Mycotoxins in food and feed: toxicity, preventive challenges, and advanced detection techniques for associated diseases

Dey, Debasish Kumar; Kang, Ja Heon; Bajpai, Vivek K.; Kim, Kwanwoo; Lee, Hoomin; Sonwal, Sonam; Simal-Gándara, Jesús; Xiao, Jianbo; Ali, Sajad; Huh, Yun Suk; Han, Yong-Kyu; Shukla̽, Shruti

doi:10.1080/10408398.2022.2059650

Cited by 47 publications

(23 citation statements)

References 166 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…JAK (Janus tyrosine kinase) belongs to a family of non-receptor protein tyrosine kinases, comprising of JAK1, JAK2, JAK3, and TYK2 (non-receptor protein tyrosine kinase-2). The JNK signaling pathway mediated caspase-dependent programmed cell death machinery through IRE1α (Inositol-requiring enzyme-1α) and ERK (extracellular regulated protein kinases) signaling molecules [ 10 ]. It was reported that AFB 1 exposure could substantially increase endogenous ROS, which could activate the IRE1α/ERK1/2 signaling pathway and induce cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…AFB 1 stimulates cell membrane phospholipid A2 to cause lipid peroxidation, and induces the production of reactive oxygen free radicals (ROS), which could induce DNA to form 8-OHdG resulting in cell damage [ 8 , 9 ]. Low doses of ROS can be neutralized by phase 2 metabolic enzyme (glutathione S-transferases); excessive ROS could induce the endoplasmic reticulum oxidative stress, the increase of inflammatory factors, mitochondrial apoptosis, and other reactions [ 10 ]. The cytokines exist as markers of inflammatory response during the whole cell injury cycle [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Compound Mycotoxin Detoxifier on Alleviating Aflatoxin B1-Induced Inflammatory Responses in Intestine, Liver and Kidney of Broilers

Guo

Wang

Liu

et al. 2022

Toxins

View full text Add to dashboard Cite

In order to alleviate the toxic effects of aflatoxins B1 (AFB1) on inflammatory responses in the intestine, liver, and kidney of broilers, the aflatoxin B1-degrading enzyme, montmorillonite, and compound probiotics were selected and combined to make a triple-action compound mycotoxin detoxifier (CMD). The feeding experiment was divided into two stages. In the early feeding stage (1–21 day), a total of 200 one-day-old Ross broilers were randomly divided into four groups; in the later feeding stage (22–42 day), 160 broilers aged at 22 days were assigned to four groups: Group A: basal diet (4.31 μg/kg AFB1); Group B: basal diet with 40 μg/kg AFB1; Group C: Group A plus 1.5 g/kg CMD; Group D: Group B plus 1.5 g/kg CMD. After the feeding experiment, the intestine, liver, and kidney tissues of the broilers were selected to investigate the molecular mechanism for CMD to alleviate the tissue damages. Analyses of mRNA abundances and western blotting (WB) of inflammatory factors, as well as immunohistochemical (IHC) staining of intestine, liver, and kidney tissues showed that AFB1 aggravated the inflammatory responses through NF-κB and TN-α signaling pathways via TLR pattern receptors, while the addition of CMD significantly inhibited the inflammatory responses. Phylogenetic investigation showed that AFB1 significantly increased interleukin-1 receptor-associated kinase (IRAK-1) and mitogen-activated protein kinase (MAPK) activities (p < 0.05), which were restored to normal levels by CMD addition, indicating that CMD could alleviate cell inflammatory damages induced by AFB1.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Compound Mycotoxin Detoxifier on Alleviating Aflatoxin B1-Induced Inflammatory Responses in Intestine, Liver and Kidney of Broilers

Guo

Wang

Liu

et al. 2022

Toxins

View full text Add to dashboard Cite

show abstract

“…Microorganisms, especially ascomycetous fungi, are often found in contaminated agricultural products like food crops including corns, grain, fruits, and vegetables as well as poorly conserved foods. They produce diverse mycotoxins which are hazardous to human and animal health (Dey et al 2022 ; Nan et al 2022 ; Pallares et al 2022 ). On the other hand, the microbial natural products including mycotoxins are important drug candidates (Newman 2021 ) and the enzymes involved in the biosynthesis of these compounds are valuable biocatalysts for structural modification and construction of new biosynthetic pathways (Yi et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Diprenylated cyclodipeptide production by changing the prenylation sequence of the nature’s synthetic machinery

