Mycorrhiza Helper Bacteria

Tarkka, Mika; Frey‐Klett, Pascale

doi:10.1007/978-3-540-78826-3_6

Cited by 28 publications

(17 citation statements)

References 94 publications

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“…Dominant bacterial taxa found in this study, belonging to genera Sphingomonas , Pseudomonas , Massilia, and Methylobacterium, were previously identified in hydrocarbon‐polluted soils and were shown to be involved in biodegradation of PAHs (FDennis & Zylstra, ; Van Aken et al ., ; Zhou et al ., ; Ní Chadhain & Zylstra, ; Zhang et al ., ). Interestingly, the nondominant taxa found here, which belong to genera Bosea, Brevundimonas, Bradyrhizobium, and Paenibacillus, have been reported elsewhere to improve mycorrhizal colonization of roots and plant nutrient uptake (Frey‐Klett et al ., ; Tarkka & Frey‐Klett, ; Bonfante & Anca, ). The nondominant taxa we have found have also been considered to be members of a group called mycorrhiza helper bacteria (MHB), which includes phosphate‐solubilizing bacteria (PSB) and nitrogen‐fixing bacteria (Garbaye, ; Marschner & Timonen, ).…”

Section: Resultsmentioning

confidence: 99%

Bacteriaassociated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons

Iffis

St‐Arnaud

Hijri

2014

FEMS Microbiol Lett

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Arbuscular mycorrhizal fungi (AMF) belong to phylum Glomeromycota, an early divergent fungal lineage forming symbiosis with plant roots. Many reports have documented that bacteria are intimately associated with AMF mycelia in the soil. However, the role of these bacteria remains unclear and their diversity within intraradical AMF structures has yet to be explored. We aim to assess the bacterial communities associated within intraradical propagules (vesicles and intraradical spores) harvested from roots of plant growing in the sediments of an extremely petroleum hydrocarbon-polluted basin. Solidago rugosa roots were sampled, surface-sterilized, and microdissected. Eleven propagules were randomly collected and individually subjected to whole-genome amplification, followed by PCRs, cloning, and sequencing targeting fungal and bacterial rDNA. Ribotyping of the 11 propagules showed that at least five different AMF OTUs could be present in S. rugosa roots, while 16S rRNA ribotyping of six of the 11 different propagules showed a surprisingly high bacterial richness associated with the AMF within plant roots. Most dominant bacterial OTUs belonged to Sphingomonas sp., Pseudomonas sp., Massilia sp., and Methylobacterium sp. This study provides the first evidence of the bacterial diversity associated with AMF propagules within the roots of plants growing in extremely petroleum hydrocarbon-polluted conditions.

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Section: Resultsmentioning

confidence: 99%

Bacteriaassociated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons

Iffis

St‐Arnaud

Hijri

2014

FEMS Microbiol Lett

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“…Some studies have concentrated on the ‘helper’ role of bacteria in facilitating mycorrhizal colonization of roots in forest nursery environments (Garbaye, ; Frey‐Klett et al ., ; Labbé et al ., ). Stimulated germination of fungal propagules, promotion of mycelial growth/branching, reduced soil‐mediated stress, modification of root system architecture and effects on host recognition or receptivity have all been identified as possible mechanisms underlying the positive effects of the bacteria (Tarkka and Frey‐Klett, ; Aspray et al ., ). Other studies suggest that bacteria–mycorrhiza interactions may play a role in associative N‐fixation (Perez‐Moreno and Read, ; Paul et al ., ), and the presence and expression of bacterial nifH nitrogenase genes has been shown by Izumi et al .…”

Section: Introductionmentioning

confidence: 99%

Analysis of single root tip microbiomes suggests that distinctive bacterial communities are selected by Pinus sylvestris roots colonized by different ectomycorrhizal fungi

Marupakula

Mahmood

Finlay

2015

Environmental Microbiology

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Symbiotic ectomycorrhizal tree roots represent an important niche for interaction with bacteria since the fungi colonizing them have a large surface area and receive a direct supply of photosynthetically derived carbon. We examined individual root tips of Pinus sylvestris at defined time points between 5 days and 24 weeks, identified the dominant fungi colonizing each root tip using Sanger sequencing and the bacterial communities colonizing individual root tips by 454 pyrosequencing. Bacterial colonization was extremely dynamic with statistically significant variation in time and increasing species richness until week 16 (3477 operational taxonomic units). Bacterial community structure of roots colonized by Russula sp. 6 GJ-2013b, Piloderma spp., Meliniomyces variabilis and Paxillus involutus differed significantly at weeks 8 and 16 but diversity declined and significant differences were no longer apparent at week 24. The most common genera were Burkholderia, Sphingopyxsis, Dyella, Pseudomonas, Acinetobacter, Actinospica, Aquaspirillum, Acidobacter Gp1, Sphingomonas, Terriglobus, Enhydrobacter, Herbaspirillum and Bradyrhizobium. Many genera had high initial abundance at week 8, declining with time but Dyella and Terriglobus increased in abundance at later time points. In roots colonized by Piloderma spp. several other bacterial genera, such as Actinospica, Bradyrhizobium, Acidobacter Gp1 and Rhizomicrobium appeared to increase in abundance at later sampling points.

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“…It is estimated that 90-99% soil microorganisms belong to the VBNC [9]. On the one hand, it may mean that cultured MHB represent only a small fraction of all bacteria from the rhizosphere, on the other hand, we can say that the MHB represent the dominant bacterial group capable of being cultivated among groups characterized to date [21].…”

Section: Methodsmentioning

confidence: 99%

The Application of PCR Reaction for Identification of MHB Bacteria Species

Ząbkiewicz¹,

Myga-Nowak²,

Bandurska³

et al. 2014

Archives of Environmental Protection

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This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplifi ed samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fl uorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.

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Mycorrhiza Helper Bacteria

Cited by 28 publications

References 94 publications

Bacteriaassociated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons

Bacteriaassociated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons

Analysis of single root tip microbiomes suggests that distinctive bacterial communities are selected by Pinus sylvestris roots colonized by different ectomycorrhizal fungi

The Application of PCR Reaction for Identification of MHB Bacteria Species

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