Mycoremediation of vinasse by surface response methodology and preliminary studies in air-lift bioreactors

Aragão, Moniky S.; Menezes, Diego Batista; Ramos, Larissa Castor; Oliveira, Helon Simôes; Bharagava, Ram Naresh; Ferreira, Luiz Fernando Romanholo; Teixeira, J. A.; Ruzene, Denise Santos; Silva, Daniel P.

doi:10.1016/j.chemosphere.2019.125432

Cited by 25 publications

(8 citation statements)

References 39 publications

(52 reference statements)

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“…In this study, a new type of oxygen-enhanced fermentation equipment was designed to maximize the efficiency of the oxygen supply to the equipment. The equipment combines the advantages of mechanically stirred and air-lift bioreactors ( Aragão et al, 2020 ). We installed a liquid flow guide inside the conventional bioreactor, which solves the problem of the chaotic flow of the fermentation broth inside the tank, and tends to form dead ends for agitation ( Farkya et al, 2004 ; Gill et al, 2008 ).…”

Section: Resultsmentioning

confidence: 99%

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Wang

Zhao

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: L-lysine is a bulk product. In industrial production using high-biomass fermentation, the high density of bacteria and the intensity of production require sufficient cellular respiratory metabolism for support. Conventional bioreactors often have difficulty meeting the oxygen supply conditions for this fermentation process, which is not conducive to improving the sugar-amino acid conversion rate. In this study, we designed and developed an oxygen-enhanced bioreactor to address this problem.Methods: This bioreactor optimizes the aeration mix using an internal liquid flow guide and multiple propellers.Results: Compared with a conventional bioreactor, it improved the kLa from 367.57 to 875.64 h-1, an increase of 238.22%. The results show that the oxygen supply capacity of the oxygen-enhanced bioreactor is better than that of the conventional bioreactor. Its oxygenating effect increased the dissolved oxygen in the middle and late stages of fermentation by an average of 20%. The increased viability of Corynebacterium glutamicum LS260 in the mid to late stages of growth resulted in a yield of 185.3 g/L of L-lysine, 74.57% conversion of lysine from glucose, and productivity of 2.57 g/L/h, an increase of 11.0%, 6.01%, and 8.2%, respectively, over a conventional bioreactor. Oxygen vectors can further improve the production performance of lysine strains by increasing the oxygen uptake capacity of microorganisms. We compared the effects of different oxygen vectors on the production of L-lysine from LS260 fermentation and concluded that n-dodecane was the most suitable. Bacterial growth was smoother under these conditions, with a 2.78% increase in bacterial volume, a 6.53% increase in lysine production, and a 5.83% increase in conversion. The different addition times of the oxygen vectors also affected the final yield and conversion, with the addition of oxygen vectors at 0 h, 8 h, 16 h, and 24 h of fermentation increasing the yield by 6.31%, 12.44%, 9.93%, and 7.39%, respectively, compared to fermentation without the addition of oxygen vectors. The conversion rates increased by 5.83%, 8.73%, 7.13%, and 6.13%, respectively. The best results were achieved by adding oxygen vehicles at the 8th hour of fermentation, with a lysine yield of 208.36 g/L and a conversion rate of 83.3%. In addition, n-dodecane significantly reduced the amount of foam produced during fermentation, which is beneficial for fermentation control and equipment.Conclusion: The new oxygen-enhanced bioreactor improves oxygen transfer efficiency, and oxygen vectors enhance the ability of cells to take up oxygen, which effectively solves the problem of insufficient oxygen supply during lysine fermentation. This study provides a new bioreactor and production solution for lysine fermentation.

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Section: Resultsmentioning

confidence: 99%

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Wang

Zhao

et al. 2023

Front. Bioeng. Biotechnol.

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“…Despite all, the legal framework regarding the adequate use of vinasse in Brazil with regard to São Paulo Decree Law Number 8.468, September 8, 1976, it can be, considered superficial because it's prescribed only according to its potassium content. However, its potential associated environmental impacts weren't considered (Moares et al, 2015 andAragao, et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Vinasse: current concepts, challenges and opportunities for the sustainability

Araujo¹,

Niño-Castillo

2021

EJRN

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The objective was to highlight the historical documents that foresee the use and abuse of ethanol production (vinasse) in Brazil according to literature published in the period 1934-2020. The methodology used was documentary, the review and analysis of which brought with it the synthesis and perspectives of vinasse as well as its uses and abuses. Results: a) there are legal instruments in Brzil since 1934 whose goal is to care for the environment; b) about in 1978 was prohibited the direct dumping of stillage into bodies of water and c) in 2011 environmental guidelines were provided on this topic to achieve sustainability in the Brazilian space. Conclusions: 1) as can be seen in the cited literature, vinasse is one of the most impressive respurces for ethanol production; 2) Brazil is the second largest ethanol producer worldwide; 3) the use of stillage for cooling sugarcane fiekds, its organic content, chemical and biochemical oxygen demand rates are high and 4) the synergy between academics from universities, the government sector, civil society, companies in the productive chain of the sugar energy sector, among others, is important.

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“…As an alternative, it can be used in bioreactors. Bioreactors are systems that have their physicochemical properties constantly maintained to promote microbial growth (Aragão et al, 2020). Various harmful compounds can be degraded by fungi.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of Heavy Metals by using Aspergillus niger and Candida albicans

2023

ZJPAS

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Bioremediation is a branch of biotechnology that employs the use of living organisms, like microalgae and fungi, in the removal of contaminants, pollutants, and toxins from soil, water, and other environments. The study was design to know and evaluate the efficiency of fungi to remediate two types of heavy metals (Pb and Cd), by using different concentrations (5, 15, 35, and 50ppm). Results revealed that the lowest applied dose (5ppm) of both tested heavy metals had the highest reduction percent by using two fungal stains Aspergillus niger and Candida albicans were remove lead by 85.6 and 84.2%, while for cadmium were 80 and 78.4% respectively. Statistically significant differences (P≤0.05) were observed between control and the treatments for both tested heavy metals. Highest heavy metals removal was found at the end of experiment (20 days).

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Mycoremediation of vinasse by surface response methodology and preliminary studies in air-lift bioreactors

Cited by 25 publications

References 39 publications

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Vinasse: current concepts, challenges and opportunities for the sustainability

Bioremediation of Heavy Metals by using Aspergillus niger and Candida albicans

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