Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils

Sahraoui, Abdelhamid; Dewachter, Céline; Medina, Geoffrey de; Naeije, Robert; Aouichat-Bouguerra, S.; Dewachter, Laurence

doi:10.1371/journal.pone.0148117

Cited by 42 publications

(45 citation statements)

References 58 publications

(58 reference statements)

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“…In animal models, obese and diabetic rats show an increase in expression of CACNA1C in myocardial tissue. [35][36][37][38] PDE1A is a Ca(2C)/calmodulin-dependent phosphodiesterase that is activated by calmodulin in the presence of Ca(2C), 39 and its regulation has an important role in neointima formation in atherosclerosis and restenosis. 40 Moreover, lifestyles have an impact on the circadian rhythm which could in turn modify behavior, metabolism, and weight control.…”

Section: Discussionmentioning

confidence: 99%

DNA methylation and obesity traits: An epigenome-wide association study. The REGICOR study

et al. 2017

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Obesity is associated with increased risk of several diseases and has become epidemic. Obesity is highly heritable but the genetic variants identified by genome-wide association studies explain only limited variability. Epigenetics could contribute to explain the missing variability. The study aim was to discover differential methylation patterns related to obesity. We designed an epigenome-wide association study with a discovery phase in a subsample of 641 REGICOR study participants, validated by analysis of 2,515 participants in the Framingham Offspring Study. Blood DNA methylation was assessed using Illumina HumanMethylation450 BeadChip. Next, we meta-analyzed the data using the fixed effects method and performed a functional and pathway analysis using the Ingenuity Pathway Analysis software. We were able to validate 94 CpGs associated with body mass index (BMI) and 49 CpGs associated with waist circumference, located in 95 loci. In addition, we newly discovered 70 CpGs associated with BMI and 33 CpGs related to waist circumference. These CpGs explained 25.94% and 29.22% of the variability of BMI and waist circumference, respectively, in the REGICOR sample. We also evaluated 65 of the 95 validated loci in the GIANT genome-wide association data; 10 of them had Tag SNPs associated with BMI. The top-ranked diseases and functions identified in the functional and pathway analysis were neurologic, psychological, endocrine, and metabolic.

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Section: Discussionmentioning

confidence: 99%

DNA methylation and obesity traits: An epigenome-wide association study. The REGICOR study

et al. 2017

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“…Disturbance of myocardial lipid metabolism may induce stress in sarcoplasmic reticula, Ca ++ transport perturbation (as we obtain in MHFD), resulting in myocardial dysfunction and heart failure [ 101 ]. Different pathways are involved in myocardial function alterations, such as PKA, caMK2D [ 102 ], and PIK fyve, via mitochondrial damage [ 103 ].…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

Sibouakaz

Othmani-Mecif

Fernane

et al. 2018

Analytical Cellular Pathology

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Early weight gain induced by high-fat diet has been identified as a predictor for cardiac disease, one of the most serious public health problems. Our goal is to study the influence of a HFD on biochemical, oxidant stress parameters, and the cardiac ultrastructure in both male and female prepubertal models. Experiments were carried on 24 prepubertal New Zealand white rabbits, randomly assigned to male and female control (MC and FC, resp.) or HFD (MHFD and FHFD, resp.) groups (n = 6) for 3 months. Body and heart weights and some biochemical and oxidative stress parameters such as lipids, calcium, CKMB, MDA, uric acid, ascorbic acid, and AOA are evaluated in plasma and the left ventricle. Under HFD effect, plasma parameters, such as lipids (TL, PL, and LDL-C), MDA, and CK-MB, increase more significantly in male than in female groups, when AA decreases. Some cardiac parameters such as TG and UA increase, when AA and AOA decrease; these variations are more significant in FHFD. In both male and female rabbits, HFD caused changes in heart ultrastructure, junctional complexes, mitochondria size and form, and so on. Early HFD feeding induced overweight, oxidative stress, and metabolic alterations in plasma and the heart of prepubertal rabbits, whereas lipotoxicity has especially a negative impact on male plasma but affects more the female heart ultrastructure.

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“…Previous reports, including ours, have shed light on pronounced physiological disorders in P . obesus after hypercaloric diet feeding [ 31 , 32 , 18 ]. We further extend these observations herein, showing that 24-week HED fed P .…”

Section: Discussionmentioning

confidence: 99%

The desert gerbil Psammomys obesus as a model for metformin-sensitive nutritional type 2 diabetes to protect hepatocellular metabolic damage: Impact of mitochondrial redox state

Gouaref¹,

Détaille²,

Wiernsperger³

et al. 2017

PLoS ONE

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IntroductionWhile metformin (MET) is the most widely prescribed antidiabetic drug worldwide, its beneficial effects in Psammomys obesus (P. obesus), a rodent model that mimics most of the metabolic features of human diabetes, have not been explored thoroughly. Here, we sought to investigate whether MET might improve insulin sensitivity, glucose homeostasis, lipid profile as well as cellular redox and energy balance in P. obesus maintained on a high energy diet (HED).Materials and methodsP. obesus gerbils were randomly assigned to receive either a natural diet (ND) consisting of halophytic plants (control group) or a HED (diabetic group) for a period of 24 weeks. MET (50 mg/kg per os) was administered in both animal groups after 12 weeks of feeding, i.e., the time required for the manifestation of insulin resistance in P. obesus fed a HED. Parallel in vitro experiments were conducted on isolated hepatocytes that were shortly incubated (30 min) with MET and energetic substrates (lactate + pyruvate or alanine, in the presence of octanoate).ResultsIn vivo, MET lowered glycemia, glycosylated haemoglobin, circulating insulin and fatty acid levels in diabetic P. obesus. It also largely reversed HED-induced hepatic lipid alterations. In vitro, MET increased glycolysis but decreased both gluconeogenesis and ketogenesis in the presence of glucogenic precursors and medium-chain fatty acid. Importantly, these changes were associated with an increase in cytosolic and mitochondrial redox states along with a decline in respiration capacity.ConclusionsMET prevents the progression of insulin resistance in diabetes-prone P. obesus, possibly through a tight control of gluconeogenesis and fatty acid β-oxidation depending upon mitochondrial function. While the latter is increasingly becoming a therapeutic issue in diabetes, the gut microbiota is another promising target that would need to be considered as well.

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Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils

Cited by 42 publications

References 58 publications

DNA methylation and obesity traits: An epigenome-wide association study. The REGICOR study

DNA methylation and obesity traits: An epigenome-wide association study. The REGICOR study

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

The desert gerbil Psammomys obesus as a model for metformin-sensitive nutritional type 2 diabetes to protect hepatocellular metabolic damage: Impact of mitochondrial redox state

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