Mycogenesis of cerium oxide nanoparticles using Aspergillus niger culture filtrate and their applications for antibacterial and larvicidal activities

Abstract

Cerium oxide nanoparticles (CeO 2 NPs) were synthesized using Aspergillus niger culture filtrate. The mycosynthesized CeO 2 NPs were characterized by UVVisible (UV-Vis), Fourier Transform Infrared (FT-IR), X-ray diffraction (XRD), Micro Raman, Thermogravimetric/Differential Thermal Analysis (TG/DTA), Photoluminescence, and Transmission Electron Microscopy (TEM) analyses. UV-Vis spectrum exhibited a corresponding absorption peak for CeO 2 NPs at 296 nm, and the functional groups present in the fungal filtrate responsible for the synthesis of NPs were analyzed by FT-IR. The further characterization of the mycosynthesized CeO 2 NPs revealed particles of the cubic structure and spherical shape, with the particle sizes ranging from 5 to 20 nm. The antibacterial activity of CeO 2 NPs was examined in respect of two Gram-positive (G?) bacteria (Streptococcus pneumoniae, Bacillus subtilis) and two Gram-negative (G-) bacteria (Proteus vulgaris, Escherichia coli) by disk diffusion method. The test results for CeO 2 NPs at a concentration of 10 mg/mL showed higher activities on the zone of inhibition of up to 10.67 ± 0.33 and 10.33 ± 0.33 mm against Streptococcus pneumonia and Bacillus subtilis, respectively, The CeO 2 NPs caused 100 % mortality on first instar of Aedes aegypti at 0.250 mg/L concentration after 24-h exposure. The mycosynthesis of CeO 2 NPs is a simple, cost-effective and eco-friendly approach and it will also potentially helpful to control pathogenic bacteria and dengue vector.

Skirmishing MDR strain of Candida albicans by effective antifungal CeO2 nanostructures using Aspergillus terreus and Talaromyces purpurogenus

“…No extra peak is observed that confirms the purity of sample. These results are concordant to the previous literature [12,18].…”

“…CeO 2 nanostructures by Aspergillu sterreus had the highest intensity peaks seen at 2θ=28.49°with (111) plane which is the lattice plane and other peaks were at 33.01°, 47.42°, 56.28°, 59.01°, 69.34°, 76.66°and 79.17°indexed at 200, 220, 311, 222, 400, 331 and 420 planes, respectively. Plane values were closely agreed with JCPDS Card # 89-8436 standard diffraction data [12,18]. The sizes of particles revealed by XRD were 28.5 nm.…”

“…There are many reports on transition metal nanoparticles. Among them, some recent reports are on the synthesis of CeO 2 NPs using Humicola sp and Aspergillus niger [17,18].…”

Influence of Mg Doping on ZnO Nanoparticles for Enhanced Photocatalytic Evaluation and Antibacterial Analysis

“…Zinc oxide NPs have the ability to disrupt the gram-negative cell membrane structure of Escherichia coli [25, 26]. It was reported also that nanoparticles with a positive charge could bind the gram-negative cell membrane using electrostatic attraction [27]. ZnO NPs doped with different metal ions were evaluated against E. coli , and Staphylococcus aureus showed the antibacterial activity increasing with crystallite size [28].…”

Accelerated dephosphorylation of adenosine phosphates and related compounds in the presence of nanocrystalline cerium oxide

“…7,13 There have also been numerous attempts to apply cerium oxide in cancer therapy, [14][15][16][17] often in combination with chemotherapy or radiotherapy. 16,17 Cerium oxide has been tested as an antibacterial agent, 18 for the treatment of inflammatory disorders 19,20 and in anti-obesity formulations. 21 In nanocrystalline forms, cerium oxide is able to mimic the activities of some enzymes (superoxidedismutase, catalase, oxidase) -therefore, the term "nanozyme" was introduced for this type of biologically active nanomaterial.…”