Myeloid and lymphoid activation markers in AIDS and non-AIDS presenters

Iannetta, Marco; Savinelli, Stefano; Rossi, Roberto; Mascia, Claudia; Marocco, Raffaella; Vita, Serena; Zuccalà, Paola; Zingaropoli, Maria Antonella; Mengoni, Fabio; Massetti, Anna Paola; Falciano, Mario; d’Ettorre, Gabriella; Ciardi, Maria Rosa; Mastroianni, Claudio Maria; Vullo, Vincenzo; Lichtner, Miriam

doi:10.1016/j.imbio.2018.11.011

Cited by 7 publications

(7 citation statements)

References 45 publications

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“…Contrary to classical monocytes, the proportion of HLA-DR + Lin 1 − cells in HIV-infected huNOG-EXL mice increased in blood along infection time (Figure 7B, left and middle panels), but decreased in SLO (Figure 7B, right panel), consistent with an egress of these cells to peripheral blood with infection in an attempt to restore the mature DC pool, or transitory migration to inflamed tissues [61]. Nonetheless, similar to previous human reports [62,63], we observed decreased frequencies of circulating pDCs and conserved frequencies of this subset in SLO (Figure 7C). There were no differences in the frequencies of mDCs between infected and uninfected huNOG-EXL mice (data not shown).…”

Section: Resultssupporting

confidence: 87%

HIV Replication in Humanized IL-3/GM-CSF-Transgenic NOG Mice

et al. 2019

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The development of mouse models that mimic the kinetics of Human Immunodeficiency Virus (HIV) infection is critical for the understanding of the pathogenesis of disease and for the design of novel therapeutic strategies. Here, we describe the dynamics of HIV infection in humanized NOD/Shi-scid-IL2rγnull (NOG) mice bearing the human genes for interleukin (IL)-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) (NOG-EXL mice). The kinetics of viral load, as well as the frequencies of T-cells, B-cells, Natural killer cells (NK), monocytes, and dendritic cells in blood and secondary lymphoid organs were evaluated throughout the time of infection. In comparison with a non-transgenic humanized mouse (NSG) strain, lymphoid and myeloid populations were more efficiently engrafted in humanized NOG-EXL mice, both in peripheral blood and lymphoid tissues. In addition, HIV actively replicated in humanized NOG-EXL mice, and infection induced a decrease in the percentage of CD4+ T-cells, inversion of the CD4:CD8 ratio, and changes in some cell populations, such as monocytes and dendritic cells, that recapitulated those found in human natural infection. Thus, the humanized IL-3/GM-CSF-transgenic NOG mouse model is suitable for the study of the dynamics of HIV infection and provides a tool for basic and preclinical studies.

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Section: Resultssupporting

confidence: 87%

HIV Replication in Humanized IL-3/GM-CSF-Transgenic NOG Mice

et al. 2019

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“…Circulating slan + monocytes produce higher TNF-α amounts than other monocyte subpopulations, and higher TNF-α amounts in HIV-1 viremic than in non-viremic people (13). Slan + monocytes accumulate in viremic people (13) as well as in cART virosuppressed people who have experienced low CD4 count nadirs (14), but they are depleted during AIDS (17). They are known to be present in the lesions of major inflammatory chronic diseases like Crohn's Abbreviations: cDC, conventional dendritic cells (type 1, cDC1; type 2, cDC2); EFS, Etablissement Français du Sang (French Blood Bank); GeoHD, healthy donors matched for geographical origin with PLWHIV-2 from the LTNP and P groups; HD, healthy donors (the abbreviation in the text is for healthy donors from the blood bank (EFS); LBP, Lipopolysaccharide-binding protein; LTNP, longterm non-progressors; P, progressors; PLWHIV, people living with HIV; WA, West-African.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In PLWHIV-1, sCD163 plasma levels were found to be higher than in controls, to correlate positively with viral loads as well as with CD14 + CD16 + monocyte counts, and inversely with the expression of CD163 on these cells and with CD4 + T cell counts. Also, sCD163 represents a marker of neurocognitive impairment or atheromatous plaque formation in people living with HIV ( 17 , 31 – 33 ). LPS-binding protein (LBP), an acute-phase reactant secreted by epithelial intestinal or hepatic cells in response to inflammation, mediates binding of the Lipid A part of LPS to CD14.…”

