Myocardial substrate changes in advanced ischaemic and advanced dilated human heart failure

Cao, Jacob; Koay, Yen Chin; Quek, Lake‐Ee; Parker, Benjamin L.; Lal, Sean; O’Sullivan, John

doi:10.1002/ejhf.1479

Cited by 7 publications

(9 citation statements)

References 8 publications

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“…These samples are not post-mortem. Histological analysis of the donor samples were shown to be structurally normal as per formal pathological examination 2,3 . LV samples from both donors and heart failure patients were collected and and snap frozen (−196°C) immediately within the operating theatre.…”

Section: Methodsmentioning

confidence: 97%

“…Human heart tissue. Donor hearts were procured but not used for heart transplantation (reasons included transportation logistics, donor-recipient mismatch in size, and immune incompatibility), whilst heart failure samples comprised those patients undergoing heart transplantation; both cohorts as previously described 1,2,[4][5][6][7][8]71,72 . These samples are not post-mortem.…”

Section: Methodsmentioning

confidence: 99%

“…The paucity of human left ventricular myocardial tissue for research is a major limitation to directly studying heart failure pathogenesis. Our extensive and heart bank has been carefully procured over the last 30 years, and novel findings in our have been replicated by numerous and unrelated groups, and cross-validated with samples derived elsewhere and in model systems [1][2][3][4][5][6][7][8] .…”

mentioning

confidence: 93%

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Core functional nodes and sex-specific pathways in human ischaemic and dilated cardiomyopathy

Parker

Pearson

et al. 2020

Nat Commun

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Poor access to human left ventricular myocardium is a significant limitation in the study of heart failure (HF). Here, we utilise a carefully procured large human heart biobank of cryopreserved left ventricular myocardium to obtain direct molecular insights into ischaemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM), the most common causes of HF worldwide. We perform unbiased, deep proteomic and metabolomic analyses of 51 left ventricular (LV) samples from 44 cryopreserved human ICM and DCM hearts, compared to age-, gender-, and BMI-matched, histopathologically normal, donor controls. We report a dramatic reduction in serum amyloid A1 protein in ICM hearts, perturbed thyroid hormone signalling pathways and significant reductions in oxidoreductase co-factor riboflavin-5monophosphate and glycolytic intermediate fructose-6-phosphate in both; unveil genderspecific changes in HF, including nitric oxide-related arginine metabolism, mitochondrial substrates, and X chromosome-linked protein and metabolite changes; and provide an interactive online application as a publicly-available resource.

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Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 93%

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Core functional nodes and sex-specific pathways in human ischaemic and dilated cardiomyopathy

Parker

Pearson

et al. 2020

Nat Commun

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Section: Introductionmentioning

confidence: 99%

“…Cardiovascular biobanking represents a disease‐oriented biobank, providing unique opportunities to utilize cardiac and vascular samples for translational research into heart failure, valvular, and aortic diseases. 7 , 8 , 9 , 10 , 11 Current techniques for diagnosis, classification, and treatment of cardiovascular diseases rely primarily on interpretation of history and physical signs, imaging, and blood biomarkers. Further research into cardiovascular disease pathophysiology has been limited by a relative lack of access to quality human cardiac and aortic tissue, and the inherent shortcomings of most animal models of heart disease.…”

Section: Introductionmentioning

confidence: 99%

Models of cardiovascular surgery biobanking to facilitate translational research and precision medicine

et al. 2021

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Biobanking in health care has evolved over the last few decades from simple biological sample repositories to complex and dynamic units with multi‐organizational infrastructure networks and has become an essential tool for modern medical research. Cardiovascular tissue biobanking provides a unique opportunity to utilize cardiac and vascular samples for translational research into heart failure and other related pathologies. Current techniques for diagnosis, classification, and treatment monitoring of cardiac disease relies primarily on interpretation of clinical signs, imaging, and blood biomarkers. Further research at the disease source (i.e. myocardium and blood vessels) has been limited by a relative lack of access to quality human cardiac tissue and the inherent shortcomings of most animal models of heart disease. In this review, we describe a model for cardiovascular tissue biobanking and databasing, and its potential to facilitate basic and translational research. We share techniques to procure endocardial samples from patients with hypertrophic cardiomyopathy, heart failure with reduced ejection fraction, and heart failure with preserved ejection fraction, in addition to aortic disease samples. We discuss some of the issues with respect to data collection, privacy, biobank consent, and the governance of tissue biobanking. The development of tissue biobanks as described here has significant scope to improve and facilitate translational research in multi‐omic fields such as genomics, transcriptomics, proteomics, and metabolomics. This research heralds an era of precision medicine, in which patients with cardiovascular pathology can be provided with optimized and personalized medical care for the treatment of their individual phenotype.

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Network-based identification of diagnosis-specific trans-omic biomarkers via integration of multiple omics data

Rashid,

Hamano,

Iida

et al. 2024

Biosystems

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Myocardial substrate changes in advanced ischaemic and advanced dilated human heart failure

Cited by 7 publications

References 8 publications

Core functional nodes and sex-specific pathways in human ischaemic and dilated cardiomyopathy

Core functional nodes and sex-specific pathways in human ischaemic and dilated cardiomyopathy

Models of cardiovascular surgery biobanking to facilitate translational research and precision medicine

Network-based identification of diagnosis-specific trans-omic biomarkers via integration of multiple omics data

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