Myocardial protective effect of Sheng Mai San (SMS) and a lignan-enriched extract of Fructus Schisandrae, in vivo and ex vivo

Li, Pui Chun; Mak, Duncan H.; Poon, Michel Kwong Tat; Ip, Siu‐Po; Ko, Kam Ming

doi:10.1016/s0944-7113(96)80039-0

Cited by 38 publications

(33 citation statements)

References 9 publications

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“…The aorta was quickly cannulated and the isolated heart was transferred to the warm and moistured chamber of the perfusion apparatus. The heart was perfused retrogradely according to the Langendorff method as described (Li et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Dang‐Gui Buxue Tang produces a more potent cardioprotective effect than its component herb extracts and enhances glutathione status in rat heart mitochondria and erythrocytes

Mak

Chiu

Dong

et al. 2006

Phytotherapy Research

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The effects of pretreatment with Dang-Gui Buxue Tang (DBT, a decoction of Astragali and Angelica roots) and its component herb extracts on myocardial ischaemia-reperfusion (IR) injury were examined in rats ex vivo. DBT and its component herb extracts could protect against myocardial IR injury in a dose-dependent manner. The more potent cardioprotection afforded by DBT pretreatment than that of a mixture of Astragali and Angelica root extracts was associated with a much higher extraction yield of active ingredients from Angelica root in the herbal decoction. The high level of active ingredients might increase their bioavailability after oral administration. DBT pretreatment could enhance myocardial mitochondrial as well as red blood cell (RBC) glutathione status, thereby increasing their resistance to oxidative stress-induced injury in rats. The measurement of RBC glutathione status may serve as a useful index for the antioxidant effect produced by DBT treatment in human subjects.

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Section: Introductionmentioning

confidence: 99%

Dang‐Gui Buxue Tang produces a more potent cardioprotective effect than its component herb extracts and enhances glutathione status in rat heart mitochondria and erythrocytes

Mak

Chiu

Dong

et al. 2006

Phytotherapy Research

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“…100%) was estimated as an index of cellular injury. LDH activity was measured in the media and cell lysates prepared from unchallenged/challenged cells, with or without drug pretreatment, as described by Li et al [13] .…”

Section: Assessment Of Cellular Injurymentioning

confidence: 99%

Effects of Schisandrin B Enantiomers on Cellular Glutathione and Menadione Toxicity in AML12 Hepatocytes

Chiu

Leung²,

Poon³

et al. 2006

Pharmacology

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Effects of schisandrin B enantiomer ((+)Sch B and (–)Sch B) treatment on the reduced cellular glutathione (GSH) level and susceptibility to menadione-induced toxicity were investigated and compared in AML12 hepatocytes. (+)Sch B or (–)Sch B treatment at 6.25 µmol/l produced a time-dependent change in cellular GSH level, with the maximal stimulation occurring 16 h after dosing. (+)Sch B/(–)Sch B pretreatment for 16 h dose-dependently protected against menadione toxicity, with the maximum degree of protection observable at 6.25 µmol/l and the extent of protection afforded by (–)Sch B being larger than that of (+)Sch B. The cytoprotection was associated with a parallel enhancement in cellular GSH level in both non-menadione (control) and menadione-intoxicated cells. While the GSH depletion produced by buthionine sulfoximine/phorone treatment largely abrogated the cytoprotective action of (+)Sch B/(–)Sch B, it almost completely abolished the GSH-enhancing effect of (+)Sch B and (–)Sch B in both control and menadione-treated cells. Both (+)Sch B and (–)Sch B treatments increased the GSH reductase activity in control and menadione-treated cells, with the stimulatory action of (–)Sch B being more potent than that of (+)Sch B in the control condition. (+)Sch B and (–)Sch B also enhanced the γ-glutamate cysteine ligase activity in menadione-intoxicated cells. The results indicate that (–)Sch B is more effective than (+)Sch B in enhancing cellular GSH and protecting against oxidant injury in hepatocytes.

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“…The heart was retrogradely perfused according to the Langendorff method as described in ref. 21. The apex of the heart was attached via a metal hook to an unextendable cotton thread that was connected to a force displacement transducer (Model FT03; Grass, Quincy, MA, USA), and the isometric contractions of the heart were recorded on a polygraph (Model 7-8P, Grass).…”

Section: Isolated-perfused Rat Heartmentioning

confidence: 99%

“…LDH activity in all fractions of coronary effluent was assayed as described in ref. 21. The extent of LDH leakage was estimated by computing the area under the curve of the graph plotting the percent LDH activity (with respect to the mean preischemic value measured during the equilibration period) against the reperfusion time (1 -20 or 40 min) as described in ref.…”

Section: Myocardial I-rmentioning

confidence: 99%

Altered Susceptibility to Ischemia-Reperfusion Injury in Isolated-Perfused Hearts of Short-Term Diabetic Rats Associated With Changes in Non-enzymatic Antioxidants

Mak

Poon

et al. 2001

Japanese Journal of Pharmacology

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ABSTRACT-The effects of short-term (2-week) diabetes on myocardial ischemia-reperfusion (I-R) injury and associated changes in myocardial non-enzymatic antioxidant level were examined. Isolated-perfused hearts prepared from control and diabetic rats were subjected to increasing periods of ischemia and reperfusion, and myocardial I-R injury was assessed by measuring the extent of lactate dehydrogenase (LDH) leakage and contractile force recovery. While a brief period (20 min) of post-ischemic reperfusion caused a smaller extent of LDH leakage, the prolonged period (40 min) of reperfusion produced a greater degree of I-R injury in diabetic hearts, as indicated by the impaired recovery of contractile force. The apparent protection against I-R injury in diabetic hearts during the early phase of post-ischemic reperfusion was associated with increases in myocardial reduced glutathione/ascorbic acid and =-tocopherol levels, with the effect on = -tocopherol being most prominent. Insulin treatment could reverse the diabetes-associated changes in susceptibility to myocardial I-R injury and antioxidant response. The ensemble of results indicates that the myocardium isolated from short-term diabetic rat can produce a beneficial antioxidant response to I-R challenge, which may, in turn, be attributable to the decreased susceptibility to I-R injury observable during the early phase of reperfusion.

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Myocardial protective effect of Sheng Mai San (SMS) and a lignan-enriched extract of Fructus Schisandrae, in vivo and ex vivo

Cited by 38 publications

References 9 publications

Dang‐Gui Buxue Tang produces a more potent cardioprotective effect than its component herb extracts and enhances glutathione status in rat heart mitochondria and erythrocytes

Dang‐Gui Buxue Tang produces a more potent cardioprotective effect than its component herb extracts and enhances glutathione status in rat heart mitochondria and erythrocytes

Effects of Schisandrin B Enantiomers on Cellular Glutathione and Menadione Toxicity in AML12 Hepatocytes

Altered Susceptibility to Ischemia-Reperfusion Injury in Isolated-Perfused Hearts of Short-Term Diabetic Rats Associated With Changes in Non-enzymatic Antioxidants

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