Myocardial interstitial serotonin and its major metabolite, 5-hydroxyindole acetic acid levels determined by microdialysis technique in rat heart

Du, Cheng-Kun; Zhan, Dong Yun; Akiyama, Tsuyoshi; Sonobe, Takashi; Shirai, Masamitsu

doi:10.1016/j.lfs.2014.09.019

Cited by 6 publications

(11 citation statements)

References 40 publications

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“…The materials and properties of the dialysis probe have been described previously (2,8,33). Briefly, a dialysis fiber of semipermeable membrane (length: 6 mm; outer diameter: 310 m; inner diameter: 200 m; molecular weight cutoff: 50,000; PAN-1200; Asahi Chemical, Tokyo, Japan) was attached at both ends to polyethylene tubes (length: 25 cm; outer diameter: 500 m; inner diameter: 200 m).…”

Section: Cardiac Microdialysis Techniquementioning

confidence: 99%

“…5-HT and 5-HIAA concentrations in the dialysate were measured by HPLC with electrochemical detection (HPLC-ECD; Eicom; Kyoto, Japan). Details of 5-HT and 5-HIAA measurement by HPLC-ECD have been described previously (8).…”

Section: Cardiac Microdialysis Techniquementioning

confidence: 99%

“…A MAO inhibitor, pargyline, was administered locally through the dialysis probe by perfusing Ringer's solution containing pargyline (1 mM). The concentration of pargyline was determined based on our previous studies (8,33,39). The time schedule of ischemia-reperfusion and dialysate sampling was the same as protocol 1.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…In a recent study, we simultaneously monitored myocardial interstitial 5-HT and 5-HIAA levels by the microdialysis technique (8). 5-HIAA is a highly lipophilic metabolite that penetrates the cell membrane by diffusion.…”

mentioning

confidence: 99%

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Myocardial interstitial levels of serotonin and its major metabolite 5-hydroxyindole acetic acid during ischemia-reperfusion

Zhan

Akiyama

et al. 2017

American Journal of Physiology-Heart and Circulatory Physiology

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By monitoring myocardial interstitial levels of 5-HT and its metabolite, 5-hydroxyindole acetic acid, we investigated 5-HT kinetics during myocardial ischemia-reperfusion. 5-HT accumulates but 5-HT degradation is suppressed during ischemia. After reperfusion, 5-HT degradation is enhanced and this degradation is dependent on monoamine oxidase activity but not fluoxetine-sensitive uptake transporter.

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Section: Cardiac Microdialysis Techniquementioning

confidence: 99%

Section: Cardiac Microdialysis Techniquementioning

confidence: 99%

Section: Experimental Protocolsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Myocardial interstitial levels of serotonin and its major metabolite 5-hydroxyindole acetic acid during ischemia-reperfusion

Zhan

Akiyama

et al. 2017

American Journal of Physiology-Heart and Circulatory Physiology

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“…The first dialysate sampling was started from at least 2 hr after the probe implantation. Serotonin concentration in the dialysate sample was measured by electro‐chemical detection coupled with high performance liquid chromatography (HPLC‐ECD, Eicom) as described previously (Du et al, , ; Sonobe et al, ).…”

Section: Methodsmentioning

confidence: 99%

Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo

Sonobe

Akiyama

et al. 2019

Physiol Rep

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Serotonin (5‐HT) accumulates in the heart during myocardial ischemia and induces deleterious effects on the cardiomyocytes through receptor‐dependent and monoamine oxidase‐dependent pathways. We aimed to clarify the involvement of extra‐neuronal monoamine transporters in the clearance of 5‐HT during ischemia and reperfusion in the heart. Using a microdialysis technique in the anesthetized Wistar rat heart, we monitored myocardial interstitial 5‐HT and 5‐hydroxyindole acetic acid (5‐HIAA) concentration by means of electro‐chemical detection coupled with high‐performance liquid chromatography (HPLC‐ECD). Effects of inhibitors of the plasma membrane monoamine transporter (PMAT) and the organic cation transporter 3 (OCT3) (decynium‐22 and corticosterone) on the 5‐HT and 5‐HIAA concentrations during baseline, coronary occlusion, and reperfusion were investigated. Basal dialysate 5‐HT concentration were increased by local administration of decynium‐22, but not by corticosterone. Addition of fluoxetine, a serotonin transporter (SERT) inhibitor further increased the 5‐HT concentration upon during administration of decynium‐22. Decynium‐22 elevated the background level of 5‐HT during coronary occlusion and maintained 5‐HT concentration at a high level during reperfusion. Production of 5‐HIAA in the early reperfusion was significantly suppressed by decynium‐22. These results indicate that PMAT and SERT independently regulate basal level of interstitial 5‐HT, and PMAT plays a more important role in the clearance of 5‐HT during reperfusion. These data suggest the involvement of PMAT in the monoamine oxidase‐dependent deleterious pathway in the heart.

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Simultaneous quantification of neuroactive dopamine serotonin and kynurenine pathway metabolites in gender-specific youth urine by ultra performance liquid chromatography tandem high resolution mass spectrometry

Wang

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Myocardial interstitial serotonin and its major metabolite, 5-hydroxyindole acetic acid levels determined by microdialysis technique in rat heart

Cited by 6 publications

References 40 publications

Myocardial interstitial levels of serotonin and its major metabolite 5-hydroxyindole acetic acid during ischemia-reperfusion

Myocardial interstitial levels of serotonin and its major metabolite 5-hydroxyindole acetic acid during ischemia-reperfusion

Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo

Simultaneous quantification of neuroactive dopamine serotonin and kynurenine pathway metabolites in gender-specific youth urine by ultra performance liquid chromatography tandem high resolution mass spectrometry

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