Mycogenesis of gold nanoparticles using a phytopathogen Alternaria alternata

Abstract

The development of an eco-friendly and reliable process for the synthesis of gold nanomaterials (AuNPs) using microorganisms is gaining importance in the field of nanotechnology. In the present study, AuNPs have been synthesized by bio-reduction of chloroauric acid (HAuCl(4)) using the fungal culture filtrate (FCF) of Alternaria alternata. The synthesis of the AuNPs was monitored by UV-visible spectroscopy. The particles thereby obtained were characterized by UV, dynamic light scattering (DLS), X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). Energy-dispersive X-ray study revealed the presence of gold in the nanoparticles. Fourier transform infrared spectroscopy confirmed the presence of a protein shell outside the nanoparticles which in turn also support their stabilization. Treatment of the fungal culture filtrate with aqueous Au(+) ions produced AuNPs with an average particle size of 12 ± 5 nm. This proposed mechanistic principal might serve as a set of design rule for the synthesis of nanostructures with desired architecture and can be amenable for the large scale commercial production and technical applications.

Green synthesis of gold nanoparticles from Scutellaria barbata and its anticancer activity in pancreatic cancer cell (PANC‐1)

“…Among these sources, plants are commonly known as the "factories of bio-nanoparticles". Moreover, they have been considered as being eco-friendly, reasonably cheap to produce, having a unique structure and having the highest capacity of uptake of metallic ions [26][27][28].…”

Extracellular mycosynthesis of gold nanoparticles using Trichoderma hamatum : optimization, characterization and antimicrobial activity

“…The sharp bands at 3450 and 2929 cm −1 suggest the presence of stretching vibrations (N–H) of primary and secondary amines, and aldehydic C‐H stretching respectively (Sarkar et al . ). Moreover, this also indicates the possibility of hydroxyl functional groups, which might have arisen from alcohols or phenols present in the fungal filtrate.…”

Mycofabrication of common plasmonic colloids, theoretical considerations, mechanism and potential applications

“…In a similar study the phytopathogen Alternaria alternata produced a mixture of AuNPs. Smaller spherical particles of the size between 8nm and 20nm, and large nanotriangles of 30 nm-100 nm [112].…”