Myeloid‑derived suppressor cells in bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease

Brajer-Luftmann, Beata; Nowicka, Agata; Kaczmarek, Mariusz; Grabicki, Marcin; Kuźnar-Kamińska, Barbara; Bromińska, Barbara; Sikora, Jan; Batura‐Gabryel, Halina

doi:10.20452/pamw.3718

Cited by 5 publications

(20 citation statements)

References 9 publications

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“…Our previous studies confirmed that MDSCs and DAMPs may be involved in the local inflammation in COPD and may aggravate inflammation together with the severity of bronchoconstriction [21, 22]. In the current study, we attempted to find the relations among MDSCs and DAMPs in the lower airways, where the inflammatory process begins and persists, and in the serum, as the sign of the systemic inflammation.…”

Section: Discussionmentioning

confidence: 52%

“…The estimation of antigenic determinants, which are characteristic for MDSC populations, was performed using monoclonal antibodies directly conjugated with fluorochromes (Figure 1). MDSCs were defined as cells with immunophenotype SSC low /Lin-1 neg /HLA-DR neg/low /CD11 + /CD33 + /CD45 + [21]. The samples for cytofluorimetric analyses were prepared in the following manner.…”

Section: Methodsmentioning

confidence: 99%

“…Up to 50,000 events of each sample were collected. The percentage of positive cells was assessed [21].…”

Section: Methodsmentioning

confidence: 99%

“…Smoking history may not affect the number of MDSCs. These cells can be involved in the local inflammatory process in COPD, independently from smoking [21]. On the contrary, DAMPs may also be included in local and systemic inflammatory process in COPD.…”

Section: Introductionmentioning

confidence: 99%

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Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients

Brajer-Luftmann

Nowicka

Kaczmarek

et al. 2019

Journal of Immunology Research

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Myeloid-derived suppressor cells (MDSCs) are present in the human lung microenvironment, and they may be involved in the local inflammatory process in chronic obstructive pulmonary disease (COPD). Chronic inflammation in COPD may induce immunogenic cell death of structural airway cells, causing the release of damage-associated molecular patterns (DAMPs). DAMPs may activate the innate and adaptive immune system. The relationship between MDSCs and DAMPs in COPD is poorly described in the available literature. Objectives. (1) Assessment of MDSC percentage and DAMP concentration in bronchoalveolar lavage fluid (BALF) and peripheral blood. (2) Analysis of the relationship between MDSC percentage and chosen DAMPs. Patients and Methods. 30 COPD patients were included. Using monoclonal antibodies directly conjugated with fluorochromes in flow cytometry, MDSCs were assessed in BALF and peripheral blood. The concentration of DAMPs was estimated using sandwich ELISA. Using the Bradford method, the total protein concentrations were evaluated. Results. The percentage of MDSCs among MC in BALF correlated well with the concentration of defensin and heat shock protein 27. Assessing the percentage of MDSCs among all leukocytes in BALF, we revealed a significant correlation with the concentration of defensin, hyaluronic acid, and surfactant protein A. No dependencies occurred between DAMPs and MDSCs in peripheral blood. Conclusion. MDSCs and DAMPs occur in the COPD patient lung microenvironment. Significant correlations between them found in BALF may indicate their influence on the local inflammatory process in COPD. These relationships allow better understanding of the inflammatory process in COPD.

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Section: Discussionmentioning

confidence: 52%

Section: Methodsmentioning

confidence: 99%

“…Up to 50,000 events of each sample were collected. The percentage of positive cells was assessed [21].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients

Brajer-Luftmann

Nowicka

Kaczmarek

et al. 2019

Journal of Immunology Research

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“…61 Additionally, myeloid derived suppres sor cells may affect T cells and regulate inflam mation and carcinogenesis. 62 Other cells that participate in tumorigene sis include macrophages. During tumor progres sion, macrophages are recruited to the site of can cer by a wide spectrum of chemoattractants, de rived from the tumor and surrounding stroma.…”

Section: Review Articlementioning

confidence: 99%

Lung cancer in chronic obstructive pulmonary disease patients. Does cellular senescence matter?

Kuźnar-Kamińska¹,

Mikuła-Pietrasik²,

Książek³

et al. 2018

Polish Archives of Internal Medicine

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Neutrophils in chronic inflammatory diseases

2022

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Chronic inflammation is a component of many disease conditions that affect a large group of individuals worldwide. Chronic inflammation is characterized by persistent, low-grade inflammation and is increased in the aging population. Neutrophils are normally the first responders to acute inflammation and contribute to the resolution of inflammation. However, in chronic inflammation, the role of neutrophils is less well understood and has been described as either beneficial or detrimental, causing tissue damage and enhancing the immune response. Emerging evidence suggests that neutrophils are important players in several chronic diseases, such as atherosclerosis, diabetes mellitus, nonalcoholic fatty liver disease and autoimmune disorders. This review will highlight the interaction of neutrophils with other cells in the context of chronic inflammation, the contribution of neutrophils to selected chronic inflammatory diseases, and possible future therapeutic strategies.

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Myeloid‑derived suppressor cells in bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease

Cited by 5 publications

References 9 publications

Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients

Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients

Lung cancer in chronic obstructive pulmonary disease patients. Does cellular senescence matter?

Neutrophils in chronic inflammatory diseases

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