Mycotic Keratitis due to Beauveria alba

Pj, McDonnell; Tp, Werblin; Sigler, Lynne; Wr, Green

doi:10.1097/00003226-198403000-00010

Cited by 24 publications

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“…The role of BEA in inhibiting inflammation was previously reported in CD-1 mice [7, 8]. As the relationship between inflammation and asthma are becoming better understood, the importance of anti-inflammatory treatment is increasingly accepted.…”

Section: Discussionmentioning

confidence: 90%

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Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis

2016

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The present study aimed to investigate the role of beauveria (BEA) in asthma. We investigated the cytotoxic effect of BEA on the proliferation of inflammatory cells and secretion of inflammatory mediators both in-vitro and in-vivo. In in-vitro studies, BEA inhibited lymphocytic cell proliferation and the proliferation of lymphocytic cells induced by Phorbol-12-myristate-13-acetate (PMA). We used ELISA to test the effects of BEA on the secretion of inflammatory factors including tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12) and interferon-gamma (IFN-γ). Flow cytometry was used to evaluate the influence of BEA on cell apoptosis. The effect of BEA on the cell numbers of eosinophils, lymphocytes, macrophages, neutrophils and other cells in mouse bronchoalveolar lavage fluid (BALF) was also evaluated. The expression of apoptosis related molecules Bax, Caspase-3 and Bcl-2 was examined by Western blotting analysis. Our results indicated that BEA played a protective role in asthma. BEA inhibited lymphocytic cell proliferation and secretion of inflammatory mediators. BEA promoted cell apoptosis, stimulated the expression of Bax and Caspase-3 and inhibited Bcl-2 protein expression in a dose-dependent manner. In in-vivo experiments, BEA reduced the cell number of eosinophils, lymphocytes, macrophages, neutrophils and other cells in mouse BALF. BEA inhibited secretion of inflammatory mediators, stimulated expression of Bax and Caspase-3, and inhibited expression of Bcl-2 in mouse lung tissue dose-dependently. All together, our results indicated that BEA could attenuate asthma by inhibiting inflammatory response and induce apoptosis of inflammatory cells.

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Section: Discussionmentioning

confidence: 90%

“…The role of BEA in inhibiting inflammation was previously reported in CD-1 mice [7, 8]. Many plant species containing flavonoids has been widely used as traditional Chinese medicine.…”

Section: Introductionmentioning

confidence: 96%

Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis

2016

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“…This is the second report in literature and the first in India, where in Engyodontium album has been isolated and confirmed in corneal scrapping. The first case of mycotic keratitis caused by same fungus following phacoemulsification and intraocular lens implantation was reported by McDonell et al (1984) [4] as Beaveria alba.…”

Section: Introductionmentioning

confidence: 99%

Mycotic keratitis due to Engyodontium album: First case report from India

Thamke

Mendiratta

Dhabarde

et al. 2015

Indian Journal of Medical Microbiology

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at the centre of the pupillary area of size 7 mm × 8 mm and fluorescent stain was positive. No superficial or deep vascularisation was seen. Corneal sensations were decreased at the centre of the papillary area. On anterior chamber examination, depth was normal and hypopyon was present. On direct ophthalmoscopy, the media was hazy and fundus details were not seen. Based on these findings, the diagnosis of hypopyon fungal corneal ulcer with endophthalmitis was made. Corneal scrapping was obtained by using Bard Parker knife no. 15 for direct microscopic examination and culture.Mycology work-up: A 10%-KOH mount showed hyaline septate hyphae [ Figure 1a]. Gram stain was negative for bacteria, and wet mount was negative for cysts of acanthamoeba. In Calcoflour white stain, apple green septate hyphae were seen. Corneal scrapings were inoculated on a plate of SDA with chloramphenicol and incubated at 25°C in BOD and on sheep blood agar at 37°C. On SDA plate, white floccose colonies were seen with colourless reverse within 3 days [ Figure 1b and c].Microscopic examination of slide culture of the white floccose colonies showed thin hyaline septate hyphae. Conidiophores were ascending bearing Abstract Engyodontium album is a rare and an unusual human pathogen. It is a common inhabitant of waste and moist material and frequently isolated from substrates such as paper, jute, linen and painted walls. This fungus grew within 3 days on SDA with chloramphenicol from corneal scrapping of a 70-year-old male farmer with a history of trauma by unknown vegetative matter. The fungus can be confused with Tritirachium sp and Beauveria sp.

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“…Limber (7) then included it in a new genus, Tritirachium, but since 1972 a new genus Engyodontium has been created which includes two species, E. album and E. parvisporum (3). Infections involving E. album include eczema vesiculosum (4), granulomatous skin lesions, brain abscess (9) and keratitis (8) Three venous blood samples were collected in consecutive days, aseptically by venipuncture into VACUTAINER® tubes using EDTA anticoagulant. They were processed by standard methods (direct examination and isolation in culture) for Direct examination was performed without clarifying and staining clinical samples.…”

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confidence: 99%

Engyodontium album fungaemia: the first reported case

Macêdo¹,

Neves²,

Souza-Motta³

et al. 2007

Braz. J. Microbiol.

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Opportunistic mycoses have been increasingly observed among immunocompromised patients. We describe a case in which Engyodontium album was isolated and cultured from the blood of a patient with the Acquired Immunodeficiency Syndrome. E. album grew at 37ºC and showed proteinase activity, both indicators of pathogenicity. This is the first time that this organism has been reported as agent of fungaemia.Key words: Engyodontium album, fungaemia, Acquired Immunodeficiency Syndrome Opportunistic mycoses have been increasingly observed in immuno-compromised patients. Engyodontium album is an unusual pathogen but a rather common inhabitant of waste or moist material and can be isolated from substrates such as paper, jute, linen, and painted walls. Its dispersal is by dry, hygrophobic conidia and can be isolated from house air (1).E. album used to be included in the genus Beauveria (10). Limber (7) then included it in a new genus, Tritirachium, but since 1972 a new genus Engyodontium has been created which includes two species, E. album and E. parvisporum (3). Infections involving E. album include eczema vesiculosum (4), granulomatous skin lesions, brain abscess (9) Direct examination was performed without clarifying and staining clinical samples. Subcultures were prepared using Sabouraud dextrose agar plus chloranphenicol (Difco Laboratories) incubated at 30ºC and 35ºC in an aerobic atmosphere for 15 days. Pure cultures were transferred onto the surface of potato dextrose agar medium to taxonomic identification (5,6).Preliminary pathogenicity tests were carried out through proteinase detection using casein and gelatin as substrates (6). The isolated fungus was inoculated on the referred culture medium, incubated at room temperature and at 37ºC in order to detect the formation of a transparent halo, and observed for 10 to 15 days. When a transparent zone occurred, the result was considered positive, regardless the diameter.Direct examination revealed septation of hyaline hyphae. Macroscopically the colonies were floccose, white, and 24 mm in diameter. The reverse of the colony was colorless. Microscopic examination showed narrow vegetative hyphae which were 1 to 2 µm wide, bearing fertile hyphae which were 2 to 4 µm wide and apically dichotomously branched, bearing conidiogenous cells in whorls of one to three. Conidiogenous cells consisted of an elongated cylindrical structure with a well-developed rachis with denticles. Conidia were hyaline, smooth, and globose (Fig. 1). The organism was identified as E. album according to the

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Mycotic Keratitis due to Beauveria alba

Cited by 24 publications

References 0 publications

Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis

Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis

Mycotic keratitis due to Engyodontium album: First case report from India

Engyodontium album fungaemia: the first reported case

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