Mycobacterium tuberculosis subsp. caprae subsp. nov.: A taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain

Aranaz, Alicia; Liébana, Ernesto; Gómez-Mampaso, E; Galán, Juan Carlos; Cousins, Debby; Ortega, A; Blázquez, Jesús; Baquero, Fernando; Mateos, Ana; Suárez, G.; Domı́nguez, Lucas

doi:10.1099/00207713-49-3-1263

Cited by 160 publications

(104 citation statements)

References 9 publications

(14 reference statements)

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“…Similar small differences in spoligotype were evident among a group of 'M. caprae' isolates (Aranaz et al, 1999). Spoligotyping has previously been used to define clonal relationships of the Beijing family of M. tuberculosis (van Soolingen et al, 1995; Qian Table 2.…”

Section: Dna Spoligotypingmentioning

confidence: 54%

“…More recently, two novel strains have been described: 'Mycobacterium canettii ', a novel smooth variant of M. tuberculosis that was first isolated from a Somali-born patient (van Soolingen et al, 1997) and subsequently from a Swiss patient exposed in Africa (Pfyffer et al, 1998); and 'Mycobacterium caprae' (basonym: M. tuberculosis subsp. caprae), a strain that occurs primarily in Spanish goats and has recently been elevated to species level (Aranaz et al, 1999;Niemann et al, 2002;Aranaz et al, 2003). M. tuberculosis, M. africanum, M. microti and M. bovis were accepted as separate species by using a combination of phenotypic characteristics and apparent host specificity.…”

Section: Introductionmentioning

confidence: 99%

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Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Cousins

Bastida

Cataldi

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

273

182

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A comparison of Mycobacterium tuberculosis complex isolates from seals (pinnipeds) in Australia, Argentina, Uruguay, Great Britain and New Zealand was undertaken to determine their relationships to each other and their taxonomic position within the complex. Isolates from 30 cases of tuberculosis in six species of pinniped and seven related isolates were compared to representative and standard strains of the M. tuberculosis complex. The seal isolates could be distinguished from other members of the M. tuberculosis complex, including the recently defined 'Mycobacterium canettii ' and 'Mycobacterium caprae', on the basis of host preference and phenotypic and genetic tests. Pinnipeds appear to be the natural host for this 'seal bacillus', although the organism is also pathogenic in guinea pigs, rabbits, humans, Brazilian tapir (Tapirus terrestris) and, possibly, cattle. Infection caused by the seal bacillus is predominantly associated with granulomatous lesions in the peripheral lymph nodes, lungs, pleura, spleen and peritoneum. Cases of disseminated disease have been found. As with other members of the M. tuberculosis complex, aerosols are the most likely route of transmission. The name Mycobacterium pinnipedii sp. nov. is proposed for this novel member of the M. tuberculosis complex (the type strain is 6482 T =ATCC BAA-688 T =NCTC 13288 T ).Abbreviations: BCG, Bacille Calmette-Gué rin; FAFLP, fluorescent amplified fragment length polymorphism; PZA, pyrazinamide; SS, seal spoligotype; TCH, thiophen-2-carboxylic acid hydrazide.

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Section: Dna Spoligotypingmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Cousins

Bastida

Cataldi

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

273

182

View full text Add to dashboard Cite

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“…Med. -Czech, 48, 2003(5): 113-125 1999Aranaz et al, 1999;Dvorska et al, 2001). None of the above mentioned species was isolated in other animals in this period, with the exception of one isolation of M. bovis in 1999 from one farm-reared deer (Pavlik et al, 2002b,c,d,e;Machackova et al, 2000Machackova et al, , 2003.…”

Section: Laboratory Examination Of the Tissues Samples Of Pigsmentioning

confidence: 99%

Tuberculous lesions in pigs in the Czech Republic in the years 1990-1999: occurrence, causal factors and economic losses