Coby

Zhou

et al. 2022

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Ascomycetous fungi are often found in agricultural products and foods as contaminants. They produce hazardous mycotoxins for human and animals. On the other hand, the fungal metabolites including mycotoxins are important drug candidates and the enzymes involved in the biosynthesis of these compounds are valuable biocatalysts for production of designed compounds. One of the enzyme groups are members of the dimethylallyl tryptophan synthase superfamily, which mainly catalyze prenylations of tryptophan and tryptophan-containing cyclodipeptides (CDPs). Decoration of CDPs in the biosynthesis of multiple prenylated metabolites in nature is usually initiated by regiospecific C2-prenylation at the indole ring, followed by second and third ones as well as by other modifications. However, the strict substrate specificity can prohibit the further prenylation of unnatural C2-prenylated compounds. To overcome this, we firstly obtained C4-, C5-, C6-, and C7-prenylated cyclo-l-Trp-l-Pro. These products were then used as substrates for the promiscuous C2-prenyltransferase EchPT1, which normally uses the unprenylated CDPs as substrates. Four unnatural diprenylated cyclo-l-Trp-l-Pro including the unique unexpected N1,C6-diprenylated derivative with significant yields were obtained in this way. Our study provides an excellent example for increasing structural diversity by reprogramming the reaction orders of natural biosynthetic pathways. Furthermore, this is the first report that EchPT1 can also catalyze N1-prenylation at the indole ring. Key points • Prenyltransferases as biocatalysts for unnatural substrates. • Chemoenzymatic synthesis of designed molecules. • A cyclodipeptide prenyltransferase as prenylating enzyme of already prenylated products. Graphical Abstract

show abstract

“…A recent study has reviewed that human dietary exposure to AFB1 and AFM1 is mainly via grains, grain-based products, and milk products [8]. While previous studies focused on individual toxic molecules, humans are more likely to be exposed to more than one contaminant in the environment [9].…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Reveal Additive Cytotoxicity Effects of Aflatoxin B1 and Aflatoxin M1 toward Intestinal NCM460 Cells

Gao

Yang

Wang

et al. 2022

Toxins

View full text Add to dashboard Cite

Aflatoxin B1 (AFB1) is a common crop contaminant, while aflatoxin M1 (AFM1) is implicated in milk safety. Humans are likely to be simultaneously exposed to AFB1 and AFM1; however, studies on the combined interactive effects of AFB1 and AFM1 are lacking. To fill this knowledge gap, transcriptomic, proteomic, and microRNA (miRNA)-sequencing approaches were used to investigate the toxic mechanisms underpinning combined AFB1 and AFM1 actions in vitro. Exposure to AFB1 (1.25–20 μM) and AFM1 (5–20 μM) for 48 h significantly decreased cell viability in the intestinal cell line, NCM460. Multi-omics analyses demonstrated that additive toxic effects were induced by combined AFB1 (2.5 μM) and AFM1 (2.5 μM) in NCM460 cells and were associated with p53 signaling pathway, a common pathway enriched by differentially expressed mRNAs/proteins/miRNAs. Specifically, based on p53 signaling, cross-omics showed that AFB1 and AFM1 reduced NCM460 cell viability via the hsa-miR-628-3p- and hsa-miR-217-5p-mediated regulation of cell surface death receptor (FAS), and also the hsa-miR-11-y-mediated regulation of cyclin dependent kinase 2 (CDK2). We provide new insights on biomarkers which reflect the cytotoxic effects of combined AFB1 and AFM1 toxicity.

show abstract

Mycotoxins in food and feed: toxicity, preventive challenges, and advanced detection techniques for associated diseases

Cited by 47 publications

References 166 publications

Effects of Compound Mycotoxin Detoxifier on Alleviating Aflatoxin B1-Induced Inflammatory Responses in Intestine, Liver and Kidney of Broilers

Effects of Compound Mycotoxin Detoxifier on Alleviating Aflatoxin B1-Induced Inflammatory Responses in Intestine, Liver and Kidney of Broilers

Diprenylated cyclodipeptide production by changing the prenylation sequence of the nature’s synthetic machinery

Multi-Omics Reveal Additive Cytotoxicity Effects of Aflatoxin B1 and Aflatoxin M1 toward Intestinal NCM460 Cells

Contact Info

Product

Resources

About