Background: Cardiac MRI plays a critical role in the management of thalassemic patients. No accurate biventricular reference values are available. Purpose: To establish the ranges for normal left ventricular (LV) and right ventricular (RV) volumes and ejection fraction (EF) and LV mass normalized to body surface area (BSA), age, and gender in a large cohort of well-treated betathalassemia major (β-TM) patients without heart damage using a multiparametric MRI. Study Type: Retrospective/cohort study. Population: In all, 251 β-TM patients with no known risk factors or cardiac disease, normal electrocardiogram, no macroscopic myocardial fibrosis, and all cardiac segments with T 2 * ≥20 msec, and 246 healthy subjects. Field Strength/Sequence: 1.5T/cine steady-state free precession (SSFP), gradient-echo T 2 *, late gadolinium enhancement (LGE) images. Assessment: Biventricular end-diastolic volume, end-systolic volume, stroke volume, and LV mass were normalized to BSA (EDVI, ESVI, SVI). Statistical Tests: Comparisons between the two groups was performed with two-samples t-test or Wilcoxon's signed rank test. For more than two groups, one-way analysis of variance (ANOVA) or a Kruskal-Wallis test were applied. Results: Compared to controls, males with β-TM showed significantlt higher LVEDVI in all the age groups, while for the other volumes the difference was significant only within one or more age groups. In females the volumes were comparable between β-TM patients and healthy subjects in all the age groups. In the male β-TM population we found a significant effect of age on LVEDVI