Myocardial oxygen consumption of blood-perfused, isolated, supported, rat heart

Gamble, W. J.; Conn, PA; Kumar, Avnish; Plenge, Robert M.; Monroe, RG

doi:10.1152/ajplegacy.1970.219.3.604

Cited by 48 publications

(11 citation statements)

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“…When extrapolating these values to the heart in situ, it must be re called that the rat heart beats at about 300 beats/min. The oxygen up take per beat is 0.6-1 /d/min/g fresh tissue whether perfused with KrebsHenseleit bicarbonate or with blood [22,41,49]. The higher value for oxygen uptake for the rat heart corresponds to 7 ml/min/100 g for the heart in vivo, beating at 70/min, which is similar to the measured values found in the unanaesthetized dog.…”

Section: Possible Contribution Of Anaerobic Glycolysis To Energy Demasupporting

confidence: 81%

“…The ATP demand of the amphibian and reptilian heart is much less than that of the mammalian heart, as shown by a slower heart rate, a lower work output, and a lower oxygen uptake during aerobic conditions [7,8,63], On the basis of these differences, the ATP demand of the working reptilian or amphibian heart can be estimated at about V5 to of that of the isolated working mammalian heart [22,41]. The actual measured tissue activity of phosphofructokinase in turtle (Pseudemys scripta) and rat heart are similar: 14-20 /¿mole substrate transformed/min/g fresh tissue at 22-25 °C [55,58], Observations also show that the maximal rates of lactate production by the whole turtle heart and the rat heart during anaerobiosis are similar.…”

Section: Importance Of Glucose For the Oxidative Metabolism Of The Heartmentioning

confidence: 99%

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Substrate Utilization and Glycolysis in the Heart

Lh¹

1971

Cardiology

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Section: Possible Contribution Of Anaerobic Glycolysis To Energy Demasupporting

confidence: 81%

Section: Importance Of Glucose For the Oxidative Metabolism Of The Heartmentioning

confidence: 99%

Substrate Utilization and Glycolysis in the Heart

Lh¹

1971

Cardiology

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“…The mean time required for the removal of the heart and for initiating perfusion of the isolated heart was 815.7 ± 109 s, similar to literature data regarding similar experiments conducted on cats and rats (15)(16)(17).…”

Section: Support Animalsupporting

confidence: 86%

Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations

Júnior

Oliveira

Carmo

et al. 2003

Braz J Med Biol Res

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In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90 min. Parameters for measurement of ventricular performance using different indices measured directly or indirectly from intraventricular pressure were defined as: maximum peak pressure, final diastolic pressure, pressure developed, first derivative of maximum pressure (dP/dt max ), first derivative of minimum pressure (dP/dt min ), systolic stress of the left ventricle (s s ), and maximum elastance of the left ventricle. Isolated hearts subjected to regional and global ischemia presented significant worsening of all measured parameters. Less discriminative parameters were dP/dt max and dP/ dt min . Elastance was the most sensitive parameter during the reperfusion period, demonstrating an early loss of ventricular function during reperfusion. The model proved to be stable and reproducible and permitted the study of several variables in the isolated heart, such as ischemia and reperfusion phenomena, the effects of different drugs, surgical interventions, etc. The model introduces an advantage over the classical models which use crystalloid solutions as perfusate, because parabiotic circulation mimics heart surgery with extracorporeal circulation.

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“…Another factor (Gibbs, 1978) is the fraction of the basal metabolism needed for amino acid transport and protein synthesis and degradation (Chapman and Millward et al, 1975;Everett et al, 1977;Banos et al, 1978;Earl et al, 1978;Burns and Reddy, 1978;Schreiber et al, 1977). Gamble et al (1970) report an average value for total mV0 2 of 9.1 ml 0 2 /min per 100 g at 100 mm Hg left ventricular pressure which compares favorably with our value of 9.2 ml/min per 100 g; a lower range of values (4.9-8.6) was reported in unanesthetized resting dogs by Gregg et al (1965).…”

Section: Basal Metabolismmentioning

confidence: 99%

Oxygen consumption of the nonworking and potassium chloride-arrested dog heart.

Gibbs¹,

Papadoyannis²,

Drake³

et al. 1980

Circulation Research

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SUMMARY In 21 dogs on cardiopulmonary bypass with ventricles kept empty, the mean beating but nonworking myocardial oxygen consumption (mVO 2 ) was 3.8 ml/min per 100 g at a heart rate of 158 beats/min. After subsequent potassium chloride arrest, the basal mVOs was 1.74 ml/min per 100 g. To compare these values with the working situation, we measured mVOj in these hearts before instituting bypass when the heart rate averaged 179 beats/min and arterial pressure averaged 108 mm Hg; mVOj was 9.2 ml/min per 100 g. Atrioventricular dissociation was induced in five beating, nonworking hearts; electrical pacing at increasing heart rates produced a linear increase in mVO2. Extrapolation to zero heart rate yielded a value of 1.24 ml/min per 100 g, which was not significantly different from the KC1 arrest value of 1.25 ml/min per 100 g in these same hearts. THE BASAL OXYGEN consumption of cardiac tissue is higher than that of skeletal muscle. Different groups of experiments, using a variety of techniques to induce cardiac arrest, have measured the basal metabolism in several species of animals (for reviews, see Lochner et al., 1968;Gibbs, 1978). There is a fairly wide scatter among the different estimates, presumably as a result of technique and species differences. It is desirable to measure the basal metabolism accurately so that the real energy cost of a single cardiac contraction can be determined. To calculate this value, the basal metabolism must be subtracted from the total metabolism of the working heart.In the present investigation a cardiopulmonary bypass technique was used so that the oxygen consumption of arrested blood-perfused hearts could be followed in vivo for long periods of time (40 minutes and more). The per beat oxygen cost of (1) the beating working heart and (2) the beating nonworking heart was determined. We know of no other experiments on the in situ blood-perfused heart in which basal metabolism has been studied in this way.

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Myocardial oxygen consumption of blood-perfused, isolated, supported, rat heart

Cited by 48 publications

References 0 publications

Substrate Utilization and Glycolysis in the Heart

Substrate Utilization and Glycolysis in the Heart

Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations

Oxygen consumption of the nonworking and potassium chloride-arrested dog heart.

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