Myeloperoxidase Immunohistochemistry as a Measure of Disease Activity in Ulcerative Colitis: Association With Ulcerative Colitis-Colorectal Cancer, Tumor Necrosis Factor Polymorphism and RUNX3 Methylation

Garrity‐Park, Megan; Loftus, Edward V.; Sandborn, William J.; Smyrk, Thomas C.

doi:10.1002/ibd.21681

Cited by 52 publications

(28 citation statements)

References 49 publications

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“…30 Myeloperoxidase also demonstrates some promise as a marker of response to therapy. 63,64 Regarding mucosal healing, this evolving definition may ultimately include evaluation of both endoscopic and histologic remission, although standardized definitions of histologic response and remission are necessary and will require validation.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Assessment of Histologic Disease Activity in Crohnʼs Disease

Mojtahed¹,

Khanna²,

Sandborn³

et al. 2014

Inflammatory Bowel Diseases

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Section: Summary and Perspectivesmentioning

confidence: 99%

Assessment of Histologic Disease Activity in Crohnʼs Disease

Mojtahed¹,

Khanna²,

Sandborn³

et al. 2014

Inflammatory Bowel Diseases

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“…More recently, a cohort study by Gupta et al reported a significant link between histological inflammation and progression towards high-grade dysplasia or CAC, with a RR of 2 (Gupta et al, 2007). Finally, Garrity-Park et al showed an association between myeloperoxydase immunochemistry, TNF-α polymorphism and RUNX3 methylation and CAC (Garrity-Park et al, 2011). In addition to these clinical data, experimental results contribute to clarify the underlying cellular or molecular mechanisms explaining the link between inflammation and colorectal carcinogenesis in UC patients.…”

Section: Chronic Inflammationmentioning

confidence: 97%

Ulcerative Colitis-Associated Colorectal Cancer Prevention by 5-Aminosalicylates: Current Status and Perspectives

Reimund¹,

Tavernier²,

Viennot³

et al. 2012

Ulcerative Colitis From Genetics to Complications

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Ulcerative Colitis from Genetics to Complications 150 hypothesis have emerged, resulting from fundamental research in molecular biology and pharmacology. This increased understanding of the putative pathway(s) by which 5-ASA may interfere with CAC development appears also as the starting point for optimising 5-ASA derivatives or identifying new compounds acting more specifically and/or being more efficient in preventing neoplastic transformation of the colonic epithelium in UC patients. These different points will be addressed more precisely in the three next parts. Frequency of colorectal cancer risk in ulcerative colitis and risk factors

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“…Based on the literature, the markers CD68 and CD163 were used to mark macrophages, myeloperoxidase (MPO) for neutrophils, and CD161 as well as CD244 for NK cells (34)(35)(36)(37). Slides were deparaffinized, hydrated in water and individual antigens stained using a DAKO Autostainer Plus.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Cells that were positive for MPO were considered neutrophils (35). A representative image of the total neutrophil accumulation across all groups is shown in Fig.…”

Section: Innate Immune Cell Trafficking In the Esophagusmentioning

confidence: 99%

Dietary Consumption of Black Raspberries or Their Anthocyanin Constituents Alters Innate Immune Cell Trafficking in Esophageal Cancer

Peiffer

Wang

Zimmerman³

et al. 2016

Cancer Immunology Research

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Freeze-dried black raspberries (BRB), their component anthocyanins (AC), and a metabolite of BRB ACs, protocatechuic acid (PCA), inhibit the development of esophageal cancer in rats induced by the carcinogen, N-nitrosomethylbenzylamine (NMBA). All three components reduce inflammation in the esophagus and in plasma. The present study determined the relation of changes in inflammatory markers to infiltration of innate immune cells into NMBA-treated esophagus. Rats were injected with NMBA (0.35 mg/kg) for 5 weeks while on control diet. Following NMBA treatment, rats were fed diets containing 6.1% BRB powder, an AC-rich fraction of BRBs (3.8 mmol/g), or 500 ppm PCA. At weeks 15, 25, and 35, inflammatory biomarker expression in the plasma and esophagus was quantified, and infiltration of immune cells in the esophagus was examined. At all three time points, BRB, AC, and PCA similarly affected cytokine production in the esophagus and plasma of NMBA-treated rats, relative to the NMBA-only control. These included decreased expression of the proinflammatory cytokine IL1b and increased expression of the anti-inflammatory cytokine IL10. Moreover, all three diets also increased the expression of IL12, a cytokine that activates both cytolytic natural killer and CD8 þ T cells. In addition, the three diets also decreased infiltration of both macrophages and neutrophils into the esophagus. Overall, our results suggest that another mechanism by which BRBs, ACs, and PCA inhibit NMBA-induced esophageal tumorigenesis is by altering cytokine expression and innate immune cell trafficking into tumor tissues.

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Myeloperoxidase Immunohistochemistry as a Measure of Disease Activity in Ulcerative Colitis: Association With Ulcerative Colitis-Colorectal Cancer, Tumor Necrosis Factor Polymorphism and RUNX3 Methylation

Abstract: HDA is less sensitive than IHC and may underestimate inflammatory cell populations associated with UC-CRC. The epigenetic/genetic associations related to elevated MPO staining in UC-CRC may offer new methods for risk stratification and adjunctive screening tools.

Cited by 52 publications

References 49 publications

Assessment of Histologic Disease Activity in Crohnʼs Disease

Assessment of Histologic Disease Activity in Crohnʼs Disease

Ulcerative Colitis-Associated Colorectal Cancer Prevention by 5-Aminosalicylates: Current Status and Perspectives

Dietary Consumption of Black Raspberries or Their Anthocyanin Constituents Alters Innate Immune Cell Trafficking in Esophageal Cancer

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