Myelodysplastic syndrome in a case of new‐onset pancytopenia

Lancaster, Ian; Patel, Deep; Sethi, Vikas; Connelly, Weston; Namey, Joseph

doi:10.1002/ccr3.5533

Cited by 4 publications

(2 citation statements)

References 24 publications

(69 reference statements)

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“…Table 1b, Suppl. Table 2) 29,30 . Samples came from different studies but were processed in the same laboratory and with the same staff to avoid technical differences 31 .…”

Section: Methodsmentioning

confidence: 99%

Blood transcriptomics analysis offers insights into variant-specific immune response to SARS-CoV-2

Hoffmann,

Willruth,

Dietrich

et al. 2023

Preprint

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Bulk RNA sequencing (RNA-seq) of blood is typically used for gene expression analysis in biomedical research but is still rarely used in clinical practice. In this study, we argue that RNA-seq should be considered a routine diagnostic tool, as it offers not only insights into aberrant gene expression and splicing but also delivers additional readouts on immune cell type composition as well as B-cell and T-cell receptor (BCR/TCR) repertoires. We demonstrate that RNA-seq offers vital insights into a patient’s immune status via integrative analysis of RNA-seq data from patients infected with various SARS-CoV-2 variants (in total 240 samples with up to 200 million reads sequencing depth). We compare the results of computational cell-type deconvolution methods (e.g., MCP-counter, xCell, EPIC, quanTIseq) to complete blood count data, the current gold standard in clinical practice. We observe varying levels of lymphocyte depletion and significant differences in neutrophil levels between SARS-CoV-2 variants. Additionally, we identify B and T cell receptor (BCR/TCR) sequences using the tools MiXCR and TRUST4 to show that - combined with sequence alignments and pBLAST - they could be used to classify a patient’s disease. Finally, we investigated the sequencing depth required for such analyses and concluded that 10 million reads per sample is sufficient. In conclusion, our study reveals that computational cell-type deconvolution and BCR/TCR methods using bulk RNA-seq analyses can supplement missing CBC data and offer insights into immune responses, disease severity, and pathogen-specific immunity, all achievable with a sequencing depth of 10 million reads per sample.Key PointsComputational deconvolution of transcriptomes can estimate immune cell abundances in SARS-CoV-2 patients, supplementing missing CBC data.10 million RNA sequencing reads per sample suffice for analyzing immune responses and disease severity, including BCR/TCR identification.

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“…Table 1b, Suppl. Table 2) 29,30 . Samples came from different studies but were processed in the same laboratory and with the same staff to avoid technical differences 31 .…”

Section: Methodsmentioning

confidence: 99%

Blood transcriptomics analysis offers insights into variant-specific immune response to SARS-CoV-2

Hoffmann,

Willruth,

Dietrich

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Pancytopenia is a hematological condition defined as a decrease in three peripheral blood cell lines: leukopenia, thrombocytopenia, and anemia [ 6 ]. Based on previous case reports, the mechanism underlying the association between brucellosis and pancytopenia remains unclear.…”

Section: Introductionmentioning

confidence: 99%