Mycobacterium paratuberculosis Infection in a Colony of Stumptail Macaques (Macaca arctoides)

Hm, McClure; Rj, Chiodini; Dc, Anderson; Rb, Swenson; Wr, Thayer; Coutu, JA

doi:10.1093/infdis/155.5.1011

Cited by 128 publications

(82 citation statements)

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“…Two total genomic libraries of M. avium subsp. paratuberculosis DNA (strain 0138 ' Linda ' of human origin and 0142 of primate origin ; McClure et al, 1987) were cloned into pcDNAII expression vectors (Invitrogen) and screened for the presence of IS900 using PMB22\S12 or specific PCR primers as previously described (El-Zaatari et al, 1994 ;Pavlik et al, 1999). Fourteen distinct clones were identified by differences in PstI and XhoI restriction digest patterns.…”

Section: Methodsmentioning

confidence: 99%

“…M. avium subsp. paratuberculosis, which is very closely related to other M. avium subspecies, is a specific pathogen able to cause chronic inflammation of the intestine in ruminants, monogastrics such as dogs and pigs, and in subhuman primates (Larsen et al, 1971 ;McClure et al, 1987 ;Chiodini, 1989 ;Cocito et al, 1994). Our understanding of the genetic basis for this pathogenicity in M. avium subsp.…”

Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

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Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

et al. 2000

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Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was characterized and homologues in the Mycobacterium tuberculosis and M. avium subsp. avium genomes were identified. These included regions encoding a sigma factor (sigJ) at locus 3, a nitrate reductase (nirA) at locus 4, a transcription regulator (tetR) and polyketide synthase at locus 6, and a 6-O-methylguanine methyltransferase at locus 9. In addition, locus numbers were assigned to 9 of 15 RFLP bands previously described. IS900 insertion at 7 of the 14 characterized loci was into the RBS of a gene substituting an RBS encoded by IS900 sited two bases closer to the initiation codon. IS900 insertion at five loci interrupted an ORF at the target site, one of which encoded a homologue of the immunodominant mycobacterial DesA1 protein. Eleven of eighty-one M. avium subsp. paratuberculosis isolates lacked the insertion site at locus 6 together with flanking genomic DNA. This region was also absent from seven reference strains of M. avium subsp. avium, from one M. avium subsp. silvaticum and from six other mycobacterial species. A multiplex PCR of IS900 loci (MPIL) typing method was developed which was able to discriminate 10 different types of M. avium subsp. paratuberculosis from the panel of 81 isolates with consistent differences between those of bovine and ovine origin. Nine MPIL types corresponded with a single PstI/BstEII RFLP type, suggesting that this method may be applicable to typing of M. avium subsp. paratuberculosis directly from a sample without the need for culture. The remaining MPIL type corresponded with seven PstI/BstEII RFLP types. Further resolution of these may come from sequencing the remaining four uncharacterized IS900 loci.

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Section: Methodsmentioning

confidence: 99%

Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

et al. 2000

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“…UK. are close similarities of CD with Johne's disease, a chronic granulomatous enteritis of ruminants [8] and other animals [9] caused by Mycobacterium paratubercuhsis. The isolation and culture of Myco.…”

Section: Introductionmentioning

confidence: 99%

Peripheral cell-mediated immune response to mycobacterial antigens in inflammatory bowel disease

Rowbotham

Howdle

Trejdosiewicz

1995

Clinical and Experimental Immunology

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SUMMARYA mycobacterial etiology has been proposed In Crohn's disease (CD). We have sought evidence of increased or modified T lymphocyte immune responses to Mycnhactcrium tuberculosis and Myco. paratubercuhsis in patients with CD (ti = 13), compared with ulcerative colitis (UC; n = 17) and controls (« = 17). Peripheral blood cells were cultured with phytohaemagglutinin (positive mitogen control), mycobacterial purified protein derivative (PPD) preparations, lysates., column fractions and whole, heat-killed bacteria. Responses of T cells and T cell subsets were assessed by expression of activation markers (CD25. CD69). coupled with blast ogenesis assays (^H-thymidine uptake) and estimates of proliferation. Virtually all patients responded to Myco. paratuberculo.\is and Myco. tuberculosis antigens. There were no significant differences between patient groups, although there was a very high overall correlation (r = 095; P<0000\) between responses to the two mycobacterial species. Most of the activation and proliferative responses resided in the CD4'*' (T helper) subset. Although up to 15% of CDS"^ (suppressor/cytotoxic) cells also became activated, the CD8^ cells did not proliferate subsequently. Cells expressing the alternate y/) form of the T cell receptor (TCR 7(S^)did not activate or proliferate in response to mycobacterial antigens. There were no differences in any of these parameters between patient groups. We conclude that there is no specilic increase or alteration in cell-mediated anti-mycobacterial immunity in inflammatory bowel disease (IBD). Thus our data do not suppwrt a mycobacterial etiopathology of Crohn's disease.

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“…II) [166,167]. MAC agents have also been isolated from kangaroos, macaques and mandrills [79,105,115,163,180]. (iii) In many countries, the disease caused principally by Maa serotype 1,2,3, occurs in domestic and wild birds as well as in a variety of fowl, game birds and water-fowl (see Thorel et al.…”

Section: Wildlife As a Source Of The Mac Agentsmentioning

confidence: 99%

Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC)

Biet¹,

et al. 2005

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-Pathogens that are transmitted between the environment, wildlife, livestock and humans represent major challenges for the protection of human and domestic animal health, the economic sustainability of agriculture, and the conservation of wildlife. Among such pathogens, the genus Mycobacterium is well represented by M. bovis, the etiological agent of bovine tuberculosis, M. avium ssp. paratuberculosis (Map) the etiological agent of Johne disease, M. avium ssp. avium (Maa) and in a few common cases by other emergent environmental mycobacteria. Epidemiologic surveys performed in Europe, North America and New Zealand have demonstrated the existence and importance of environmental and wildlife reservoirs of mycobacterial infections that limit the attempts of disease control programmes. The aim of this review is to examine the zoonotic aspects of mycobacteria transmitted from the environment and wildlife. This work is focused on the species of two main groups of mycobacteria classified as important pathogens for humans and animals: first, M. bovis, the causative agent of bovine tuberculosis, which belongs to the M. tuberculosis complex and has a broad host range including wildlife, captive wildlife, domestic livestock, non-human primates and humans; the second group examined, is the M. avium-intracellulare complex (MAC) which includes M. avium ssp. avium causing major health problems in AIDS patients and M. avium ssp. paratuberculosis the etiological agent of Johne disease in cattle and identified in patients with Crohn disease. MAC agents, in addition to a broad host range, are environmental mycobacteria found in numerous biotopes including the soil, water, aerosols, protozoa, deep litter and fresh tropical vegetation. This review examines the possible reservoirs of these pathogens in the environment and in wildlife, their role as sources of infection in humans and animals and their health impact on humans. The possibilities of control and management programmes for these mycobacterial infections are examined with regards to the importance of their natural reservoirs.

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Mycobacterium paratuberculosis Infection in a Colony of Stumptail Macaques (Macaca arctoides)

Cited by 128 publications

References 9 publications

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

Peripheral cell-mediated immune response to mycobacterial antigens in inflammatory bowel disease

Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC)

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