Mycorrhizal Fungi as Control Agents Against Plant Pathogens

Tripathi, Swati; Mishra, Siddhartha; Varma, Ajit

doi:10.1007/978-3-319-68867-1_8

Cited by 11 publications

(9 citation statements)

References 112 publications

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“…To date ~500 diverse siderophores have been documented [ 9 ]. A majority of the siderophores are reported to be endophytic in nature, being found in various microbial sources including mycorrhiza and orchidaceous fungi [ 8 , 10 , 11 ]. However, the nature of siderophores is different among microbes, for instance the bacterial hydroxamates are made up of hydroxylated and acylated alkylamines, while fungal hydroxamates are composed of hydroxylated and acylated ornithine groups.…”

Section: Introductionmentioning

confidence: 99%

Detection and Characterization of Antibacterial Siderophores Secreted by Endophytic Fungi from Cymbidium aloifolium

et al. 2020

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Endophytic fungi from orchid plants are reported to secrete secondary metabolites which include bioactive antimicrobial siderophores. In this study endophytic fungi capable of secreting siderophores were isolated from Cymbidium aloifolium, a medicinal orchid plant. The isolated extracellular siderophores from orchidaceous fungi act as chelating agents forming soluble complexes with Fe3+. The 60% endophytic fungi of Cymbidium aloifolium produced hydroxamate siderophore on CAS agar. The highest siderophore percentage was 57% in Penicillium chrysogenum (CAL1), 49% in Aspergillus sydowii (CAR12), 46% in Aspergillus terreus (CAR14) by CAS liquid assay. The optimum culture parameters for siderophore production were 30 °C, pH 6.5, maltose and ammonium nitrate and the highest resulting siderophore content was 73% in P. chrysogenum. The total protein content of solvent-purified siderophore increased four-fold compared with crude filtrate. The percent Fe3+ scavenged was detected by atomic absorption spectra analysis and the highest scavenging value was 83% by P. chrysogenum. Thin layer chromatography of purified P. chrysogenum siderophore showed a wine-colored spot with Rf value of 0.54. HPLC peaks with Rts of 10.5 and 12.5 min were obtained for iron-free and iron-bound P. chrysogenum siderophore, respectively. The iron-free P. chrysogenum siderophore revealed an exact mass-to-charge ratio (m/z) of 400.46 and iron-bound P. chrysogenum siderophore revealed a m/z of 453.35. The solvent-extracted siderophores inhibited the virulent plant pathogens Ralstonia solanacearum, that causes bacterial wilt in groundnut and Xanthomonas oryzae pv. oryzae which causes bacterial blight disease in rice. Thus, bioactive siderophore-producing endophytic P. chrysogenum can be exploited in the form of formulations for development of resistance against other phytopathogens in crop plants.

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Section: Introductionmentioning

confidence: 99%

Detection and Characterization of Antibacterial Siderophores Secreted by Endophytic Fungi from Cymbidium aloifolium

et al. 2020

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“…Studies have shown that AMF can improve plant stress resistance, such as saline-alkali resistance, heavy-metal resistance, drought resistance [10,11], and disease resistance (resistance to root rot, Verticillium wilt, blight, etc.) [12][13][14][15]. Guo et al found that after inoculation with F. mosseae, the symbiotic mycorrhizal network formed between F. mosseae and donor plants increased the activities of phenylalanine ammonialyase, polyphenol oxidase, and peroxidase in tobacco plants [16].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic analyses revealed the effect of Funneliformis mosseae on genes expression in Fusarium oxysporum

