempts to remove the web created excess scarring. She was referred to a geneticist and diagnosed with classic Ehlers-Danlos syndrome and showed signs of vascular type. Genetic disorders should be considered in differentials when poor healing or abnormal scarring occurs post surgery.

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Myofibroblast Expression in Skin Wounds Is Enhanced by Collagen III Suppression

Cited by 18 publications

References 17 publications

Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells

Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells

microRNA-203 Modulates Wound Healing and Scar Formation via Suppressing Hes1 Expression in Epidermal Stem Cells

Ehlers-Danlos Syndrome Presenting as Airway Obstruction and Hoarseness

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