Mycorrhizal colonization of transgenic Eucalyptus camaldulensis carrying the mangrin gene for salt tolerance

Lelmen, Kipkorir E.; Yu, Xiang; Kikuchi, Akira; Shimazaki, Takayoshi; Mimura, M.; Watanabe, Kazuo

doi:10.5511/plantbiotechnology.27.339

Cited by 9 publications

(4 citation statements)

References 37 publications

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“…The EC at the surface (up to 5 cm deep) was >12 but was <4 in layers deeper than 20 cm ( Figure 2B). Salt tolerance of young trees was evaluated by treating the soil with a 200 mM NaCl solution (EC ∼11) (Lelmen et al 2010). The EC values at depths above 20 cm varied widely at the different points examined.…”

Section: Resultsmentioning

confidence: 99%

A field trial to assess the environmental biosafety of codA-transgenic Eucalyptus camaldulensis cultivation

Yu¹,

Kikuchi²,

Matsunaga³

et al. 2013

Plant Biotechnology

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The first Japanese field trial testing the effects of genetically modified (GM) forest trees was conducted over a period of 4 years. Salt tolerance was conferred on Eucalyptus camaldulensis by introducing the soil microbe-derived choline oxidase (codA) gene into this plant. Three individual lines were planted in an isolated field owned by the Gene Research Center at the University of Tsukuba, following approval for type I use by the Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Japanese Ministry of the Environment. Soil populations of fungi, actinomycetes, and bacteria fluctuated during sampling, but any differences introduced were observed only transiently, and no long-term change in the population was observed during the trial. Both the soil-mix as well as the sandwich method was employed to evaluate allelopathic activity of codA-transgenic E. camaldulensis. By the soil mix method, both GM and non GM-derived plant samples were found to exert negative allelopathic effects on seed germination, with an annual fluctuation. Overall, no significant differences were observed between the transformant lines and their bulk seed-derived non-transformants. The sandwich method also revealed an annual fluctuation. A comparison of the non-transformants and transformants revealed no significant differences in their allelopathic activity. Considering the practical use of these transgenic E. camaldulensis, they will no doubt be planted in some salinized sites. Thus, evaluating their impact on the ecosystem in saline conditioned fields might be important in the future.

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Section: Resultsmentioning

confidence: 99%

A field trial to assess the environmental biosafety of codA-transgenic Eucalyptus camaldulensis cultivation

Yu¹,

Kikuchi²,

Matsunaga³

et al. 2013

Plant Biotechnology

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“…These results corroborate other studies examining unpredicted, unintended effects of transgenic plants. Although such studies are rare when compared to those of predicted, unintended effects (e.g., effects of Btexpressing plants on nontarget insects, e.g., Axelsson et al, 2011), several have found no significant difference or detected only minor or temporary changes (Dunfield & Germida, 2003;Seppänen et al, 2007;Oliver et al, 2008;Lelmen et al, 2010;Vogler et al, 2010;Cheeke et al, 2015;Ricroch et al, 2018; see also transgenic chestnut nontarget studies mentioned in the Introduction). However, when significant effects have been found, they have often been restricted to a subset of hypotheses tested, vary among transgenic lines tested, or have magnitudes of effects within the range of untransformed isogenic plants (e.g., Brodeur-Campbell et al, 2006;Hjältén et al, 2007;Post & Parry, 2011;Turrini et al, 2015;Chen et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Comparative efficacy of gypsy moth (Lepidoptera: Erebidae) entomopathogens on transgenic blight‐tolerant and wild‐type American, Chinese, and hybrid chestnuts (Fagales: Fagaceae)

