Myeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing

Ponthier, Jérôme; Desvals, Maud; Franck, Thierry; Rebière, Geoffroy de la; Spalart, Marc; Palmer, Eric; Serteyn, Didier; Deleuze, Stéfan

doi:10.1016/j.jevs.2011.06.003

Cited by 10 publications

(28 citation statements)

References 36 publications

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“…MPO is released during cooling step of the equine freezing procedure and is linked to non‐sperm cells (NSC) (Ponthier et al. , ). MPO is naturally glycosylated, thus explaining various molecular weights (120–150 kDa) (Taurog and Dorris ; Mathy‐Hartert et al.…”

Section: Introductionmentioning

confidence: 99%

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Concentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post‐Thaw Equine Semen and their Implication on Freezability

Ponthier

Franck

Parrilla-Hernandez

et al. 2014

Reprod Domestic Animals

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Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western blot. Purified active MPO was added in saline solution and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal-Wallis test, and correlations were determined using Spearman's test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post-thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = -0.5576, p = 0.0086). Inactive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post-thaw loss of activity is partially explained by MPO inactivation in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor.

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Section: Introductionmentioning

confidence: 99%

“…), MPO is naturally abundant in equine semen and its concentration is even higher in poor post‐thaw quality semen (Ponthier et al. , , ). The relation between total MPO concentration in equine semen and its activity assayed by specific immmunological extraction followed by enzymatic detection (SIEFED) (Franck et al.…”

Section: Introductionmentioning

confidence: 99%

Concentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post‐Thaw Equine Semen and their Implication on Freezability

Ponthier

Franck

Parrilla-Hernandez

et al. 2014

Reprod Domestic Animals

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“…MPO has recently been associated with keratinized cells and with decreased postthaw motility in stallion semen [1]. TNFa can bind to two structurally distinct membrane receptors on target cells TNFR1 (also known as p55 and TNFRSF1A) or TNFR2 (also known as p75 and TNFRSF1B).In general, TNFa largely relies on TNFR1 for apoptosis and on TNFR2 for any function related to survival.…”

mentioning

confidence: 99%

Tissue-specific gene expression of the fertility-associated proteins ADAM2, CD52, CRISP1, EQ-12, SED1 and Spam1 in the stallion's reproductive tract

Poels

Šoštarić

Stout

2012

Journal of Equine Veterinary Science

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“…MPO has recently been associated with keratinized cells and with decreased postthaw motility in stallion semen [1]. In particular, the expression of EQ-12, CD52 and CRISP1 is concentrated in the epididymis.…”

mentioning

confidence: 99%

“…MPO has recently been associated with keratinized cells and with decreased postthaw motility in stallion semen [1]. MPO has recently been associated with keratinized cells and with decreased postthaw motility in stallion semen [1].…”

mentioning

confidence: 99%

Effect of different methods for sperm selection on cooled stallion semen

Ramires-Neto

Monteiro

Delfiol

et al. 2012

Journal of Equine Veterinary Science

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Myeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing

Cited by 10 publications

References 36 publications

Concentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post‐Thaw Equine Semen and their Implication on Freezability

Concentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post‐Thaw Equine Semen and their Implication on Freezability

Tissue-specific gene expression of the fertility-associated proteins ADAM2, CD52, CRISP1, EQ-12, SED1 and Spam1 in the stallion's reproductive tract

Effect of different methods for sperm selection on cooled stallion semen

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