Section: Introductionmentioning

confidence: 99%

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Conventional Dendritic Cells and Slan+ Monocytes During HIV-2 Infection

et al. 2020

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HIV-2 infection is characterized by low viremia and slow disease progression as compared to HIV-1 infection. Circulating CD14 ++ CD16 + monocytes were found to accumulate and CD11c + conventional dendritic cells (cDC) to be depleted in a Portuguese cohort of people living with HIV-2 (PLWHIV-2), compared to blood bank healthy donors (HD). We studied more precisely classical monocytes; CD16 + inflammatory (intermediate, non-classical and slan + monocytes, known to accumulate during viremic HIV-1 infection); cDC1, important for cross-presentation, and cDC2, both depleted during HIV-1 infection. We analyzed by flow cytometry these PBMC subsets from Paris area residents: 29 asymptomatic, untreated PLWHIV-2 from the IMMUNOVIR-2 study, part of the ANRS-CO5 HIV-2 cohort: 19 long-term non-progressors (LTNP; infection ≥8 years, undetectable viral load, stable CD4 counts≥500/µL; 17 of West-African origin-WA), and 10 non-LTNP (P; progressive infection; 9 WA); and 30 age-and sex-matched controls: 16 blood bank HD with unknown geographical origin, and 10 HD of WA origin (GeoHD). We measured plasma bacterial translocation markers by ELISA. Non-classical monocyte counts were higher in GeoHD than in HD (54 vs. 32 cells/µL, p = 0.0002). Slan + monocyte counts were twice as high in GeoHD than in HD (WA: 28 vs. 13 cells/µL, p = 0.0002). Thus cell counts were compared only between participants of WA origin. They were similar in LTNP, P and GeoHD, indicating that there were no HIV-2 related differences. cDC counts did not show major differences between the groups. Interestingly, inflammatory monocyte counts correlated with plasma sCD14 and LBP only in PLWHIV-2, especially LTNP, and not in GeoHD. In conclusion, in LTNP PLWHIV-2, inflammatory monocyte counts correlated with LBP or sCD14 plasma levels, indicating a potential innate immune response to subclinical bacterial translocation. As GeoHD had higher inflammatory monocyte counts than HD, our data also show that specific controls are important to refine innate immunity studies.

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“…An additional subset of DCs, called slanDCs, was identified by the use of M-DC8 monoclonal antibody, directed against the 6-sulfo LacNAc1 -slan- carbohydrate modification of P -selectin glycoprotein ligand-1. These cells are highly proficient in secreting inflammatory cytokines in both autoimmunity and infections ( Schäkel et al, 1998 ; Dutertre et al, 2012 ; Günther et al, 2012 ; Hänsel et al, 2011 , 2013 ; Tufa et al, 2014 , 2016 ; Micheletti et al, 2016 ; Iannetta et al, 2019 ).…”

Section: Other Cells Of Innate Immunity: Dendritic Cells (Dcs) Monocytes Macrophages Neutrophils and Myeloid Derived Suppressor Cells (Mdmentioning

confidence: 99%

Immunopathology and Immunosenescence, the Immunological Key Words of Severe COVID-19. Is There a Role for Stem Cell Transplantation?

Ligotti

Pojero

Accardi

et al. 2021

Front. Cell Dev. Biol.

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The outcomes of Coronavirus disease-2019 (COVID-19) vary depending on the age, health status and sex of an individual, ranging from asymptomatic to lethal. From an immunologic viewpoint, the final severe lung damage observed in COVID-19 should be caused by cytokine storm, driven mainly by interleukin-6 and other pro-inflammatory cytokines. However, which immunopathogenic status precedes this “cytokine storm” and why the male older population is more severely affected, are currently unanswered questions. The aging of the immune system, i.e., immunosenescence, closely associated with a low-grade inflammatory status called “inflammageing,” should play a key role. The remodeling of both innate and adaptive immune response observed with aging can partly explain the age gradient in severity and mortality of COVID-19. This review discusses how aging impacts the immune response to the virus, focusing on possible strategies to rejuvenate the immune system with stem cell-based therapies. Indeed, due to immunomodulatory and anti-inflammatory properties, multipotent mesenchymal stem cells (MSCs) are a worth-considering option against COVID-19 adverse outcomes.

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Myeloid and lymphoid activation markers in AIDS and non-AIDS presenters

Cited by 7 publications

References 45 publications

HIV Replication in Humanized IL-3/GM-CSF-Transgenic NOG Mice

HIV Replication in Humanized IL-3/GM-CSF-Transgenic NOG Mice

Conventional Dendritic Cells and Slan+ Monocytes During HIV-2 Infection

Immunopathology and Immunosenescence, the Immunological Key Words of Severe COVID-19. Is There a Role for Stem Cell Transplantation?

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