Myeloprotective activity of crude methanolic leaf extract of Cassia occidentalis in cyclophosphamide-induced bone marrow suppression in Wistar rats

Abstract

Background:Myelosuppression is the most common dose-limiting side effect of chemotherapy. Cassia occidentalis plays a vital role in preventing health disorders, but its hematological effects have not been documented much. This study is designed to investigate the myeloprotective activity of the crude methanolic leaf extract of C. occidentalis in cyclophosphamide-induced bone marrow suppression.Materials and Methods:Twenty-eight Wistar rats aged two to three months, weighing 120-170 g were used for the study. The rats were divided into four groups of seven rats each, labeled A to D. Groups A and B were administered with 3 mg/kg of cyclophosphamide intraperitoneally daily for three days to induce bone marrow suppression, after which groups B and C were orally fed with 250 mg/kg body weight of the crude leaf extract once daily for 14 days. Group D served as control without receiving the extract. On Day 15, blood samples (3.0 ml) were collected from each rat through the retro-orbital plexus of the median canthus into K3-EDTA containers for hematological analysis using standard operative procedures. Data were analyzed with Pearson's correlation test and multivariate analysis of variance using Statistical Package for Social Sciences (SPSS) version 17 and results were expressed as mean ± SD. The level of significance was determined at 95% confidence level.Results:Myelosuppression was achieved in Group A rats. Group B rats showed a significant increase in hemoglobin (Hb), hematocrit (Hct), and total white blood cell count (TWBC) compared with Group A. The Group C rats revealed a significant increase (P < 0.05) in Hb, Hct and TWBC when compared with control.Conclusions:Crude methanolic leaf extract of C. occidentalis may possess myeloprotective properties when orally administered in cyclophosphamide-induced bone marrow suppression.

Shuanghuang Shengbai granule cures myelosuppression and suppresses lung cancer progression: mechanism and therapeutic targets from the aspect of microRNAs

“…In the present study, CTX remarkably reduced the number of WBCs (Figure 1B ). This result was consistent with the fact that CTX could cause myelosuppression [ 5 ]. After CTX treatment, declines were found in the proliferation index of BMCs (4-d; Figure 1F ) and CD34 + SCA1 + BMHSCs ratio (Figure 1J ).…”

“…CTX frequently causes myelosuppression [ 5 ], and SHSB granule can cure myelosuppression without promoting the development of lung cancer [ 11 , 12 ]. However, its mechanism and therapeutic targets are still unclear.…”

“…Cyclophosphamide (CTX) has been widely used to block the progression of lung cancer in clinic [ 4 ]. However, CTX generally causes severe acute toxicities like myelosuppression [ 5 ]. Once myelosuppression appears, patients are more likely to suffer from infection.…”

Potato freeze‑thaw solution enhances immune function and antitumor activity in?vivo

“…These findings are compatible with a notion that dietary supplementation of purple sweet potato extract attenuates the suppression of T cell and B cell proliferation and T helper 1/T helper 2 cytokine imbalance in immunodeficient mice, partly contributing to ameliorate immune dysfunction (22). Collectively, it was speculated that stimulation of leukocyte proliferation or counteraction of bone marrow suppression may contribute to PFTS-mediated reversal of leukopenia by cyclophosphamide (23).…”

The complete mitochondrial genome sequence of Senna occidentalis (Fabales: Fabaceae)

“…The genus Senna of the Fabaceae family includes about 260 species in South America (Marazzi et al 2006). Senna occidentalis (formerly Cassia occidentalis) tissues are used in many countries as anti-inflammatory, antiplatelet, muscle relaxant, antimalaria, and antihemolytic medicines (Neboh and Ufelle 2015). A sample for sequencing was obtained from the National Agrobiodiversity Center (voucher number: IT 175099; geographic coordinates: N 35 49 0 19 00 , E 127 8 0 56 00 ) in Jeonju, Korea.…”