Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri

Manso-Silván, Lucía; Vilei, Edy M.; Sachse, Konrad; Djordjevic, Steven P.; Thiaucourt, François; Frey, Joachim

doi:10.1099/ijs.0.005546-0

Cited by 129 publications

(78 citation statements)

References 43 publications

(58 reference statements)

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“…mycoides (Mmm) (Manso-Silván et al 2009;Tardy et al 2011). It is characterized by sero-fibrinous interlobular oedema and hepatization giving a marbled appearance to the lung in acute and sub-acute cases and capsulated lesions (sequestra) in the lungs of chronically infected cattle (Radostits et al 2007;Schnee et al 2011;Tardy et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Using sero-positivity to assess geospatial burden of contagious bovine pleuropneumonia on pastoral cattle herds of north-central Nigeria

2016

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Contagious bovine pleuropneumonia (CBPP) is an infectious disease of cattle endemic in sub-Saharan Africa. A cross-sectional sero-geospatial survey was conducted to assess the CBPP sero-geospatial burdens among estimate at-risk sero-positive cattle in nomadic and sedentary pastoral cattle production systems of north-central Nigeria, between January and August 2013. A total of 765 cattle in 125 nomadic herds and 375 cattle in 125 sedentary herds were sampled. Sera were analysed using c-ELISA. OpenEpi version 2.3 was used for statistical analyses. Geo-coordinates of herds were taken using Garmin GPS, while ArcGIS 9.3 was used to map geospatial data of sero-positive cattle and herds in the agro-geographical zones. In nomadic production, 16.2 % (95 % CI 13.7 to 19.0.) cattle were sero-positive in 47.2 % (95 % CI 38.2 to 56.3) herds. And the sedentary system had 9.6 % (95 % CI 6.9 to 12.0) sero-positive cattle in 27.2 % (95 % CI 19.6 to 35.9) herds. Agro-geographical zone A was more likely (OR 3.42; 95 % CI 1.90, 6.15) to have significant impacts on cattle-level sero-geospatial burden than Agro-geographical zone B. Also, Agro-geographical zone C was more likely (OR 5.14; 95 % CI 2.91, 9.08) to have significant impacts on cattle-level sero-geospatial burden than Agro-geographical zone B. The developed GIS CBPP risk maps showed various densities of its burdens in the agro-zones. The visualized proportional circle maps presented GIS usefulness in the active surveillance of CBPP, and if used in conjunction with sero-diagnosis, the maps would aid policymakers with practical imageries for livestock disease control decisions in pastoral cattle herds.

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Section: Introductionmentioning

confidence: 99%

Using sero-positivity to assess geospatial burden of contagious bovine pleuropneumonia on pastoral cattle herds of north-central Nigeria

2016

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“…Mycoplasma strains transformed were M. genitalium strain MS5 (21), M. mycoides subspecies capri strain GM12 (22,23) and M. pneumoniae strain M129-B170 (ATCC 29343). All were cultured in SP-4 medium (24) at 37 C and 5% carbon dioxide (CO 2 ).…”

Section: Mycoplasma Strains and Culture Conditionsmentioning

confidence: 99%

Cloning Whole Bacterial Genomes in Yeast

Benders

2012

Methods in Molecular Biology

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Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.

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“…Mmc and Mcc are reported to cause a pattern of disease called contagious agalactia syndrome (CA) including mastitis, arthritis, pulmonary diseases, and septicaemia especially in goats (25,26,29). All three caprine pathogens can be identified and distinguished by both PCR and biochemical characterisation of their different nucleotide sequences and biochemical activity (14,15,19).…”

Section: Introductionmentioning

confidence: 99%

“…When they were regarded as two subspecies, numerical analysis of one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) protein patterns (6,16), two-dimensional PAGE protein patterns (21,22), and serological analysis (20) showed them to be very similar antigenically. They have consequently now been grouped into a single subspecies (19,28).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Wang

Yang

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley's medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

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Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri

Cited by 129 publications

References 43 publications

Using sero-positivity to assess geospatial burden of contagious bovine pleuropneumonia on pastoral cattle herds of north-central Nigeria

Using sero-positivity to assess geospatial burden of contagious bovine pleuropneumonia on pastoral cattle herds of north-central Nigeria

Cloning Whole Bacterial Genomes in Yeast

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

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