Myocardial Infarction by Percutaneous Embolization Coil Deployment in a Swine Model

Martínez-Falguera, Daina; Fadeuilhe, Edgar; Teis, Albert; Aranyo, J; Adeliño, Raquel; Bisbal, Felipe; Rodríguez-Leor, Oriol; Gálvez‐Montón, Carolina

doi:10.3791/63172-v

“…Animals underwent a nonreperfused anterior MI procedure as previously described. 20 MI was induced by percutaneous selective coil embolization (VortX-18 Diamond 3-mm/3.3-mm coil; Boston Scientific/Target, Natick, MA) of the left anterior descending coronary artery after the first diagonal branch, under general anesthesia. Complete thrombotic occlusion was confirmed by persistent ST-segment elevation on the ECG and by repeating the coronary angiography 10 minutes after coil implantation (Thrombolysis in Myocardial Infarction flow score of 0).…”

Section: Methodsmentioning

confidence: 99%

Antiarrhythmic and Anti-Inflammatory Effects of Sacubitril/Valsartan on Post-Myocardial Infarction Scar

Martínez-Falguera,

Aranyó,

Teis

et al. 2024

Circ: Arrhythmia and Electrophysiology

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BACKGROUND: Sacubitril/valsartan (Sac/Val) is superior to angiotensin-converting enzyme inhibitors in reducing the risk of heart failure hospitalization and cardiovascular death, but its mechanistic data on myocardial scar after myocardial infarction (MI) are lacking. The objective of this work was to assess the effects of Sac/Val on inflammation, fibrosis, electrophysiological properties, and ventricular tachycardia inducibility in post-MI scar remodeling in swine. METHODS: After MI, 22 pigs were randomized to receive β-blocker (BB; control, n=8) or BB+Sac/Val (Sac/Val, n=9). The systemic immune response was monitored. Cardiac magnetic resonance data were acquired at 2-day and 29-day post MI to assess ventricular remodeling. Programmed electrical stimulation and high-density mapping were performed at 30-day post MI to assess ventricular tachycardia inducibility. Myocardial samples were collected for histological analysis. RESULTS: Compared with BB, BB+Sac/Val reduced acute circulating leukocytes ( P =0.009) and interleukin-12 levels ( P =0.024) at 2-day post MI, decreased C-C chemokine receptor type 2 expression in monocytes ( P =0.047) at 15-day post MI, and reduced scar mass ( P =0.046) and border zone mass ( P =0.043). It also lowered the number and mass of border zone corridors ( P =0.020 and P =0.05, respectively), scar collagen I content ( P =0.049), and collagen I/III ratio ( P =0.040). Sac/Val reduced ventricular tachycardia inducibility ( P =0.026) and the number of deceleration zones ( P =0.016). CONCLUSIONS: After MI, compared with BB, BB+Sac/Val was associated with reduced acute systemic inflammatory markers, reduced total scar and border zone mass on late gadolinium-enhanced magnetic resonance imaging, and lower ventricular tachycardia inducibility.

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“…describe an MI model obtained by permanent coil deployment in swine that may be beneficial in exploring new therapeutic options 6 . The described method is proven to be feasible and highly reproducible.…”

mentioning

confidence: 99%

“…It avoids the need for open-heart procedures and the accompanying inflammatory reaction that takes place after surgery. An important advantage is that this model reproduces the pathogenesis of non-revascularized MI found in humans and it is appropriate for MI models in their acute, sub-acute and even chronic phase, depending on the follow-up duration of a given study 6 .…”

mentioning

confidence: 99%

Large Animal Models of Cardiovascular Disease: From Training to Translation

Báez-Díaz

¹

,

Crisóstomo

²

2023

JoVE

0

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Constantin, I., Tăbăran, A. F. Dissection techniques and histological sampling of the heart in large animal models for cardiovascular diseases. Journal of Visualized Experiments. (184), e63809 (2022). Hao, Y. et al. Transcatheter pulmonary valve replacement from autologous pericardium with a self-expandable nitinol stent in an adult sheep model. Journal of Visualized Experiments. (184), e63661 (2022). Martínez-Falguera, D. et al. Myocardial infarction by percutaneous embolization coil deployment in a swine model. Journal of Visualized Experiments. (177), e63172 (2021). Tubeeckx, M. R. L. et al. Sterile pericarditis in aachener minipigs as a model for atrial myopathy and atrial fibrillation. Journal of Visualized Experiments. (175), e63094 (2021). Discussion Cardiovascular diseases (CVDs) represent the main cause of morbidity and mortality worldwide 1 . Consequently, research in this field is required. While somewhat controversial, especially if not done right, the use of animals for research and training purposes remains a mandatory step of translational research. Small animal models have contributed considerably to unraveling the cellular and molecular mechanisms of CVDs, but extrapolation to a clinical scenario is hindered by the remarkable cardiovascular dissimilarities with humans 2 , 3 . Hence there is importance in large animal models, as they are closer to humans with respect to size as well as anatomical and physiological features 3 , 4 .

show abstract

Large Animal Models of Cardiovascular Disease: From Training to Translation

Báez-Díaz

¹

,

Crisóstomo

²

2023

JoVE

1

0

View full text Add to dashboard Cite

Constantin, I., Tăbăran, A. F. Dissection techniques and histological sampling of the heart in large animal models for cardiovascular diseases. Journal of Visualized Experiments. (184), e63809 (2022). Hao, Y. et al. Transcatheter pulmonary valve replacement from autologous pericardium with a self-expandable nitinol stent in an adult sheep model. Journal of Visualized Experiments. (184), e63661 (2022). Martínez-Falguera, D. et al. Myocardial infarction by percutaneous embolization coil deployment in a swine model. Journal of Visualized Experiments. (177), e63172 (2021). Tubeeckx, M. R. L. et al. Sterile pericarditis in aachener minipigs as a model for atrial myopathy and atrial fibrillation. Journal of Visualized Experiments. (175), e63094 (2021). Discussion Cardiovascular diseases (CVDs) represent the main cause of morbidity and mortality worldwide 1 . Consequently, research in this field is required. While somewhat controversial, especially if not done right, the use of animals for research and training purposes remains a mandatory step of translational research. Small animal models have contributed considerably to unraveling the cellular and molecular mechanisms of CVDs, but extrapolation to a clinical scenario is hindered by the remarkable cardiovascular dissimilarities with humans 2 , 3 . Hence there is importance in large animal models, as they are closer to humans with respect to size as well as anatomical and physiological features 3 , 4 .

show abstract

Myocardial Infarction by Percutaneous Embolization Coil Deployment in a Swine Model

Cited by 4 publications

References 0 publications

Antiarrhythmic and Anti-Inflammatory Effects of Sacubitril/Valsartan on Post-Myocardial Infarction Scar

Antiarrhythmic and Anti-Inflammatory Effects of Sacubitril/Valsartan on Post-Myocardial Infarction Scar

Large Animal Models of Cardiovascular Disease: From Training to Translation

Large Animal Models of Cardiovascular Disease: From Training to Translation

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