Myoblast-Acellular Skeletal Muscle Matrix Constructs Guarantee a Long-Term Repair of Experimental Full-Thickness Abdominal Wall Defects

Coppi, Paolo De; Bellini, Silvia; Conconi, Maria Teresa; Sabatti, M.; Simonato, Enea; Gamba, Pier Giorgio; Nussdorfer, Gastone G.; Parnigotto, Pier Paolo

doi:10.1089/ten.2006.12.ft-82

Cited by 16 publications

(26 citation statements)

References 19 publications

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“…Moreover, decellularized muscle seeded with autologous myogenic cells can be used to functionally repair abdominal defects (22)(23), and growth factors may further promote vascularization and, therefore, longterm functional results (21,24). This study significantly adds to our previous data by demonstrating how the implanted scaffolds can modulate the immune response in several ways, thereby inhibiting rejection in a xenotransplantation model and preventing the rejection of donor-derived xenogeneic cells for up to 4 wk in vivo.…”

Section: Discussionmentioning

confidence: 68%

“…They are CCR7 + CD86 + and are inducer and effector cells in TH1-type inflammatory and rejection responses as observed when the fresh tissue was implanted. M2-activated macrophages, however, express an IL-12 low , IL-23 low , and IL-10 high phenotype and are able to facilitate tissue repair, constructive remodelling through ECM construction, and angiogenesis, which could partially be responsible for the regeneration observed when decellularized muscles are implanted (22,23,27). M2 macrophages are CD163 + Arg I + and predominantly induce a classical TH2 response that is anti-inflammatory and is hypothesized to be particularly beneficial for constructive tissue remodelling and skeletal muscle regeneration (41,42).…”

Section: Discussionmentioning

confidence: 99%

“…The field continues to expand and tissue bioengineering has provided, or is close to delivering, functional human organ replacements elsewhere (6,7,(13)(14)(15)(16)(17). The ability to produce innervated and revascularized muscles would hugely extend the possible applications of regenerative medicine (18)(19)(20)(21)(22)(23)(24)(25)(26).…”

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confidence: 99%

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Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Fishman

Lowdell

Urbani³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

178

133

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Decellularized (acellular) scaffolds, composed of natural extracellular matrix, form the basis of an emerging generation of tissueengineered organ and tissue replacements capable of transforming healthcare. Prime requirements for allogeneic, or xenogeneic, decellularized scaffolds are biocompatibility and absence of rejection. The humoral immune response to decellularized scaffolds has been well documented, but there is a lack of data on the cellmediated immune response toward them in vitro and in vivo. Skeletal muscle scaffolds were decellularized, characterized in vitro, and xenotransplanted. The cellular immune response toward scaffolds was evaluated by immunohistochemistry and quantified stereologically. T-cell proliferation and cytokines, as assessed by flow cytometry using carboxy-fluorescein diacetate succinimidyl ester dye and cytometric bead array, formed an in vitro surrogate marker and correlate of the in vivo host immune response toward the scaffold. Decellularized scaffolds were free of major histocompatibility complex class I and II antigens and were found to exert anti-inflammatory and immunosuppressive effects, as evidenced by delayed biodegradation time in vivo; reduced sensitized T-cell proliferative activity in vitro; reduced IL-2, IFN-γ, and raised IL-10 levels in cell-culture supernatants; polarization of the macrophage response in vivo toward an M2 phenotype; and improved survival of donor-derived xenogeneic cells at 2 and 4 wk in vivo. Decellularized scaffolds polarize host responses away from a classical TH1-proinflammatory profile and appear to down-regulate T-cell xeno responses and TH1 effector function by inducing a state of peripheral T-cell hyporesponsiveness. These results have substantial implications for the future clinical application of tissue-engineered therapies.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Fishman

Lowdell

Urbani³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

178

133

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“…Reconstruction of the abdominal wall is important for extensive resections from surgery or large tissues that are lost during traumatic injuries 1 . Currently, there are many biological scaffold materials from human cadavers or other animal sources utilized for surgical treatment of abdominal wall defects, such as human acellular dermal matrix (HADM), small intestine submucosa (SIS) and so on.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

et al. 2014

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. Design of the study, critical revision. ABSTRACT PURPOSE:To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects. METHODS:Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPRO TM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization. RESULTS:After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation. CONCLUSION:Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.Key words: Abdominal Wall. Biocompatible Materials. Intestinal, Fibroblast Growth Factor 2. Rats. Reconstruction of abdominal wall defects using small intestinal submucosa coated withgelatin hydrogel incorporating basic fibroblast growth factor

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“…Decellularized muscle seeded with myoblasts has been shown to be capable of generating a contractile force on electrical stimulation [17]. However decellularization of muscle has so far been limited to very small muscles or fragments of muscle and in vivo successes are limited to small animal models [18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Decellularization of Human Lip

Matthew¹,

Butler²

2015

J Tissue Sci Eng

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Myoblast-Acellular Skeletal Muscle Matrix Constructs Guarantee a Long-Term Repair of Experimental Full-Thickness Abdominal Wall Defects

Cited by 16 publications

References 19 publications

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

Decellularization of Human Lip

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