Myocardial lipidomics profiling delineate the toxicity of traditional Chinese medicine Aconiti Lateralis radix praeparata

Cai, YaMei; Gao, Yue; Tan, Guangguo; Wu, Si; Dong, Xin; Lou, Ziyang; Zhu, Zhenyu; Chai, Yifeng

doi:10.1016/j.jep.2013.03.017

Cited by 61 publications

(33 citation statements)

References 34 publications

(35 reference statements)

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“…It is widely applied in treatments for the cardiovascular system, such as heart failure, hypertension, and arrhythmia (Zhao et al, 2012;, and for anti-inflammation and analgesic action, such as in rheumatism and painful joints (Cai et al, 2013). Then there are other applications, including for anti-aging, protecting the kidney, as an anti-diabetic and for lipid-lowering, helping the immune system, and so on (Zhou et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Wang

Wan

Zhou

et al. 2017

J. Zhejiang Univ. Sci. B

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This study investigated the protective effect of the compatibility of hypaconitine (HA) and glycyrrhetinic acid (GA) on H9c2 cells under oxygen and glucose deprivation (OGD)-induced injury, and the possible mechanisms. We found that HA+GA significantly improved pathology and morphology of the nucleus and ultrastructure of H9c2 cells under OGD as determined by Hoechst 33342 staining and transmission electron microscopy (TEM) tests. It also reduced the releases of lactate dehydrogenase (LDH), creatine kinase-myocardial band isoenzyme (CK-MB), and aspartate transaminase (AST) from the cultured supernatant of H9c2 cells, which were tested by enzyme-linked immune sorbent assay (ELISA) kits. In addition, it lessened the apoptotic rate as determined by a fluorescein isothiocyanate-annexin V/propidium iodide (FITC-AV/PI) double staining assay. It was also found that HA+GA might regulate the protein expression associated with the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. Overall, the study demonstrated that HA+GA protected H9c2 cells against OGD-induced injury, and the signaling mechanism might be related to the PI3K/Akt signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Wang

Wan

Zhou

et al. 2017

J. Zhejiang Univ. Sci. B

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show abstract

“…The administration dose in the current study is in accordance with clinical use and had been confirmed to have no cardiotoxicity by our group. 45 For the control group ( n = 9), saline (0.4 mL) was injected in the same manner. At day 15, after hemodynamics assessments, the hearts of 3 rats in control and DOX groups, respectively, were excised for preparation of CMC columns.…”

Section: Experimental Sectionmentioning

confidence: 99%

Comparative Normal/Failing Rat Myocardium Cell Membrane Chromatographic Analysis System for Screening Specific Components That Counteract Doxorubicin-Induced Heart Failure from Acontium carmichaeli

et al. 2014

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Cell membrane chromatography (CMC) derived from pathological tissues is ideal for screening specific components acting on specific diseases from complex medicines owing to the maximum simulation of in vivo drug-receptor interactions. However, there are no pathological tissue-derived CMC models that have ever been developed, as well as no visualized affinity comparison of potential active components between normal and pathological CMC columns. In this study, a novel comparative normal/failing rat myocardium CMC analysis system based on online column selection and comprehensive two-dimensional (2D) chromatography/monolithic column/time-of-flight mass spectrometry was developed for parallel comparison of the chromatographic behaviors on both normal and pathological CMC columns, as well as rapid screening of the specific therapeutic agents that counteract doxorubicin (DOX)-induced heart failure from Acontium carmichaeli (Fuzi). In total, 16 potential active alkaloid components with similar structures in Fuzi were retained on both normal and failing myocardium CMC models. Most of them had obvious decreases of affinities on failing myocardium CMC compared with normal CMC model except for four components, talatizamine (TALA), 14-acetyl-TALA, hetisine, and 14-benzoylneoline. One compound TALA with the highest affinity was isolated for further in vitro pharmacodynamic validation and target identification to validate the screen results. Voltage-dependent K+ channel was confirmed as a binding target of TALA and 14-acetyl-TALA with high affinities. The online high throughput comparative CMC analysis method is suitable for screening specific active components from herbal medicines by increasing the specificity of screened results and can also be applied to other biological chromatography models.

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“…Identified metabolites were found to be associated with pentose and glucuronate interconversions, alanine, aspartate and glutamate metabolism, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, purine metabolism, tryptophan metabolism, taurine and hypotaurine metabolism, fructose and mannose metabolism, and fatty-acid metabolism. Some 14 lipid metabolites involved in phospholipid metabolism, sphingolipid metabolism, saturated fatty acid oxidation and unsaturated fatty acid peroxidation were identified as the potential biomarkers of Fuzi toxicity on heart by Cai et al [93].…”

Section: Investigation and Elucidation Of Tcm Toxicitymentioning

confidence: 99%

Quadrupole time-of-flight mass spectrometry as a powerful tool for demystifying traditional Chinese medicine

Zheng-xiang¹,

Tao²,

Bai

et al. 2015

TrAC Trends in Analytical Chemistry

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Myocardial lipidomics profiling delineate the toxicity of traditional Chinese medicine Aconiti Lateralis radix praeparata

Cited by 61 publications

References 34 publications

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Comparative Normal/Failing Rat Myocardium Cell Membrane Chromatographic Analysis System for Screening Specific Components That Counteract Doxorubicin-Induced Heart Failure from Acontium carmichaeli

Quadrupole time-of-flight mass spectrometry as a powerful tool for demystifying traditional Chinese medicine

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