et al. 2019

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American chestnut (Castanea dentata [Marsh.] Borkh.) was once the dominant hardwood species in Eastern North America before an exotic fungal pathogen, Cryphonectria parasitica (Murrill) Barr, functionally eliminated it across its range. One promising approach toward restoring American chestnut to natural forests is development of blight‐tolerant trees using genetic transformation. However, transformation and related processes can result in unexpected and unintended phenotypic changes, potentially altering ecological interactions. To assess unintended tritrophic impacts of transgenic American chestnut on plant–herbivore interactions, gypsy moth (Lymantria dispar L.) caterpillars were fed leaf disks excised from two transgenic events, Darling 54 and Darling 58, and four control American chestnut lines. Leaf disks were previously treated with an LD50 dose of either the species‐specific Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) or the generalist pathogen Bacillus thuringiensis subsp. kurstaki (Btk). Mortality was quantified and compared to water blank controls. Tree genotype had a strong effect on the efficacies of both pathogens. Larval mortality from Btk‐treated foliage from only one transgenic event, Darling 54, differed from its isogenic progenitor, Ellis 1, but was similar to an unrelated wild‐type American chestnut control. LdMNPV efficacy was unaffected by genetic transformation. Results suggest that although genetic modification of trees may affect interactions with other nontarget organisms, this may be due to insertion effects, and variation among different genotypes (whether transgenic or wild‐type) imparts a greater change in response than transgene presence.

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“…It was reported that the ectopic expression of Mangrin in bacteria, yeast, and tobacco suspension‐cultured cells improved these organisms’ salt tolerance (Yamada et al ., ). We have also reported the development and evaluation of transgenic E. camaldulensis harbouring Mangrin , and we evaluated the plants’ salt tolerance in contained culture room cultivations and semi‐confined screen‐house trials (Lelmen et al ., ; Yu et al ., 2013d).…”

Section: Introductionmentioning

confidence: 98%

Development and evaluation of novel salt‐tolerantEucalyptustrees by molecular breeding using anRNA‐Binding‐Proteingene derived from common ice plant (Mesembryanthemum crystallinumL.)

Tran

Oguchi

Akatsuka

et al. 2018

Plant Biotechnology Journal

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The breeding of plantation forestry trees for the possible afforestation of marginal land would be one approach to addressing global warming issues. Here, we developed novel transgenic Eucalyptus trees (Eucalyptus camaldulensis Dehnh.) harbouring an RNA-Binding-Protein (McRBP) gene derived from a halophyte plant, common ice plant (Mesembryanthemum crystallinum L.). We conducted screened-house trials of the transgenic Eucalyptus using two different stringency salinity stress conditions to evaluate the plants' acute and chronic salt stress tolerances. Treatment with 400 mM NaCl, as the high-stringency salinity stress, resulted in soil electrical conductivity (EC) levels >20 mS/cm within 4 weeks. With the 400 mM NaCl treatment, >70% of the transgenic plants were intact, whereas >40% of the non-transgenic plants were withered. Treatment with 70 mM NaCl, as the moderate-stringency salinity stress, resulted in soil EC levels of approx. 9 mS/cm after 2 months, and these salinity levels were maintained for the next 4 months. All plants regardless of transgenic or non-transgenic status survived the 70 mM NaCl treatment, but after 6-month treatment the transgenic plants showed significantly higher growth and quantum yield of photosynthesis levels compared to the non-transgenic plants. In addition, the salt accumulation in the leaves of the transgenic plants was 30% lower than that of non-transgenic plants after 15-week moderate salt stress treatment. There results suggest that McRBP expression in the transgenic Eucalyptus enhances their salt tolerance both acutely and chronically.

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Mycorrhizal colonization of transgenic Eucalyptus camaldulensis carrying the mangrin gene for salt tolerance

Cited by 9 publications

References 37 publications

A field trial to assess the environmental biosafety of codA-transgenic Eucalyptus camaldulensis cultivation

A field trial to assess the environmental biosafety of codA-transgenic Eucalyptus camaldulensis cultivation

Comparative efficacy of gypsy moth (Lepidoptera: Erebidae) entomopathogens on transgenic blight‐tolerant and wild‐type American, Chinese, and hybrid chestnuts (Fagales: Fagaceae)

Development and evaluation of novel salt‐tolerantEucalyptustrees by molecular breeding using anRNA‐Binding‐Proteingene derived from common ice plant (Mesembryanthemum crystallinumL.)

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