Myiasis caused by Cephalopina titillator (Diptera: Oestridae) in camels (Camelus dromedarius) of semi-arid areas in Iran: distribution and associated risk factors

Jalali, Mohammad Hossein Razi; Dehghan, Saeed; Haji, Abdollah; Ebrahimi, Mohsen

doi:10.1007/s00580-016-2246-9

Cited by 9 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Essential oils of Egyptian Z. officinale and C. rotundus are a well-known functional food and traditional herbal medicine [36,37] .Some researchers demonstrated that the whole essential oils are more efficient than individual constituents or even a combination of constituents [38], they effectively destroy several bacterial, fungal, and viral pathogens. The efficacy of veterinary products or medicines tested by researchers indicated that the overall efficacy of ectoparasiticides for the treatment of infestations by diptera species should be between 80 and 100 % [3,5,39]. The efficacy of C. rotundus and Z. officinale oils met these criteria in laboratory larval bioassays.…”

Section: Discussionmentioning

confidence: 99%

“…During the fly life-cycle, the female enters the camel's nostrils and deposits its larvae into the nasal cavity.Few reports of the infection of camels with C. titillatorin the world are found. A high incidence has been reported in Chad and other parts of West Africa as well as Sudan and Egypt [2][3][4][5] .Cephalopina infestations impair an animal's physiological functions, damage tissues and cause economic losses through abortion, loss of weight gain, reduction of camel fertility and hide quality [6,7] . Jalali et al [5]demonstrated that infested camels show difficulty in breathing, restlessness and loss of appetite which may lead to a cessation of feeding.…”

Section: Introductionmentioning

confidence: 99%

“…A high incidence has been reported in Chad and other parts of West Africa as well as Sudan and Egypt [2][3][4][5] .Cephalopina infestations impair an animal's physiological functions, damage tissues and cause economic losses through abortion, loss of weight gain, reduction of camel fertility and hide quality [6,7] . Jalali et al [5]demonstrated that infested camels show difficulty in breathing, restlessness and loss of appetite which may lead to a cessation of feeding. The severity of clinical signs depends on the amount of damage caused by migrating larvae [7].Camels exhibited congestion, swollen and edematous mucous membranes of the nasopharynx and frontal sinuses [2,8].…”

Section: Introductionmentioning

confidence: 99%

“…For example, Z. officinale (Roscoe), commonly called ginger, is used for a variety of purposes, including culinary and medicinal. In ancient times, ginger has been used in traditional medicine for respiratory troubles, stroke, inflammation, hyper-cholesterolaemia, helminthiasis, and larvicidal effect [23,5] .This plant still attracts extensive research attention.…”

Section: Introductionmentioning

confidence: 99%

“…Researchers have reported that the deleterious effects of Schistosoma mansoni could be minimize after oral administration of ginger extract in experimentally infected mice [5]. Cyperusrotundus L. (Cyperaceae) is a weed that grows in fields and on the canal borders.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Larvicidal Effect of Zingiber officinale and Cyperusrotundus Oils on The third Instar Larvae Cephalopinatitillator (L.)

Moghazy

Namaky

2019

NIDOC-ASRT

View full text Add to dashboard Cite

C ephalopina titillator (C. titillator) [Clark, Diptera: Oestridae] only attacks camels and damage tissues causing economic losses and reduction of fertility. The present work evaluated two common essential plant oils from Cyperusrotundus (C. rotundus) (Cyperaceae) and Zingiber officinale (Z .officinale) (Zingiberaceae), which are considered ecofriendly and have the ability to kill larvae. Scanning electron microscopic examinations were done to demonstrate alterations of the respiratory spiracles after exposure to the essential oils. Three different concentrations 30, 50, 100 µl/ml of oils in distilled water were prepared. Few drops of Tween 80 were added as an emulsifier to the used essential oils. Five 3 rd instar larvae were used in each treatment then dipping procedure was applied five times. Each group of larvae was immersed for 60s in each dilution for each oil and the solution was continuously stirred. Dead larvae were counted after 24 hrs. Samples of treated and control larvae were fixed for Scanning Electron Microscope (SEM). The essential oils were very toxic to C. titillator larvae. Results of dipping bioassays showed that mortality of treated larvae was concentration-dependent. At the 100µl/ml concentration, there was 100% mortality of larvaefor both oils. The posterior spiracles were damaged and showed severe distortion of both lips. C. rotundus and Z. officinale oils have great potential to be developed as a novel larvicidea gainst C. titillator larvae and they could be used as nasal drench .They may be an effective, inexpensive control for this pest.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Larvicidal Effect of Zingiber officinale and Cyperusrotundus Oils on The third Instar Larvae Cephalopinatitillator (L.)