Pavlík¹,

Mátlová²,

Dvorská³

et al. 2003

Vet. Med. - Czech

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ABSTRACT:In the decade monitored a total of 45 873 318 pigs were slaughtered and examined according to veterinary hygiene standards. Apart from 1991, when results of tuberculous findings were not obtained, tuberculous lesions were found in 134 088 (0.32%) of the 41 458 565 pigs examined in the remaining nine years. During a detailed analysis of the pathological anatomical examination of 190 940 pigs slaughtered in one district, tuberculous lesions in lymph nodes were found in 4 107 (2.2%) pigs: mesenteric (65.3% pigs), submandibular (18.6% pigs), inguinal (0.1% pigs) and simultaneously intestinal and head lymph nodes (15.9% pigs). Miliary tuberculosis was found only in the parenchymatous organs of four (0.1%) pigs. The following financial losses resulted: 6% for confiscating the head, intestines and stomach, and from 22 to 24% for assessing meat as conditionally edible a�er processing, i.e. intended only for heat-processed products. Mycobacteria were isolated from 7 246 (41.8%) pigs through the cultivation of tissue samples from 17 326 pigs. Mycobacterium bovis was detected in only five (0.07%) animals which originated from the last outbreak of bovine tuberculosis in ca�le in the Czech Republic in 1995. M. avium complex (MAC) isolates came from 6 870 (94.8%) animals: 55.7% M. a. avium isolates were mainly of serotypes 2 and 3 and genotype IS901+ and IS1245+ and 39.2% M. a. hominissuis isolates were mainly of serotypes 4, 8 and 9 and genotype IS901-and IS1245+. Conditionally pathogenic mycobacteria (M. chelonae, M. terrae, M. phlei and M. fortuitum) were isolated from 371 (5.1%) pigs. In the whole period monitored, two marked increases in the findings of tuberculous lesions were recorded: In the mid-1990s as a result of using deep bedding with wood shavings and at the end of the 1990s as a result of supplementing the pigs' feed with peat. The predominant occurrence of M. a. avium isolates of genotype IS901+ and IS1245+ in the first half of the 1990s was replaced above all by M. a. hominissuis isolates of genotype IS901-and IS1245+. The reason for this was probably a change in the sources of infection for pigs. While at the beginning of the 1990s the most frequent source of infection were wild and domestic birds, various parts of the external environment became the source of the infection for pigs from the mid-1990s. In the years 1996 to 1999, Rhodococcus equi was isolated from 203 (11.6%) of the 1 745 animals examined. It was solely isolated from 154 (8.8%) animals and from 49 (2.8%) animals together with mycobacteria.

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“…More recently, three new members of the complex have been described: M. canetti [4], a smooth variant that was isolated first from a Somali patient; M. bovis subsp. caprae, related closely to 'classical' M. bovis, infecting primarily goats in Spain and humans and cattle in Central Europe [5,6], causing one-third of all human M. bovis cases of tuberculosis in Germany; and finally, M. pinnipedii, which causes tuberculosis in marine mammals [7][8][9]. Despite the high overall genetic relationship, the species of the MTBC show variability in their phenotypes, host range and importance for human TB.…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium boviswith different genotypes and from different hosts induce dissimilar immunopathological lesions in a mouse model of tuberculosis

León

Zumárraga

Oropeza

et al. 2009

Clinical and Experimental Immunology

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SummaryWith the hypothesis that genetic variability of Mycobacterium bovis could influence virulence and immunopathology, five M. bovis strains were selected from an epidemiological study in Argentina on the basis of their prevalence in cattle and occurrence in other species. We then determined the virulence and the immunopathology evoked by these strains in a well-characterized mouse model of progressive pulmonary tuberculosis. The reference strain AN5 was used as a control. BALB/c mice infected with this M. bovis reference strain showed 50% survival after 4 months of infection, with moderate bacillary counts in the lung. Two weeks after inoculation, it induced a strong inflammatory response with numerous granulomas and progressive pneumonia. In contrast, strain 04-303, isolated from a wild boar, was the most lethal and its most striking feature was sudden pneumonia with extensive necrosis. Strain 04-302, also isolated from wild boar but with a different spoligotype, induced similar pathology but to a lesser extent. In contrast, strains 534, V2 (both from cattle) and 02-2B (from human) were less virulent, permitting higher survival after 4 months of infection and limited tissue damage. Strain AN5 and the cattle and human isolates induced rapid, high and stable expression of interferon (IFN)-g and inducible nitric oxide synthase (iNOS). In contrast, the more virulent strains induced lower expression of IFN-g, tumour necrosis factor-a and iNOS. Interestingly, these more virulent strains induced very low expression of murine beta defensin 4 (mBD-4); whereas, the control strain AN5 induced progressive expression of this anti-microbial peptide, peaking at day 120. The less virulent strains induced high mBD-4 expression during early infection. Thus, as reported with clinical isolates of M. tuberculosis, M. bovis also showed variable virulence. This variability can be attributed to the induction of a different pattern of immune response.

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Mycobacterium tuberculosis subsp. caprae subsp. nov.: A taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain

Cited by 160 publications

References 9 publications

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Tuberculous lesions in pigs in the Czech Republic in the years 1990-1999: occurrence, causal factors and economic losses

Mycobacterium boviswith different genotypes and from different hosts induce dissimilar immunopathological lesions in a mouse model of tuberculosis

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