Myelin basic protein‐positive nerve fibres in human Meissner corpuscles

García‐Suárez, Olivia; Montano, J.A; Esteban, I.; González-Martínez, T.; Álvarez-Abad, Covadonga; López-Arranz, E.; Cobo, Juan; Vega, J.A.

doi:10.1111/j.1469-7580.2009.01078.x

Cited by 22 publications

(21 citation statements)

References 26 publications

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“…It is generally accepted that Schwann cells lose the ability to myelinate as axons enter the receptor corpuscle; however, recent studies in human18 and primate digits19 report MBP immunoreactivity within the corpuscle. Approximately 25% of the MCs from human digital skin contained MBP‐immunoreactive nerve endings 10. Even so, they rarely appear to reach the apex of the papillae and purportedly do not actually supply the corpuscle.…”

Section: Cutaneous Neuroanatomymentioning

confidence: 99%

“…In comparison, cutaneous myelinated fibers have been much less explored, although numerous neuropathies are known to involve such distal myelinated nerve fibers. Recent studies have begun evaluating dermal myelinated fibers from glabrous skin with a variety of methods in healthy control populations9–11 and in patients with peripheral neuropathies 12–16. In this review, we will discuss the development of skin biopsy for investigation of normal and diseased dermal myelinated nerves.…”

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confidence: 99%

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Evaluating dermal myelinated nerve fibers in skin biopsy

Myers¹,

Peltier²,

Li³

2012

Muscle and Nerve

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Although there has been extensive research on small, unmyelinated fibers in the skin, little research has investigated dermal myelinated fibers in comparison. Glabrous, non-hairy skin contains mechanoreceptors that afford a vantage point for observation of myelinated fibers that have previously been seen only with invasively obtained nerve biopsies. This review discusses current morphometric and molecular expression data of normative and pathogenic glabrous skin obtained by various processing and analysis methods for cutaneous myelinated fibers. Recent publications have shed light on the role of glabrous skin biopsy in identifying signs of peripheral neuropathy and as a potential biomarker of distal myelin and mechanoreceptor integrity. The clinical relevance of a better understanding of the role of dermal myelinated nerve terminations in peripheral neuropathy will be addressed in light of recent publications in the growing field of skin biopsy.

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Section: Cutaneous Neuroanatomymentioning

confidence: 99%

mentioning

confidence: 99%

Evaluating dermal myelinated nerve fibers in skin biopsy

Myers¹,

Peltier²,

Li³

2012

Muscle and Nerve

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“…Previous studies have reported several SC-specific markers, and the most commonly used are S100 [ 2 ], MBP [ 1 , 3 ], MPZ [ 4 , 5 ], P75 NTR [ 6 , 7 ], GFAP [ 6 , 8 ], NCAM [ 5 , 9 ], GAP43 [ 6 , 10 ], PMP22 [ 11 ], Sox10 [ 12 ], Oct6 [ 13 ], O4 [ 14 ], Krox20 [ 15 ] and Sox2 [ 5 ]. Among them, S100 and P75 NTR are the most frequently used markers for the identification of stem cell—derived Schwann cell—like cells [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…A previous study suggested that Sox10 may be the only known marker constitutively expressed in the whole SC development process [ 19 , 23 ], while S100, the widely used SC marker, is not expressed in SCPs [ 24 ]. In addition, when iSCs differentiate into mSCs, transcription factor Oct6 is first expressed in pro-mSCs, inducing the expression of downstream transcription factor Krox20, which in turn regulates the expression of myelin-associated genes MBP and MPZ [ 3 , 10 , 15 , 25 – 27 ]. Another study suggested that Sox2, the marker for undifferentiated stem cells, is only expressed in SCPs and iSCs [ 5 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Specific Marker Expression and Cell State of Schwann Cells during Culture In Vitro

et al. 2015

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Schwann cells (SCs) in animals exist in different developmental stages or wound repair phases, distinguished mainly by the expression of SC-specific markers. No study has yet determined SC state under in vitro culture conditions, and the specific markers expressed in SC are obscure as well. In this study, we harvested sciatic nerves from newborn mice and isolated SCs by an enzyme-digestion method, then we examined the expression profiles of ten markers (S100, p75NTR, Sox10, Sox2, GAP43, NCAM, Krox20, Oct6, MBP, and MPZ) at both the RNA and protein levels in in vitro mouse SCs and speculated their relation with in vivo SC stages. We assayed RNA and protein levels of SC specific markers by immunofluorescence, Western Blot, and real-time quantitative RT-PCR. The results show that the expression of most markers (S100, p75NTR, GAP43, NCAM, Krox20, Oct6, MBP and MPZ) was not detectable in all of early stage cultured SCs. The expression of transcription factors Sox10 and Sox2 was, however, detectable in all SCs. After 8 days, the positive expression rate of all markers except GAP43 and Oct6 was almost 100%.These results indicates Sox10 is a necessary marker for SC identification, while S100 is not reliable. SCs cultured in vitro express Sox2, P75NTR, NCAM, GAP43, Oct6, and MPZ, suggesting that they are similar to in vivo undifferentiated iSCs or dedifferentiated iSCs after nerve injury.

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“…Two mechanisms – growth along the endoneurial tube and direct axon growth – might be responsible for the regeneration of Meissner’s corpuscles. The process of regenerated Meissner’s corpuscle development includes the growth of axons inside the end of corpuscles along the endoneurial tube or repeated sprouting, being packaged by theca cells, and development into the corpuscle body [22,23]. At the early stage, the axons at the terminal part of Meissner’s corpuscles were un-myelinated.…”

Section: Discussionmentioning

confidence: 99%

Autologous nerve implantation into denervated monkey skin promotes regeneration of Meissner’s corpuscle

et al. 2011

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SummaryBackgroundThe aim of this study was to observe the effects of autologous nerve implantation into the denervated finger flap on the regression and regeneration of sensory nerve endings and Meissner’s corpuscles.Material/MethodsBilateral nerves of fingers were separated: one was removed and the other was implanted into the denervated finger in the implantation group. In the non-implantation group, both nerves were removed. The ventral skin of fingers was collected for immunohistochemistry and electron microscopy 3, 6, 9 and 12 months after surgery.ResultsThe nerve endings in the Meissner’s corpuscles began to degenerate 3 months after denervation. The elementary structure of Meissner’s corpuscles was not significantly altered. Nerve fibers were present around the Meissner’s corpuscles, accompanied by growing into its inward. The axons in the denervated nerve disappeared and the Meissner’s corpuscles began to atrophy at month 6. More regenerated nerve fibers were observed after nerve implantation, including intensive and thick fibers, accompanied by reinnervation of Meissner’s corpuscles. More nerve fibers and a higher proportion of myelinated nerve fibers were noted at month 9 in the implantation group, and the reinnervation was present in the majority of Meissner’s corpuscles. Naive myelinated nerve fibers appeared at the caudal end of Meissner’s corpuscles. The nerve fibers in the Meissner’s corpuscles increased to the normal level at 12 months after nerve implantation.ConclusionsThe implanted nerve regenerated a large amount of free nerve endings, which helped to regenerate simple Meissner’s corpuscles via governing previously degenerated corpuscles.

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Myelin basic protein‐positive nerve fibres in human Meissner corpuscles

Cited by 22 publications

References 26 publications

Evaluating dermal myelinated nerve fibers in skin biopsy

Evaluating dermal myelinated nerve fibers in skin biopsy

Specific Marker Expression and Cell State of Schwann Cells during Culture In Vitro

Autologous nerve implantation into denervated monkey skin promotes regeneration of Meissner’s corpuscle

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