Mycobacterium tuberculosis strains modify granular enzyme secretion and apoptosis of human neutrophils

Hilda, J. Nancy; Meenakshi, N.; Das, Sulochana D.

doi:10.1016/j.molimm.2015.09.019

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…In humans, it is evident from our previous work that MTB strains influence neutrophil enzyme secretion. 35 In mice, serine protease activity has been shown to play a protective role within hypoxic regions of lung granulomas, the hallmark of MTB infection. 36 Granuloma was once believed to be an uniquely host-driven response, set to constrain MTB and prevent dissemination of bacilli.…”

Section: Neutrophil Enzymesmentioning

confidence: 99%

Role of neutrophils in tuberculosis: A bird's eye view

et al. 2019

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Neutrophils are innate immune cells implicated in the process of killing Mycobacterium tuberculosis early during infection. Once the mycobacteria enter the human system, neutrophils sense and engulf them. By secreting bactericidal enzymes and α-defensins like human neutrophil peptides loaded in their granule armory, neutrophils kill the pathogen. Peripheral blood neutrophils secrete a wide range of cytokines like IL-8, IL-1-β and IFN-γ in response to mycobacterial infection. Thus they signal and activate distant immune cells thereby informing them of prevailing infection. The activated monocytes, dendritic cells and T cells further continue the immune response. As a final call, neutrophils release neutrophil extracellular traps in circulation which can trap mycobacteria in patients with active pulmonary tuberculosis. Extensive neutrophilic response is associated with inflammation, pulmonary destruction, and pathology. For example, inappropriate phagocytosis of mycobacteria-infected neutrophils can damage host cells due to necrosis of neutrophils, leading to chronic inflammation and tissue damage. This dual nature of neutrophils makes them double-edged swords during tuberculosis, and hence data available on neutrophil functions against mycobacterium are controversial and non-uniform. This article reviews the role of neutrophils in tuberculosis infection and highlights research gaps that need to be addressed. We focus on our understanding of new research ideologies targeting neutrophils (a) in the early stages of infection for boosting specific immune functions or (b) in the later stages of infection to prevent inflammatory conditions mediated by activated neutrophils. This would plausibly lead to the development of better tuberculosis vaccines and therapeutics in the future.

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Section: Neutrophil Enzymesmentioning

confidence: 99%

Role of neutrophils in tuberculosis: A bird's eye view

et al. 2019

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“…This was most likely due to degranulation of cytotoxic agents. In vitro , M. tuberculosis induced neutrophils to release MPO and elastase ( 165 ) and neutrophils were found to release elastase and cathepsin G, another major azurophil granule protein, into the bronchoalveolar space in mice infected with M. bovis BCG ( 166 ). Released neutrophil granule proteins have been shown to be taken up by infected macrophages and to increase macrophage killing of M. tuberculosis and M. bovis BCG ( 35 , 164 ).…”

Section: Phagosomal Killing Of Mycobacteriamentioning

confidence: 99%

Antimicrobial Activity of Neutrophils Against Mycobacteria

et al. 2021

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The mycobacterium genus contains a broad range of species, including the human pathogens M. tuberculosis and M. leprae. These bacteria are best known for their residence inside host cells. Neutrophils are frequently observed at sites of mycobacterial infection, but their role in clearance is not well understood. In this review, we discuss how neutrophils attempt to control mycobacterial infections, either through the ingestion of bacteria into intracellular phagosomes, or the release of neutrophil extracellular traps (NETs). Despite their powerful antimicrobial activity, including the production of reactive oxidants such as hypochlorous acid, neutrophils appear ineffective in killing pathogenic mycobacteria. We explore mycobacterial resistance mechanisms, and how thwarting neutrophil action exacerbates disease pathology. A better understanding of how mycobacteria protect themselves from neutrophils will aid the development of novel strategies that facilitate bacterial clearance and limit host tissue damage.

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“…However, most applications of enzyme were limited by their instability; therefore, many efforts such as encapsulation and polymer modification have been performed to tackle this issue . The properties such as solubility, stability, and bioactivity of biological molecules can be specifically adjusted when modified with functional polymers , . Polymers with different hydrophilic/hydrophobic properties should have different influences on the properties of enzymes especially their stability and bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic bioactivity investigation of glucose oxidase modified with hydrophilic or hydrophobic polymers via in situ RAFT polymerization

et al. 2017

J. Polym. Sci. Part A: Polym. Chem.

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Biomacromolecules, such as enzymes are widely used for biocatalysis, both at academic and industrial level, due to their high specificity and wide applications in different reaction media. Herein, taking GOx as a representative enzyme, in‐situ RAFT polymerization of four different monomers including acrylic acid (AA), methyl acrylate (MA), poly (ethylene glycol) acrylate (PEG‐A) and tert‐butyl acrylate (TBA) were polymerized directly on the surface of GOx to afford GOx‐poly (PEG‐A)(GOx‐PPEG‐A), GOx‐poly(MA)(GOx‐PMA), GOx‐poly(AA)(GOx‐PAA), and GOx‐poly(TBA)(GOx‐PTBA) conjugates, respectively. Thereinto, PAA and PPEG‐A represent the hydrophilic polymers, while PMA and PTBA stand for the hydrophobic ones. Effects of different polymer on the properties of GOx were investigated by measuring the bioactivity and stability of the as‐prepared and different GOx‐polymer conjugates. Higher bioactivity was obtained for GOx modified with hydrophilic polymers compared with that modified with hydrophobic ones. All the tested polymers can enhance the stability of the GOx, while the hydrophobic GOx‐polymers conjugates exhibited much better stability than the hydrophilic ones. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 1289–1293

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Mycobacterium tuberculosis strains modify granular enzyme secretion and apoptosis of human neutrophils

Cited by 6 publications

References 31 publications

Role of neutrophils in tuberculosis: A bird's eye view

Role of neutrophils in tuberculosis: A bird's eye view

Antimicrobial Activity of Neutrophils Against Mycobacteria

Enzymatic bioactivity investigation of glucose oxidase modified with hydrophilic or hydrophobic polymers via in situ RAFT polymerization

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