Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics

Liao, Zihan; Li, Rongxuan; Xia, Wei; Guo, Honghui; Luo, Jiawei; Sheng, Huaxin; Tian, Meihui; Cao, Zhipeng

doi:10.3390/molecules27134284

Cited by 4 publications

(2 citation statements)

References 52 publications

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“…Many previous studies have shown that lipid metabolism is related to many biological functions, being essential for many biochemical reactions ( 32 ). It has been found that the levels of unsaturated fatty acids (e.g., linoleic acid and arachidonic acid) were significantly down-regulated in a high-fat-induced model group, suggesting enhanced peroxidation and oxidative stress ( 33 ). Analysis of liver tissue sections has confirmed that peroxidation and oxidative stress could decompose apolipoprotein B protein, thereby weakening the secretion of very low-density lipoprotein (VLDL), reducing the output of liver TG and facilitating the accumulation of TG in the liver ( 34 ).…”

Section: Resultsmentioning

confidence: 99%

Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics

et al. 2023

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ObjectiveIn this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.MethodsThe structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry. In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group. Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.ResultsThe PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid. The PEPs began to degrade at 251°C (T0), while the maximum thermal degradation rate temperature (Tm) appeared at 300°C. The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities. After PEPs intervention, the metabolic profile of mice changed significantly. A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet. These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).ConclusionsThe presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.

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Section: Resultsmentioning

confidence: 99%

Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics

et al. 2023

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“…Kakimoto et al [86] utilized shotgun proteomics to analyze the mechanism of death in acquired cardiac hypertrophy autopsy, and indicated that the stepwise upregulation of sarcomere protein is a danger signal ACH, which may help to diagnose the sudden cardiac death of ACH. Ma et al [87] selected 56 DEPs with TMT-based LC-MS/MS, and proposed that the chronic ethanol exposure could lead to hypertrophy of cardiomyocytes, and the dilation of the ventricle, etc. Additionally, proteomics also has an obvious advantage in detecting postmortem DEPs for PMI estimation too.…”

Section: Proteomicsmentioning

confidence: 99%

The Role of Protein Degradation in Estimation Postmortem Interval and Confirmation of Cause of Death in Forensic Pathology: A Literature Review

Huang,

Zhao,

Liu

et al. 2024

IJMS

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It is well known that proteins are important bio-macromolecules in human organisms, and numerous proteins are widely used in the clinical practice, whereas their application in forensic science is currently limited. This limitation is mainly attributed to the postmortem degradation of targeted proteins, which can significantly impact final conclusions. In the last decade, numerous methods have been established to detect the protein from a forensic perspective, and some of the postmortem proteins have been applied in forensic practice. To better understand the emerging issues and challenges in postmortem proteins, we have reviewed the current application of protein technologies at postmortem in forensic practice. Meanwhile, we discuss the application of proteins in identifying the cause of death, and postmortem interval (PMI). Finally, we highlight the interpretability and limitations of postmortem protein challenges. We believe that utilizing the multi-omics method can enhance the comprehensiveness of applying proteins in forensic practice.

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Detection of interactions between genetic marker sets and environment in a genome-wide study of hypertension

Shen

Amei

Liu

et al. 2023

Preprint

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SummaryAs human complex diseases are influenced by the interplay of genes and environment, detecting gene-environment interactions (G×E) can shed light on biological mechanisms of diseases and play an important role in disease risk prediction. Development of powerful quantitative tools to incorporateG×Ein complex diseases has potential to facilitate the accurate curation and analysis of large genetic epidemiological studies. However, most of existing methods that interrogateG×Efocus on the interaction effects of an environmental factor and genetic variants, exclusively for common or rare variants. In this study, we proposed two tests, MAGEIT_RAN and MAGEIT_FIX, to detect interaction effects of an environmental factor and a set of genetic markers containing both rare and common variants, based on the MinQue for Summary statistics. The genetic main effects in MAGEIT_RAN and MAGEIT_FIX are modeled as random or fixed, respectively. Through simulation studies, we illustrated that both tests had type I error under control and MAGEIT_RAN was overall the most powerful test. We applied MAGEIT to a genome-wide analysis of gene-alcohol interactions on hypertension in the Multi-Ethnic Study of Atherosclerosis. We detected two genes,CCNDBP1andEPB42, that interact with alcohol usage to influence blood pressure. Pathway analysis identified sixteen significant pathways related to signal transduction and development that were associated with hypertension, and several of them were reported to have an interactive effect with alcohol intake. Our results demonstrated that MAGEIT can detect biologically relevant genes that interact with environmental factors to influence complex traits.

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Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics

Cited by 4 publications

References 52 publications

Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics

Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics

The Role of Protein Degradation in Estimation Postmortem Interval and Confirmation of Cause of Death in Forensic Pathology: A Literature Review

Detection of interactions between genetic marker sets and environment in a genome-wide study of hypertension

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