Soybean root rot is a typical soil-borne disease that severely affects the yield of 29 soybean, and F. mosseae, the dominant strain of AMF in continuous cropping of soybean. The 30 aim of this study was to providing an experimental basis for the study of the molecular 31 mechanism underlying the alleviation of the obstacles associated with the continuous cropping 32 of soybean by AMF. In this study, F. mosseae was inoculated in soil planted with soybean 33 infected with F. oxysporum. The results showed that the incidence of soybean root rot was 34 significantly reduced after inoculation with F. mosseae. The significantly upregulated genes 35 encoded the ABC transporter, ATP-binding/permease protein and the ABC transporter, 36 ATP-binding protein. The significantly downregulated genes encoded chitin-binding domain 37 proteins; key enzymes involved in metabolic pathways such as glycolysis, including class II 38 fructose-bisphosphate aldolase and NAD-dependent glyceraldehyde-3-phosphate 39 dehydrogenase, glycoside hydrolase family 61 protein, which hydrolyse cellulose and 40 hemicellulose; actin and other major components of the cytoskeleton. The DEGs were enriched 41 in antigen processing and presentation, carbon fixation in photosynthetic organisms, 42 glycolysis/gluconeogenesis, the MAPK signalling pathway, protein processing in the 43 endoplasmic reticulum and RNA degradation. Inoculation with F. mosseae could promote the 44 growth and development of soybean and improve disease resistance. This study provides an 45experimental basis for further research on the molecular mechanism underlying the alleviation 46 of challenges associated with the continuous cropping of soybean by AMF.
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