Myeloperoxidase and Glutamate-Cysteine Ligase Polymorphisms in Type 2 Diabetes Mellitus: A Preliminary Study / Polimorfizmi Mijeloperoksidaze I Glutamat-Cistein Ligaze U Diabetes Mellitusu Tipa 2: Preliminarna Studija

Abstract

SummaryBackground: Oxidative stress can accompany the physiopathology of diabetes mellitus. Myeloperoxidase and glutamate-cysteine ligase catalytic subunit are two important substances which are connected with the maintaining of the redox balance in the body. In this study, the association between diabetes, lipid levels, myeloperoxidase -463G/A, and the glutamate-cysteine ligase catalytic subunit -3506A/G gene polymorphisms have been investigated. Methods: Frequencies of genotypes and alleles have been assessed using PCR-RFLP techniques in 90 type 2 diabetic patients and 70 healthy controls. Results: We found an association between myeloperoxidase -463 G/A genotype distributions (p=0.023) and allele distributions (p=0.009) in the study groups. In addition, there is a significant difference for each of the genotype (p=0.014) and allele (p=0.007) distributions for the glutamate-cysteine ligase catalytic subunit -3506A/G polymorphism. Conclusions: Our study reveals the first data about the myeloperoxidase -463G/A and glutamate-cysteine ligase catalytic subunit -3506A/G gene polymorphisms for type 2 diabetes and we believe that this work will play a pioneering role for further studies.