Mycorrhizal synthesis between <i>Tuber borchii</i> and <i>Arbutus unedo</i> L. seedlings and micropropagated plants

Plácito, F.; Ferreira, Inês João; Clemente, M.; Figueiredo, P.; Barrento, Maria João; Machado, Helena; Gomes, Filomena

doi:10.17660/actahortic.2018.1224.13

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…As the main difference, in arbutoid mycorrhizae, fungal hyphae colonize the rhizodermal cells and fill them with hyphal coils (Massicotte et al 1993 ). Kennedy et al ( 2012 ) identified two Tuber species as fungal partners in arbutoid mycorrhizae formed with Arbutus menziesii Pursh, while Lancellotti et al ( 2014 ) and Plácito et al ( 2018 ) described arbutoid mycorrhizae between T. borchii and Arbutus unedo L. either recovered from the wild or synthetized in greenhouse. However, it needs to be demonstrated that this plant can be successfully used for large-scale programmes of truffle mycorrhization.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and ultrastructural observation of arbutoid mycorrhizae of black truffles (Tuber melanosporum and T. aestivum)

et al. 2020

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Arbutus unedo (the strawberry tree) is a Mediterranean shrub which forms arbutoid mycorrhizae with a variety of Asco- and Basidiomycetes. After the discovery of the mycorrhizal symbiosis between A. unedo and Tuber borchii, in this study, arbutoid mycorrhizae were synthetized in greenhouse with Tuber aestivum and Tuber melanosporum. Six months after inoculation, both species colonized the roots of all inoculated A. unedo seedlings, but mature mycorrhizae were only observed after 12 months. Ultrastructure analysis of Tuber arbutoid mycorrhizae was described for the first time, showing, as observed in typical endosymbiosis, a rearrangement of host cells and the creation of an interface compartment with both truffle species. Immunolabelling experiments suggested that pectins are not present in the interface matrix surrounding the intracellular hyphae. Thus, the ability to establish symbiosis with A. unedo seems to be a common feature in the genus Tuber, opening up the possibility to use this plant for mycorrhization with valuable truffles. This could represent an important economic opportunity in Mediterranean areas by combining the production of truffles, edible fruits and valued honey.

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Section: Introductionmentioning

confidence: 99%

Synthesis and ultrastructural observation of arbutoid mycorrhizae of black truffles (Tuber melanosporum and T. aestivum)

et al. 2020

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Effect of Quercetin on Mycorrhizal Synthesis between Tuberborchii and Arbutusunedo L. In Vitro Plants

Gomes

Castro

Santos

et al. 2021

Microbiology Research

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Arbutus unedo L. is a Mediterranean species used for fruit production; it is tolerant to drought and shows regeneration ability following forest fires. Mycorrhizal plants with Tuber borchii add resilience and value. This study aims to test the effect of quercetin on mycorrhizal synthesis between T. borchii and A. unedo. Two genotypes selected for fruit production and hydric stress tolerance, were micropropagated for mycorrhizal synthesis, accomplished during ex vitro rooting in perlite, using lyophilized spores of T. borchii suspended in culture media with different quercetin levels (0–10 µM). Six months after inoculation, plants were transferred to pots and maintained in nursery. Ten and 12 months after inoculation, roots were morphological examined and molecularly characterized using ITS1-5.8SITS2 rDNA region and specific primers. Results showed that mycorrhizae establishment was dependent on studied factors (genotype, quercetin level, and culture medium) and their interaction (genotype X culture medium). Quercetin levels up to 2.0 µM favored mycorrhizae establishment and plant growth, although levels superior to 4 µM showed a toxic effect. Quercetin showed to be an efficient factor on inducing mycorrhiza thriving independent of the genotype. Morphological observations and molecular analysis confirmed the permanence of the fungus association 10 and 12 months after inoculation.

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Mycorrhizal synthesis between Tuber borchii and Arbutus unedo L. seedlings and micropropagated plants

Cited by 2 publications

References 20 publications

Synthesis and ultrastructural observation of arbutoid mycorrhizae of black truffles (Tuber melanosporum and T. aestivum)

Synthesis and ultrastructural observation of arbutoid mycorrhizae of black truffles (Tuber melanosporum and T. aestivum)

Effect of Quercetin on Mycorrhizal Synthesis between Tuberborchii and Arbutusunedo L. In Vitro Plants

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