et al. 2020

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Soybean root rot is a typical soil-borne disease that severely affects the yield of soybean. Funneliformis mosseae is one of the arbuscular mycorrhizal fungi(AMF) dominant strains in soybean continuous cropping soil. The aim of this study was to providing an experimental basis for the study of the molecular mechanism underlying the alleviation of the obstacles associated with the continuous cropping of soybean by AMF. In this study, F. mosseae was inoculated in soil planted with soybean infected with Fusarium oxysporum. The results showed that the incidence of soybean root rot was significantly reduced after inoculation with F. mosseae. In F. mosseae-treated samples, the significantly upregulated genes encoded transmembrane protein in fungal cell membrane. The significantly downregulated genes encoded some proteins, which took part in composition of essential component of fungal cell wall; hydrolyse cellulose and hemicellulose. The DEGs in each treatment were enriched in antigen processing and presentation, carbon fixation in photosynthetic organisms, glycolysis/gluconeogenesis, the MAPK signalling pathway, protein processing in the endoplasmic reticulum and RNA degradation. Inoculation with F. mosseae could in a variety of ways to promote the growth, development of soybean and improve disease resistance. Such as help fungal build barriers to the disease resistance of host plant and enhance their pathogenicity; damaging the structure of the pathogen; protect plant tissues and so on. This study provides an experimental basis for further research on the molecular mechanism underlying the alleviation of challenges associated with the continuous cropping of soybean by AMF.

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“…Studies have shown that AMF can improve plant stress resistance, such as saline-alkali resistance, heavy-metal resistance, drought resistance [7][8], and disease resistance (resistance to root rot, Verticillium wilt, blight, etc.) [9][10][11][12]. Guo et al found that after inoculation with F. mosseae, the symbiotic mycorrhizal network formed between F. mosseae and donor plants increased the activities of phenylalanine ammonialyase, polyphenol oxidase, and peroxidase in tobacco plants [13].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analyses revealed the effect ofFunneliformis mosseae on differentially expressed genes inFusarium oxysporum

Zhang¹,

Bai

Guo

et al. 2020

Preprint

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Soybean root rot is a typical soil-borne disease that severely affects the yield of 29 soybean, and F. mosseae, the dominant strain of AMF in continuous cropping of soybean. The 30 aim of this study was to providing an experimental basis for the study of the molecular 31 mechanism underlying the alleviation of the obstacles associated with the continuous cropping 32 of soybean by AMF. In this study, F. mosseae was inoculated in soil planted with soybean 33 infected with F. oxysporum. The results showed that the incidence of soybean root rot was 34 significantly reduced after inoculation with F. mosseae. The significantly upregulated genes 35 encoded the ABC transporter, ATP-binding/permease protein and the ABC transporter, 36 ATP-binding protein. The significantly downregulated genes encoded chitin-binding domain 37 proteins; key enzymes involved in metabolic pathways such as glycolysis, including class II 38 fructose-bisphosphate aldolase and NAD-dependent glyceraldehyde-3-phosphate 39 dehydrogenase, glycoside hydrolase family 61 protein, which hydrolyse cellulose and 40 hemicellulose; actin and other major components of the cytoskeleton. The DEGs were enriched 41 in antigen processing and presentation, carbon fixation in photosynthetic organisms, 42 glycolysis/gluconeogenesis, the MAPK signalling pathway, protein processing in the 43 endoplasmic reticulum and RNA degradation. Inoculation with F. mosseae could promote the 44 growth and development of soybean and improve disease resistance. This study provides an 45experimental basis for further research on the molecular mechanism underlying the alleviation 46 of challenges associated with the continuous cropping of soybean by AMF. 47

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Mycorrhizal Fungi as Control Agents Against Plant Pathogens

Cited by 11 publications

References 112 publications

Detection and Characterization of Antibacterial Siderophores Secreted by Endophytic Fungi from Cymbidium aloifolium

Detection and Characterization of Antibacterial Siderophores Secreted by Endophytic Fungi from Cymbidium aloifolium

Transcriptomic analyses revealed the effect of Funneliformis mosseae on genes expression in Fusarium oxysporum

Transcriptomic analyses revealed the effect ofFunneliformis mosseae on differentially expressed genes inFusarium oxysporum

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