Mycoplasma gallisepticum strain ts-304 is a safe and effective live attenuated vaccine for use in chickens

Condello, Anna Kanci; Underwood, Gregory J.; Shil, Pollob; Noormohammadi, Amir H.; Markham, Philip F.; Wawegama, Nadeeka K.; Browning, Glenn F.

doi:10.1016/j.vetmic.2020.108654

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…Another fifteen control birds were obtained from the SPF flock close to the time of experimental challenge and divided into two groups, a challenged-only positive control group (Group 3), containing 10 birds, and an uninfected negative control group (Group 4), containing 5 birds. Fifty-seven weeks after vaccination, birds from Groups 1 (vaccinated-and-challenged) and 3 (challengedonly) were exposed to an aerosol of the M. gallisepticum wildtype strain Ap3AS using an established protocol (22,30). The birds in Groups 3 and 4 were 70 weeks of age at the time of experimental challenge.…”

Section: Experimental Vaccination and Infection Of Chickensmentioning

confidence: 99%

“…These characteristics of the immunity induced by vaccination with ts-11 are likely to be due to the presence of two variants in the commercial Vaxsafe MG (ts-11) vaccine, one with an intact gapA gene (GapA + ) and a second with a 20 bp repeat in the gapA gene (GapA -) that induces a frameshift and results in premature termination of translation of the gene (20). A GapA + clone, strain ts-304, has been isolated from ts-11 and has been shown to be as safe in chickens as the ts-11 strain from which it was derived, and as effective, but at a lower dose (21,22). The ts-304 vaccine has also been shown to colonize the trachea of turkeys and protect against experimental challenge with the M. gallisepticum wild-type strain Ap3AS (23,24).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

et al. 2021

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Live attenuated vaccines are commonly used to control Mycoplasma gallisepticum infections in chickens. M. gallisepticum ts-304 is a novel live attenuated vaccine strain that has been shown to be safe and effective. In this study, the transcriptional profiles of genes in the tracheal mucosa in chickens challenged with the M. gallisepticum wild-type strain Ap3AS at 57 weeks after vaccination with ts-304 were explored and compared with the profiles of unvaccinated chickens that had been challenged with strain Ap3AS, unvaccinated and unchallenged chickens, and vaccinated but unchallenged chickens. At two weeks after challenge, pair-wise comparisons of transcription in vaccinated-only, vaccinated-and-challenged and unvaccinated and unchallenged birds detected no differences. However, the challenged-only birds had significant up-regulation in the transcription of genes and enrichment of gene ontologies, pathways and protein classes involved in infiltration and proliferation of inflammatory cells and immune responses mediated through enhanced cytokine and chemokine production and signaling, while those predicted to be involved in formation and motor movement of cilia and formation of the cellular cytoskeleton were significantly down-regulated. The transcriptional changes associated with the inflammatory response were less severe in these mature birds than in the relatively young birds examined in a previous study. The findings of this study demonstrated that vaccination with the attenuated M. gallisepticum strain ts-304 protects against the transcriptional changes associated with the inflammatory response and pathological changes in the tracheal mucosa caused by infection with M. gallisepticum in chickens for at least 57 weeks after vaccination.

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Section: Experimental Vaccination and Infection Of Chickensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

et al. 2021

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“…The commercially available live attenuated M. gallisepticum vaccines used for immunization of chickens include F strain (22), strain 6/85 (25), and strain ts-11 (26). A new vaccine candidate, MG ts-304 (27), has been shown to have a safety profile similar to that of ts-11 but to be efficacious at a lower dose (97). The transcriptional response in the tracheal mucosa has been investigated (17) after exposure to M. gallisepticum vaccine strains GT5 and Mg7 (16) over the first 2 days after exposure, but there have been no previous studies investigating the transcriptional profile in the trachea after challenge with wild-type M. gallisepticum in vaccinated birds.…”

