Mycotoxins and Other Secondary Metabolites Produced in Vitro by<i>Penicillium paneum</i>Frisvad and<i>Penicillium roqueforti</i>Thom Isolated from Baled Grass Silage in Ireland

O’Brien, Martin; Nielsen, Kristian Fog; O’Kiely, P.; Forristal, P. D.; Fuller, Hubert T.; Frisvad, Jens Christian

doi:10.1021/jf0621018

Cited by 110 publications

(85 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the techniques applied, we excluded the isolation of potential non-bioactive R. mobilis strains, it is, however, intriguing that the consistent production of TDA indicates a strong evolutionary pressure in their natural habitat to maintain the megaplasmid carrying the highly conserved TDA biosynthetic genes (Sule and Belas, 2013;Harrington et al, 2014). Similar observations have been made in very different niches, such as silage where Penicillium paneum and P. roqueforti produce marcfortines and mycophenolic acid, respectively, and share andrastins (O'Brien et al, 2006).…”

Section: Discussionmentioning

confidence: 71%

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

et al. 2016

View full text Add to dashboard Cite

Tropodithietic acid (TDA)-producing Ruegeria mobilis strains of the Roseobacter clade have primarily been isolated from marine aquaculture and have probiotic potential due to inhibition of fish pathogens. We hypothesized that TDA producers with additional novel features are present in the oceanic environment. We isolated 42 TDA-producing R. mobilis strains during a global marine research cruise. While highly similar on the 16S ribosomal RNA gene level (99-100% identity), the strains separated into four sub-clusters in a multilocus sequence analysis. They were further differentiated to the strain level by average nucleotide identity using pairwise genome comparison. The four sub-clusters could not be associated with a specific environmental niche, however, correlated with the pattern of sub-typing using co-isolated phages, the number of prophages in the genomes and the distribution in ocean provinces. Major genomic differences within the sub-clusters include prophages and toxin-antitoxin systems. In general, the genome of R. mobilis revealed adaptation to a particle-associated life style and querying TARA ocean data confirmed that R. mobilis is more abundant in the particle-associated fraction than in the free-living fraction occurring in 40% and 6% of the samples, respectively. Our data and the TARA data, although lacking sufficient data from the polar regions, demonstrate that R. mobilis is a globally distributed marine bacterial species found primarily in the upper open oceans. It has preserved key phenotypic behaviors such as the production of TDA, but contains diverse sub-clusters, which could provide new capabilities for utilization in aquaculture.

show abstract

Section: Discussionmentioning

confidence: 71%

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Among mycotoxins produced by Penicillium spp. are ochratoxins, patulin and citrinin (O'Brien et al, 2006), while A. niger produces, ochratoxin A and malformins among others (Frisvad, Smedsgaard, Samson, Larsen, & Thrane, 2007;Weidenbörner, 2008). Although Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

Changes in Fungal Population and Aflatoxin Levels and Assessment of Major Aflatoxin Types in Stored Peanuts (Arachis hypogaea Linnaeus)

Wagacha¹,

Mutegi²,

Christie³

et al. 2013

JFR

View full text Add to dashboard Cite

Peanut kernels of Homabay Local, Valencia Red, ICGV-SM 12991 and ICGV-SM 99568 cultivars were stored for six months in jute, polypropylene and polyethylene bags to assess the effect of storage bags, temperature and R.H. on fungal population and aflatoxin contamination. Moisture content (M.C.), fungal population and aflatoxin levels were determined before storage and after every 30 days during storage. Isolates of Aspergillus flavus and A. parasiticus were assayed for production of aflatoxin B1, B2, G1 and G2. The correlation between MC, population of A. flavus and A. parasiticus and aflatoxin levels in peanuts was also determined. Six fungal pathogens were commonly isolated from the peanut samples and occurred as follows in decreasing order: Penicillium spp. (106.6 CFU/g), A. flavus L-strain (4.8 CFU/g), A. flavus S-strain (2.9 CFU/g), A. niger (2.6 CFU/g), A. parasiticus (1.7 CFU/g) and A. tamarii (0.2 CFU/g). The overall population of A. flavus L-strain was 66% higher than that of A. flavus S-strain. Ninety one percent of A. flavus and A. parasiticus isolates produced at least one of the four aflatoxin types assayed, with 36% producing aflatoxin B1. Total aflatoxin levels ranged from 0 -47.8 µg/kg with samples stored in polyethylene and jute bags being the most and least contaminated, respectively. Eighty nine percent and 97% of the peanut samples met the EU (≤ 4 µg/kg) and Kenyan (≤ 10 µg/kg) regulatory standards for total aflatoxin, respectively. Peanuts should be adequately dried to safe moisture level and immediately packaged in a container -preferably jute bags -which will not promote critical increases in fungal population and aflatoxin contamination.

show abstract

“…(Schneweis et al 2001), and Byssochlamys nivea (Escoula 1975) as the most prevalent fungal species in silage. Mycotoxins produced by these fungi include patulin, mycophenolic acid, penicillic acid, roquefortins, marcfortine A, andrastin A, gliotoxin, and toxins of the verruculogen/fumitremorgen group (Garon et al 2006;O'Brien et al 2006). It should be mentioned that mycotoxins originating from pre-harvest contamination of forages that are ensiled are often unaffected by the ensiling process, and add to the overall mycotoxin contamination.…”

Section: Mycotoxins In Feeds For Dairy Cattlementioning

confidence: 99%

Mycotoxins in cattle feeds and carry-over to dairy milk: A review

Fink‐Gremmels

2008

Food Additives & Contaminants: Part A

309

260

View full text Add to dashboard Cite

The complex diet of ruminants, consisting of forages, concentrates, and preserved feeds, can be a source of very diverse mycotoxins that contaminate individual feed components. A number of mycotoxins are successfully inactivated by the rumen flora, whereas others pass unchanged or are converted into metabolites that retain biological activity. Hence, the barrier function of the rumen largely determines the susceptibility of dairy cows and other ruminant species towards individual mycotoxins. An impairment of this barrier function due to diseases or the direct antimicrobial effect of certain mycotoxins may increase absorption rates. The rate of absorption determines not only the internal dose and risk for adverse health effects, but also the excretion of mycotoxins and the biologically active metabolites into milk.

show abstract

Mycotoxins and Other Secondary Metabolites Produced in Vitro byPenicillium paneumFrisvad andPenicillium roquefortiThom Isolated from Baled Grass Silage in Ireland

Cited by 110 publications

References 36 publications

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

Changes in Fungal Population and Aflatoxin Levels and Assessment of Major Aflatoxin Types in Stored Peanuts (Arachis hypogaea Linnaeus)

Mycotoxins in cattle feeds and carry-over to dairy milk: A review

Contact Info

Product

Resources

About