Moghazy

Namaky

2019

NIDOC-ASRT

View full text Add to dashboard Cite

show abstract

Serodiagnosis of nasal myasis in camels (Camelus dromedaries) in Egypt using third larval instar affinity-purified glycoprotein

Aboelsoued,

Toaleb,

Mohamed

et al. 2024

Vet Res Commun

View full text Add to dashboard Cite

The larvae of Cephalopina titillator cause nasopharyngeal myiasis in camels, which parasitize the living tissues of the nasal and paranasal sinuses, pharynx, and larynx. C. titillator infestation adversely affects camel health, meat, and milk production, and can even cause death. In our study, to improve the immunodiagnosis of camel nasal myiasis, a sensitive and specific enzyme-linked immunosorbent assay (ELISA) was developed and evaluated using the Concanavalin-A (Con-A) affinity purification for the C. titillator-N-acetylglucosamine (Ct-GlucNAc) glycoprotein fraction from third larval instars as an antigen for detecting C. titillator antibodies. Crude antigens were prepared from larval instars of C. titillator and evaluated by indirect ELISA. The third C. titillator larval antigen (L3Ct) had the highest protein content (P < 0.001) and the best diagnostic value; chi-square = 235 (P < 0.001). Four glycoprotein fractions were purified separately from the L3Ct antigen by Con-A purification and evaluated. The Ct-GlucNAc glycoprotein fraction was the fraction of choice with the highest diagnostic accuracy (P < 0.05). Using Ct-GlucNAc as a coating antigen, indirect ELISA showed a 99.3% sensitivity for positive results in camel myiasis samples and 100% specificity for negative results in healthy camel samples. The diagnostic accuracy was 99.7%, and no cross reactivity was detected for other parasitic diseases. The indirect ELISA results were confirmed by the western immunoblotting which was characterized by comparing sera from naturally infested dromedary camels with C. titillator, sera from healthy camels and sera from camels with other parasitic infections (Echinococcus granulosus, Fasciola gigantica, Hard ticks; Hyalomma dromedarii, Trichostronglid sp., Eimeria spp., and Cryptosporidium sp.). Immunoreactive antigenic bands of 63, 50, 30 and 18 kDa were predominantly detected in sera from camels with nasopharyngeal myiasis and didn’t react with healthy and camel’s sera from other parasitic infections. However, seven immunoreactive bands appeared at 120, 70, 63, 48, 35, 29, and 19 kDa in the crude L3Ct antigen. In addition, a positive rate of C. titillator immunodiagnosis was detected by indirect ELISA (48.6%, chi-square = 483, P < 0.001), which was significantly greater than that of postmortem diagnosis (31%). In conclusion, the current study introduces a new diagnostic immunoaffinity glycoprotein fraction of C. titillator 3rd larval instar-based ELISA as a highly accurate, simple and fast method to detect specific antibodies of nasal myiasis in camels.

show abstract

Advanced immunological studies on Cephalopina titillator with special references to the epidemiological uses of Dot-ELISA in camel sera

et al. 2020

View full text Add to dashboard Cite

Myiasis caused by Cephalopina titillator (Diptera: Oestridae) in camels (Camelus dromedarius) of semi-arid areas in Iran: distribution and associated risk factors

Cited by 9 publications

References 11 publications

Larvicidal Effect of Zingiber officinale and Cyperusrotundus Oils on The third Instar Larvae Cephalopinatitillator (L.)

Larvicidal Effect of Zingiber officinale and Cyperusrotundus Oils on The third Instar Larvae Cephalopinatitillator (L.)

Serodiagnosis of nasal myasis in camels (Camelus dromedaries) in Egypt using third larval instar affinity-purified glycoprotein

Advanced immunological studies on Cephalopina titillator with special references to the epidemiological uses of Dot-ELISA in camel sera

Contact Info

Product

Resources

About