Myocardial Infarction–Associated Transcript, a Long Noncoding RNA, Is Overexpressed During Dilated Cardiomyopathy Due to Chronic Chagas Disease

Abstract

Long noncoding RNAs (lncRNAs) modulate gene expression at the epigenetic, transcriptional, and posttranscriptional levels. Dysregulation of the lncRNA known as myocardial infarction-associated transcript (MIAT) has been associated with myocardial infarction. Chagas disease causes a severe inflammatory dilated chronic cardiomyopathy (CCC). We investigated the role of MIAT in CCC. A whole-transcriptome analysis of heart biopsy specimens and formalin-fixed, paraffin-embedded samples revealed that MIAT was overexpressed in patients with CCC, compared with subjects with noninflammatory dilated cardiomyopathy and controls. These results were confirmed in a mouse model. Results suggest that MIAT is a specific biomarker of CCC.

WITHDRAWN: Downregulation of AC061961.2, LINGO1-AS1, and RP11-13E1.5 is associated with dilated cardiomyopathy progression

“…They identified 14 lncRNAs deregulated in these patients, such as CDKN2B-AS1/ANRIL, EGOT and H19. Additionally, Frade et al [16] performed a whole-transcriptome analysis of heart biopsy specimens of DCM patients and healthy hearts donors and revealed that MIAT was overexpressed in patients with DCM. Whereas, these lncRNAs were not identified in our study, which may be due to the sample heterogeneity.…”

“…Several recent studies have identified many lncRNAs associated with diseased hearts [8][9][10]. However, there are still not enough studies on the functions of lncRNAs in DCM [11,12].…”

Whole-Genome Cardiac DNA Methylation Fingerprint and Gene Expression Analysis Provide New Insights in the Pathogenesis of Chronic Chagas Disease Cardiomyopathy

“…Human left ventricular free wall heart tissue samples were obtained from patients with end-stage heart failure CCC at the time of heart transplantation (n = 25). CCC patients underwent serological diagnosis of T. cruzi infection and standard electrocardiography and echocardiography, and tissues were subject to histopathological assessment [ 13 ]. Biopsies from controls (n = 7) were obtained from healthy hearts of organ donors having no suitable recipient ( Table 1 ).…”

Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury

“…Recently, lncRNAs are recognized as additional players in cardiomyopathies (Table 3). 131,132 A recent work tested whether circulating mitochondrial lncRNAs might serve as biomarkers of cardiac remodeling in HCM patients. Two transcripts, uc004cov.4 and uc022bqu.1, were elevated in the circulation of hypertrophic obstructive cardiomyopathy patients when compared with hypertrophic nonobstructive cardiomyopathy and healthy subjects.…”