Myocardial gene expression alterations in peripheral blood mononuclear cells of patients with idiopathic dilated cardiomyopathy

Kontaraki, Joanna; Parthenakis, Fragiskos I.; Nyktari, Eva; Patrianakos, Alexandros; Vardas, Panos

doi:10.1093/eurjhf/hfq057

Cited by 21 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Levels of MYOCD mRNA were first shown to be elevated in humans with DCM as compared to normal hearts[81]. Moreover, there have been a series of clinical studies showing increases in MYOCD mRNA in circulating cells or cardiac tissue from patients with idiopathic cardiomyopathy[121],[122], hypertrophic cardiomyopathy[123], and essential hypertension[124]. Interestingly, variants in the 5′ promoter region of human MYOCD were associated with reduced levels of circulating MYOCD , which correlated with attenuated left ventricular mass in hypertrophic cardiomyopathy patients[125].…”

Section: Pathological Processes Linked To Myocardinmentioning

confidence: 99%

Myocardin in biology and disease

Miano

2015

J Biomed Res

View full text Add to dashboard Cite

Myocardin (MYOCD) is a potent transcriptional coactivator that functions primarily in cardiac muscle and smooth muscle through direct contacts with serum response factor (SRF) over cis elements known as CArG boxes found near a number of genes encoding for contractile, ion channel, cytoskeletal, and calcium handling proteins. Since its discovery more than 10 years ago, new insights have been obtained regarding the diverse isoforms of MYOCD expressed in cells as well as the regulation of MYOCD expression and activity through transcriptional, post-transcriptional, and post-translational processes. Curiously, there are a number of functions associated with MYOCD that appear to be independent of contractile gene expression and the CArG-SRF nucleoprotein complex. Further, perturbations in MYOCD gene expression are associated with an increasing number of diseases including heart failure, cancer, acute vessel disease, and diabetes. This review summarizes the various biological and pathological processes associated with MYOCD and offers perspectives to several challenges and future directions for further study of this formidable transcriptional coactivator.

show abstract

Section: Pathological Processes Linked To Myocardinmentioning

confidence: 99%

Myocardin in biology and disease

Miano

2015

J Biomed Res

View full text Add to dashboard Cite

show abstract

“…In adult heart, SERCA2 expression levels are highly associated with cardiac functions, therefore, SERCA2 could be used as a marker for detection of cardiomyopathy (Castilho et al, 2007;Andersson et al, 2009;Dai and Rabinovitch, 2009;Kontaraki et al, 2010;Stokke et al, 2010;Summerfield et al, 2010). We have shown that atp2a2a transcripts were detected strongly in the developing heart of 18-96 hpf zebrafish embryos (Fig.…”

Section: Atp2a2a Might Play Important Roles During Heart Development mentioning

confidence: 77%

Molecular structure and developmental expression of zebrafish atp2a genes

Lai

Pai²,

Tsai

et al. 2011

Genes Genom

View full text Add to dashboard Cite

We isolated two atp2a genes, atp2a1 and atp2a2a, from embryonic zebrafish. Amino acid sequences deduced from zebrafish atp2a genes are aligned with orthologue proteins from other species, the results showed that they share high percentage of identities (82%-94%) and acidic pIs (5.03-5.33). Whole mount in situ hybridization experiments showed that atp2a1 and atp2a2a are maternal inherited genes which can be detected at 1-cell stage embryos and express in the entire animal pole from 6 hours post-fertilization (hpf) to 12 hpf. At the later stages (48-96 hpf), expression of atp2a1 was restricted in head and trunk muscles as well as in some neurons. In contrast to the strongly expression of atp2a1 in head muscle, expression of atp2a2a was detected in head muscle in a fainter manner. In addition, transcripts of atp2a2a were observed in the developing heart during early cardiogenesis. The present studies not only help us to comparatively analyze atp2a genes across species, but also provide useful information about expressions during early embryogenesis that will help in further investigations of functional studies of Atp2a in the future.

show abstract

“…Sarcoplasmic Endoplasmic Reticulum Ca 2+ ATPase (SERCA) is considered a major player in these processes [10]. Indeed, patients with AF have lower SERCA levels compared to patients with sinus rhythm [13]. Of note, SERCA levels can be assayed in peripheral blood lymphocytes, as their levels correlate with SERCA levels obtained in specimens of cardiac tissue [13].…”

Section: Introductionmentioning

confidence: 99%

Modulation of SERCA in Patients with Persistent Atrial Fibrillation Treated by Epicardial Thoracoscopic Ablation: The CAMAF Study

Sardu

Santulli

Guerra

et al. 2020

JCM

View full text Add to dashboard Cite

Objectives: To evaluate atrial fibrillation (AF) recurrence and Sarcoplasmic Endoplasmic Reticulum Calcium ATPase (SERCA) levels in patients treated by epicardial thoracoscopic ablation for persistent AF. Background: Reduced levels of SERCA have been reported in the peripheral blood cells of patients with AF. We hypothesize that SERCA levels can predict the response to epicardial ablation. Methods: We designed a prospective, multicenter, observational study to recruit, from October 2014 to June 2016, patients with persistent AF receiving an epicardial thoracoscopic pulmonary vein isolation. Results: We enrolled 27 patients. Responders (n = 15) did not present AF recurrence after epicardial ablation at one-year follow-up; these patients displayed a marked remodeling of the left atrium, with a significant reduction of inflammatory cytokines, B type natriuretic peptide (BNP), and increased levels of SERCA compared to baseline and to nonresponders (p < 0.05). Furthermore, mean AF duration (Heart rate (HR) 1.235 (1.037–1.471), p < 0.05), Left atrium volume (LAV) (HR 1.755 (1.126–2.738), p < 0.05), BNP (HR 1.945 (1.895–1.999), p < 0.05), and SERCA (HR 1.763 (1.167–2.663), p < 0.05) were predictive of AF recurrence. Conclusions: Our data indicate for the first time that baseline values of SERCA in patients with persistent AF might be predictive of failure to epicardial ablative approach. Intriguingly, epicardial ablation was associated with increased levels of SERCA in responders. Therefore, SERCA might be an innovative therapeutic target to improve the response to epicardial ablative treatments.

show abstract

Myocardial gene expression alterations in peripheral blood mononuclear cells of patients with idiopathic dilated cardiomyopathy

Cited by 21 publications

References 31 publications

Myocardin in biology and disease

Myocardin in biology and disease

Molecular structure and developmental expression of zebrafish atp2a genes

Modulation of SERCA in Patients with Persistent Atrial Fibrillation Treated by Epicardial Thoracoscopic Ablation: The CAMAF Study

Contact Info

Product

Resources

About