Myocardial cell shape change as a mechanism of embryonic heart looping

Manasek, Francis J.; Burnside, M.Beth; Waterman, Robert E.

doi:10.1016/0012-1606(72)90077-2

Cited by 118 publications

(107 citation statements)

References 13 publications

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“…This process likely was slow due to viscous forces. These results suggest that surface tension was a main cause of heart rotation in previous labeling experiments with the splanchnopleura removed (Stalsberg and DeHaan, 1969;Castro-Quezada et al, 1972;Manasek et al, 1972;De la Cruz et al, 1977).…”

Section: Discussionmentioning

confidence: 51%

“…In later experiments with isolated hearts, Butler (1952) and Manning and McLachlan (1990) found that the bending is likely intrinsic to the heart tube. Moreover, as heart rotation has been observed in labeling experiments after the splanchnopleura is removed (Stalsberg and DeHaan, 1969;Castro-Quezada et al, 1972;Manasek et al, 1972;De la Cruz et al, 1977), Manasek et al (1984) postulated that this process also is intrinsic to the heart. However, in all these experiments in which the splanchnopleura was removed, the influence of surface tension on the process of C-looping was not excluded.…”

Section: Discussionmentioning

confidence: 97%

“…To visualize heart rotation, we placed small charcoal particles along the ventral midline of the heart (see Fig. 6) (Stalsberg and DeHaan, 1969;Castro-Quezada et al, 1972;Manasek et al, 1972;De la Cruz et al, 1977); (2) Longitudinal dissection of the right splanchnopleura along a line parallel to the embryo at HH stage 8 -9, as Lepori (1967) described (Fig. 7).…”

Section: Microsurgical Operationsmentioning

confidence: 99%

“…By using this method, we have shown that surface tension can interfere with C-looping in some experiments for which traditional cultivation methods are used (Lepori, 1967;Stalsberg and DeHaan, 1969;Castro-Quezada et al, 1972;Manasek et al, 1972;De la Cruz et al, 1977). In some cases, surface tension can affect results dramatically.…”

Section: Introductionmentioning

confidence: 99%

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Cardiac looping in experimental conditions: Effects of extraembryonic forces

Voronov

Taber

2002

Developmental Dynamics

105

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The chick embryo is a popular experimental model used to study the mechanisms of cardiac looping. To facilitate oxygen transport, researchers typically culture the embryo on the surface of the medium. Such preparations, however, expose the embryo and the heart to surface tension that is not present in ovo. This study investigates the influence that surface and extraembryonic membrane tensions have on looping morphology. To eliminate surface tension, we developed a technique in which the embryo is cultured under a thin layer of fluid. To eliminate membrane tension, the membrane was removed. Our results show that both tensions can affect looping, with surface tension potentially having a much greater effect. Moreover, we show that surface tension can alter results in one classic looping experiment.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 97%

Section: Microsurgical Operationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cardiac looping in experimental conditions: Effects of extraembryonic forces

Voronov

Taber

2002

Developmental Dynamics

105

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“…Actin is a major component of looping (Manasek et al, 1972;Manasek, 1976b;Itasaki et al, 1991;Latacha et al, 2005). Development of actin filaments considerably differ in inner and outer myocardial cell layers (Shiraishi et al, 1992).…”

Section: Actomyosin Cytoskeleton and Heart Loopingmentioning

confidence: 99%

Cellular nonmuscle myosins NMHC‐IIA and NMHC‐IIB and vertebrate heart looping

Lu¹,

Seeholzer²,

Han³

et al. 2008

Developmental Dynamics

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Flectin, a protein previously described to be expressed in a left-dominant manner in the embryonic chick heart during looping, is a member of the nonmuscle myosin II (NMHC-II) protein class. During looping, both NMHC-IIA and NMHC-IIB are expressed in the mouse heart on embryonic day 9.5. The patterns of localization of NMHC-IIB, rather than NMHC-IIA in the mouse looping heart and in neural crest cells, are equivalent to what we reported previously for flectin. Expression of full-length human NMHC-IIA and -IIB in 10 T1/2 cells demonstrated that flectin antibody recognizes both isoforms. Electron microscopy revealed that flectin antibody localizes in short cardiomyocyte cell processes extending from the basal layer of the cardiomyocytes into the cardiac jelly. Flectin antibody also recognizes stress fibrils in the cardiac jelly in the mouse and chick heart; while NMHC-IIB antibody does not. Abnormally looping hearts of the Nodal ⌬ 600 homozygous mouse embryos show decreased NMHC-IIB expression on both the mRNA and protein levels. These results document the characterization of flectin and extend the importance of NMHC-II and the cytoskeletal actomyosin complex to the mammalian heart and cardiac looping. Developmental Dynamics 237:3577-3590, 2008.

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