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confidence: 99%

Differential Response of the Chicken Trachea to Chronic Infection with Virulent Mycoplasma gallisepticum Strain Ap3AS and Vaxsafe MG (Strain ts-304): a Transcriptional Profile

et al. 2020

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Mycoplasma gallisepticum is the primary etiological agent of chronic respiratory disease in chickens. Live attenuated vaccines are most commonly used in the field to control the disease, but current vaccines have some limitations. Vaxsafe MG (strain ts-304) is a new vaccine candidate that is efficacious at a lower dose than the current commercial vaccine strain ts-11, from which it is derived. In this study, the transcriptional profiles of the trachea of unvaccinated chickens and chickens vaccinated with strain ts-304 were compared 2 weeks after challenge with M. gallisepticum strain Ap3AS during the chronic stage of infection. After challenge, genes, gene ontologies, pathways, and protein classes involved in inflammation, cytokine production and signaling, and cell proliferation were upregulated, while those involved in formation and motor movement of cilia, formation of intercellular junctional complexes, and formation of the cytoskeleton were downregulated in the unvaccinated birds compared to the vaccinated birds, reflecting immune dysregulation and the pathological changes induced in the trachea by infection with M. gallisepticum. Vaccination appears to protect the structural and functional integrity of the tracheal mucosa 2 weeks after infection with M. gallisepticum.

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“…Despite the rapid growth of the industry, one of the major constraints of poultry farming in Bangladesh is the outbreaks of different infectious diseases (3). Among the major health hazards caused by different microbial agents being faced by poultry birds, mycoplasmosis is of great importance which brings serious monetary damage for poultry industry (4)(5)(6). Mycoplasmosis is a chronic respiratory disease of poultry (7) caused by Mycoplasma gallisepticum and Mycoplasma synovia (8,9) and less commonly by Mycoplasma meleagridis and Mycoplasma iowae (10).…”

Section: Introductionmentioning

confidence: 99%

“…Control of MG infection in poultry farms can be achieved by fetching birds from MG infection free sources and employing vaccination program (5,22,23). Mycoplasmosis has been a huge burden for the poultry industry of Bangladesh for many years because it costing Bangladesh a huge economic loss by alleviating feed conversion ratio, weight gain and by diminishing egg production and egg hatchability in layers.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Mycoplasma gallisepticum infection in layer chickens of Bangladesh

Raquib¹,

Uddin²,

Nurozzaman³

et al. 2021

IJVS

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Mycoplasma gallisepticum causes major health hazards in poultry birds in Bangladesh which results in huge economic losses every year. This study was carried out to estimate and analyze the prevalence of Mycoplasma gallisepticum (MG) infection in commercial layer chickens at Kishoreganj district of Bangladesh during the period from November 2018 to October 2019. A total of 505 serum samples from 94 commercial layer farms of Kishoreganj Sadar Upazila and Pakundia Upazila of Kishoreganj district were collected. Serum plate agglutination (SPA) was performed to detect the antibody against MG. Prevalence was found 73% in the Kishoreganj district by SPA test. MG was significantly (P<0.01) more prevalent in Pakundia Upazila 82% than Kishoreganj Sadar Upazila 61.11%. In case of season, winter season had significantly higher (X^2=30.94, p=0.000) prevalence of MG infection. In relation to age, seroprevalence of MG infection was highest 78% in birds of 65 weeks' age and lowest 71% in 6-25 weeks' age birds. Any significant (P>0.05) association was not found between flock size and seroprevalence of MG. Seroprevalence was highest in flock containing above 2600 birds. MG infection is prevalent in the chicken population of Kishoreganj district, Bangladesh. Measures should be taken for successful prevention and control of this disease in Bangladesh.

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Mycoplasma gallisepticum strain ts-304 is a safe and effective live attenuated vaccine for use in chickens

Cited by 15 publications

References 23 publications

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

Differential Response of the Chicken Trachea to Chronic Infection with Virulent Mycoplasma gallisepticum Strain Ap3AS and Vaxsafe MG (Strain ts-304): a Transcriptional Profile

Seroprevalence of Mycoplasma gallisepticum infection in layer chickens of Bangladesh

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